Rabbit Anti-GRM1 Polyclonal Antibody – Cat# MBS502157

Metabotropic Glutamate Receptor 1a (GRM1) Antibody – Rabbit Polyclonal

Name: GRM1 Antibody; Polyclonal; Rabbit; Rat/Mouse-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS502157
Price: 495 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS502157

MBS502157 is an antibody targeting GRM1. Also known as Anti-Metabotropic Glutamate Receptor 1a. Reactive in Rat, mouse. Host: Rabbit. Affinity purified rabbit polyclonal antibody More details

$495 / 0.1 mL +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Metabotropic Glutamate Receptor 1a (GRM1), Polyclonal Antibody

Full Product Name

Anti-Metabotropic Glutamate Receptor 1a

Product Gene Name

anti-GRM1 antibody

Clonality

Polyclonal

Host

Rabbit

Species Reactivity

Rat, mouse

Form/Format

100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western blots

Purity/Purification

Affinity Purified (Prepared from rabbit serum by affinity purification using a column to which the peptide immunogen was coupled.)

Specificity

Specific for the ~125k monomer and the ~250k mGluR1a dimer. Immunolabeling blocked by preadsorption of antibody with the peptide used to generate the antibody.

Preparation and Storage

For long term storage -20 degree C is recommended. Stable at -20 degree C for at least 1 year.

Molecular Weight

125/250

Chromosome Location

Chromosome: 1; NC_005100.3 (6413420..6685561, complement). Location: 1p13

3D Structure

ModBase 3D Structure for P23385

Antigen

Peptide from the C-terminal region of rat mGluR1a.

Immunogen Information

Synthetic peptide corresponding to amino acid residues from the C-terminal region conjugated to KLH

Immunogen Species

Rat

Species Reactivity Note

The antibody has been directly tested for reactivity in Western blots in rat tissues.

Biological Significance

The metabotropic glutamate receptors (mGluRs) are key receptors in the modulation of excitatory synaptic transmission in the central nervous system. They are implicated in many forms of neural plasticity as well as learning and memory and drug abuse (Bhattacharya et al., 2004; Francesconi et al., 2004; Wilson and Nicoll, 2001). Group I metabotropic glutamate receptors (consisting of mGluR1 and mGluR5) are G-protein-coupled neurotransmitter receptors that are localized in the perisynaptic region of the postsynaptic membrane. When activated, Group I mGluRs lead to stimulation of phospholipase and activation of Protein Kinase C. In contrast, activation of Group II metabotropic receptors (mGluR2 and mGluR3) leads to inhibition of adenylate cyclase. The mGluR1 receptor may also be critically involved in limiting the deleterious effects of excitotoxicity (Blaabjerg et al., 2003).

Applications Tested/Suitable for anti-GRM1 antibody

Western Blot (WB), Immunohistochemistry (IHC)

Application Notes for anti-GRM1 antibody

Quality Control: Western blots performed on each lot.
WB: 1:1000
IHC (frozen sections; unpublished observations): 1:500

Product References and Citations for anti-GRM1 antibody

• Bhattacharya M, Babwah AV, Godin C, Anborgh PH, Dale LB, Poulter MO, Ferguson SSG (2004) Ral and phospholipase D2-dependent pathway for constitutive metabotropic glutamate receptor endocytosis. J Neurosci 24:8752-8761.
• Blaabjerg M, Fang LW, Zimmer J, Baskys A (2003) Neuroprotection against NMDA excitotoxicity by group I metabotropic glutamate receptors is associated with reduction of NMDA stimulated currents. Exp Neurol 183:573-580.
• Francesconi W, Cammalleri M, Sanna PP (2004) The metabotropic glutamate receptor 5 is necessary for late-phase long-term potentiation in the hippocampal CA1 region. Brain Res 1022:12-18.
• Wilson RI, Nicoll RA (2001) Endogenous cannabinoids mediate retrograde signalling at hippocampal synapses. Nature (London) 410:588-592.

Other Notes

Small volumes of anti-GRM1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for GRM1. It may not necessarily be applicable to this product.

NCBI GI #

166999225

NCBI GeneID

24414

NCBI Accession #

NP_001107802.1

UniProt Primary Accession #

P23385

UniProt Related Accession #

P23385

GenBank Nucleotide #

NM_001114330.1

NCBI Official Full Name

metabotropic glutamate receptor 1 isoform beta

NCBI Official Synonym Full Names

glutamate receptor, metabotropic 1

NCBI Official Symbol

Grm1

NCBI Official Synonym Symbols

Gprc1a

UniProt Protein Name

Metabotropic glutamate receptor 1

Protein Family

Metabotropic glutamate receptor

UniProt Gene Name

Grm1

UniProt Synonym Gene Names

Gprc1a; Mglur1; mGluR1

UniProt Entry Name

GRM1_RAT

NCBI Protein Information

metabotropic glutamate receptor 1; mGluR1; G protein coupled receptor family C group 1 member A

NCBI Summary for GRM1

may play a role in synaptic transmission; may modulate perception of pain in arthritis [RGD, Feb 2006]

UniProt Comments for GRM1

Function: G-protein coupled receptor for glutamate. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors. Signaling activates a phosphatidylinositol-calcium second messenger system. May participate in the central action of glutamate in the CNS, such as long-term potentiation in the hippocampus and long-term depression in the cerebellum. Ref.1 Ref.2 Ref.4

Subunit structure: Homodimer; disulfide-linked. The PPXXF motif binds HOMER1, HOMER2 and HOMER3. Interacts with RYR1, RYR2, ITPR1, SHANK1 and SHANK3

By similarity. Interacts with SIAH1 and GRASP. Ref.5 Ref.6 Ref.7 Ref.8

Subcellular location: Cell membrane; Multi-pass membrane protein.

Tissue specificity: Predominantly expressed in cerebellar Purkinje cells, CA2-CA3 pyramidal cells of the hippocampus, and mitral and tufted cells of the olfactory bulb. Ref.1 Ref.2

Miscellaneous: Activated by quisqualate > glutamate > ibotenate > trans-1- aminocyclopentyl-1,3-dicarboxylate; inhibited by 2-amino-3-phosphonopropionate.

Sequence similarities: Belongs to the G-protein coupled receptor 3 family.

Research Articles on GRM1

1. A calcium-dependent protein-protein interaction between a synaptic kinase and mGluR1a is found, constituting a feedback loop desensitizing mGluR1a.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-GRM1 antibody

Products by Pathway	Pathway Diagram
Calcium Signaling Pathway antibodies	Calcium Signaling Pathway Diagram
Calcium Signaling Pathway antibodies	Calcium Signaling Pathway Diagram
Class C/3 (Metabotropic Glutamate/pheromone Receptors) Pathway antibodies	Class C/3 (Metabotropic Glutamate/pheromone Receptors) Pathway Diagram
Estrogen Signaling Pathway antibodies	Estrogen Signaling Pathway Diagram
Estrogen Signaling Pathway antibodies	Estrogen Signaling Pathway Diagram
FoxO Signaling Pathway antibodies	FoxO Signaling Pathway Diagram
G Alpha (q) Signalling Events Pathway antibodies	G Alpha (q) Signalling Events Pathway Diagram
GPCR Downstream Signaling Pathway antibodies	GPCR Downstream Signaling Pathway Diagram
GPCR Ligand Binding Pathway antibodies	GPCR Ligand Binding Pathway Diagram
GPCRs, Class C Metabotropic Glutamate, Pheromone Pathway antibodies	GPCRs, Class C Metabotropic Glutamate, Pheromone Pathway Diagram

Diseases associated with anti-GRM1 antibody

Disease Name	Pubmed Publications
Nervous System Diseases Antibodies	>11 publications with GRM1 and Nervous System Diseases
Disease Models, Animal Antibodies	>9 publications with GRM1 and Disease Models, Animal
Brain Diseases Antibodies	>8 publications with GRM1 and Brain Diseases
Movement Disorders Antibodies	>3 publications with GRM1 and Movement Disorders
Tremor Antibodies	>2 publications with GRM1 and Tremor
Nerve Degeneration Antibodies	>1 publications with GRM1 and Nerve Degeneration
Cognition Disorders Antibodies	>1 publications with GRM1 and Cognition Disorders
Prenatal Exposure Delayed Effects Antibodies	>1 publications with GRM1 and Prenatal Exposure Delayed Effects
Epilepsy Antibodies	>1 publications with GRM1 and Epilepsy
Attention Deficit Disorder with Hyperactivity Antibodies	>1 publications with GRM1 and Attention Deficit Disorder with Hyperactivity

Organs/Tissues associated with anti-GRM1 antibody

Organ/Tissue Name	Pubmed Publications
Brain Antibodies	>16 publications with GRM1 and Brain