Rabbit Anti-GRIN2A Polyclonal Antibody – Cat# MBS502160

Western blot of rat hippocampal lysate showing specific immuno labeling of the ~180 kDa NR2A subunit of the NMDAR phosphorylated at Tyr1325 in the first lane (-). Phospho specificity is shown a in the second lane (+) where immuno labeling is completely eliminated by lysate treatment with lambda phosphatase (400 units/100uL lysate for 30 min).

NMDA NR2A Subunit (Tyr1325) (GRIN2A) Antibody – Rabbit Polyclonal

Name: GRIN2A Antibody; Polyclonal; Rabbit; Human/Mouse/Rat-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS502160
Price: 495 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS502160

MBS502160 is an antibody targeting GRIN2A. Also known as Anti-Phospho-Tyr1325 NMDA NR2A-Subunit. Reactive in Human, Mouse, Rat. Host: Rabbit. Affinity purified rabbit polyclonal antibody More details

$495 / 0.1 mL +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

NMDA NR2A Subunit (Tyr1325) (GRIN2A), Polyclonal Antibody

Full Product Name

Anti-Phospho-Tyr1325 NMDA NR2A-Subunit

Product Gene Name

anti-GRIN2A antibody

Clonality

Polyclonal

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Form/Format

100 ul in 150 mM NaCl, 10mM HEPES, 100ug per ml BSA and 50% glycerol, pH 7.5. Adequate amount of material to conduct 10 mini western blots.

Purity/Purification

Affinity Purified (Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.)

Specificity

Specific for the ~180k NMDAR NR2A-subunit protein phosphorylated at Tyr1325 in Western blots. Immunolabeling is completely blocked by blocked by the phosphopeptide used as the antigen but not by the corresponding dephosphopeptide.

Preparation and Storage

Store at -20 degree C in undiluted aliquots; stable for at least one year after date of receipt. Avoid freeze/thaw cycles.

Molecular Weight

~180 kDa

Chromosome Location

Chromosome: 10; NC_005109.3 (4523233..4940912). Location: 10q11

3D Structure

ModBase 3D Structure for Q00959

Antigen

Phosphopeptide corresponding to amino acid residues surrounding the phospho-Tyr1325 of rat NMDA NR2A.

Immunogen Information

Synthetic phospho-peptide corresponding to amino acid residues surrounding Tyr1325 conjugated to KLH

Quality Control

Western blots performed on each lot

Immunogen Species

Rat

Predicted Species Reactivity

Bovine, Canine, Hamster, Sheep. Predicted species reactivity is based on 100% homology with sequence used as antigen.

Species Reactivity Note

The antibody has been directly tested for reactivity in Western blots with human, mouse and rat tissues. It is anticipated that the antibody will react with bovine and canine based on the fact that these species have 100% homology with the amino acid sequence used as antigen.

Biological Significance

The ion channels activated by glutamate that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR). The NMDAR plays an essential role in memory, neuronal development and it has also been implicated in several disorders of the central nervous system including Alzheimer's, epilepsy and ischemic neuronal cell death (Grosshans et al., 2002; Wenthold et al., 2003; Carroll and Zukin, 2002). The NMDA receptor is also one of the principal molecular targets for alcohol in the CNS (Lovinger et al., 1989; Alvestad et al., 2003; Snell et al., 1996). Channels with physiological characteristics are produced when the NR1 subunit is combined with one or more of the NMDAR2 (NR2 A-D) subunits (Ishii et al., 1993). Recently, phosphorylation of Tyrosine 1325 of the NR2A subunit has been shown to be increased in human brain tissue sections from HIV-infected individuals with encephalitis (King et al., 2010). In addition, Tyr1325 phosphorylation has been linked with depression-related behavior (Taniguchi et al., 2009).

Applications Tested/Suitable for anti-GRIN2A antibody

Western Blot (WB)

Application Notes for anti-GRIN2A antibody

WB: 1:1000

Product References and Citations for anti-GRIN2A antibody

• Alvestad RM, Grosshans DR, Coultrap SJ, Nakazawa T, Yamamoto T, Browning MD (2003)Tyrosine dephosphorylation and ethanol inhibition of N-methyl-D-aspartate receptor function. J Biol Chem 278:11020-11025.
• Carroll RC, Zukin RS (2002) NMDA-receptor trafficking and targeting: implications for synaptic transmission and plasticity. Trends Neurosci 25:571-577.
• Grosshans DR, Clayton DA, Coultrap SJ, Browning MD (2002) LTP leads to rapid surface expression of NMDA but not AMPA receptors in adult rat CA1. Nat Neurosci 5:27-33. Ishii T, Moriyoshi K, Sugihara H, Sakurada K, Kadotani H, Yokoi M, Akazawa C, Shigemoto R, Mizuno N, Masu M, Nakanishi S (1993) Molecular characterization of the family of the N-methyl- D-aspartate receptor subunits. J Biol Chem 268:2836-2843. Lovinger DM, White G, Weight FF (1989) Ethanol inhibits NMDA-activated ion current in hippocampal neurons. Science 243:1721-1724. Snell LD, Nunley KR, Lickteig RL, Browning MD, Tabakoff B, Hoffman PL (1996) Regional and subunit specific changes in NMDA receptor mRNA and immunoreactivity in mouse brain following chronic ethanol ingestion. Mol Brain Res 40:71-78. Wenthold RJ, Prybylowski K, Standley S, Sans N, Petralia RS (2003) Trafficking of NMDA receptors. Annu Rev Pharmacol Toxicol 43:335-358. King JE, Eugenin EA, Hazleton JE, Morgello S, Berman JW (2010) Mechanisms of HIV-tat-induced phosphorylation of N-methyl-D-aspartate receptor subunit 2A in human primary neurons: implications for neuroAIDS pathogenesis. Am J Pathol. 176(6):2819-30.
• Taniguchi S, Nakazawa T, Tanimura A, Kiyama Y, Tezuka T, Watabe AM, Katayama N, Yokoyama K, Inoue T, Izumi-Nakaseko H, Kakuta S, Sudo K, Iwakura Y, Umemori H, Inoue T, Murphy NP, Hashimoto K, Kano M, Manabe T, Yamamoto T. (2009) Involvement of NMDAR2A tyrosine phosphorylation in depression-related behaviour. EMBO J. 28(23):3717-29.

Other Notes

Small volumes of anti-GRIN2A antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for GRIN2A. It may not necessarily be applicable to this product.

NCBI GI #

140971205

NCBI GeneID

24409

NCBI Accession #

NP_036705.3

UniProt Primary Accession #

Q00959

UniProt Secondary Accession #

O08948; Q63728

UniProt Related Accession #

Q00959

GenBank Nucleotide #

NM_012573.3

NCBI Official Full Name

glutamate receptor ionotropic, NMDA 2A

NCBI Official Synonym Full Names

glutamate receptor, ionotropic, N-methyl D-aspartate 2A

NCBI Official Symbol

Grin2a

NCBI Official Synonym Symbols

NR2A; GluN2A; NMDAR2A

UniProt Protein Name

Glutamate receptor ionotropic, NMDA 2A

UniProt Synonym Protein Names

Glutamate [NMDA] receptor subunit epsilon-1; N-methyl D-aspartate receptor subtype 2A

Protein Family

Glutamate receptor ionotropic

UniProt Gene Name

Grin2a

UniProt Synonym Gene Names

GluN2A; NMDAR2A; NR2A

UniProt Entry Name

NMDE1_RAT

NCBI Protein Information

glutamate receptor ionotropic, NMDA 2A; N-methyl D-aspartate receptor subtype 2A; N-methyl-D-aspartate receptor subunit 2A; glutamate [NMDA] receptor subunit epsilon-1

NCBI Summary for GRIN2A

may play a role in synaptic transmission, learning and memory [RGD, Feb 2006]

UniProt Comments for GRIN2A

Function: NMDA receptor subtype of glutamate-gated ion channels possesses high calcium permeability and voltage-dependent sensitivity to magnesium. Activation requires binding of agonist to both types of subunits.

Subunit structure: Forms heteromeric channel of a zeta subunit (GRIN1), a epsilon subunit (GRIN2A, GRIN2B, GRIN2C or GRIN2D) and a third subunit (GRIN3A or GRIN3B). Found in a complex with GRIN1, GRIN3A and PPP2CB. Interacts with AIP1. Found in a complex with GRIN1 and GRIN3B. Interacts with HIP1 and NETO1. Interacts with SNX27 (via PDZ domain); the interaction is required for recycling to the plasma membrane when endocytosed and prevent degradation in lysosomes

By similarity. Interacts with PDZ domains of INADL and DLG4. Interacts with LRFN2. Ref.5 Ref.6 Ref.7 Ref.8 Ref.9 Ref.10 Ref.11 Ref.12

Subcellular location: Cell membrane; Multi-pass membrane protein. Cell junction › synapse › postsynaptic cell membrane; Multi-pass membrane protein Ref.8.

Sequence similarities: Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. NR2A/GRIN2A subfamily. [View classification]

Research Articles on GRIN2A

1. we examined the contribution of NR2B- and NR2A-containing NMDARs to the formation of trace and contextual memory in the prelimbic medial prefrontal cortex

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-GRIN2A antibody

Products by Pathway	Pathway Diagram
Activation Of NMDA Receptor Upon Glutamate Binding And Postsynaptic Events Pathway antibodies	Activation Of NMDA Receptor Upon Glutamate Binding And Postsynaptic Events Pathway Diagram
Alcoholism Pathway antibodies	Alcoholism Pathway Diagram
Alcoholism Pathway antibodies	Alcoholism Pathway Diagram
Alzheimer's Disease Pathway antibodies	Alzheimer's Disease Pathway Diagram
Alzheimer's Disease Pathway antibodies	Alzheimer's Disease Pathway Diagram
Amphetamine Addiction Pathway antibodies	Amphetamine Addiction Pathway Diagram
Amphetamine Addiction Pathway antibodies	Amphetamine Addiction Pathway Diagram
Amyotrophic Lateral Sclerosis (ALS) Pathway antibodies	Amyotrophic Lateral Sclerosis (ALS) Pathway Diagram
Amyotrophic Lateral Sclerosis (ALS) Pathway antibodies	Amyotrophic Lateral Sclerosis (ALS) Pathway Diagram
CREB Phosphorylation Through The Activation Of CaMKII Pathway antibodies	CREB Phosphorylation Through The Activation Of CaMKII Pathway Diagram

Diseases associated with anti-GRIN2A antibody

Disease Name	Pubmed Publications
Nervous System Diseases Antibodies	>15 publications with GRIN2A and Nervous System Diseases
Brain Diseases Antibodies	>14 publications with GRIN2A and Brain Diseases
Epilepsy Antibodies	>12 publications with GRIN2A and Epilepsy
Melanoma Antibodies	>6 publications with GRIN2A and Melanoma
Movement Disorders Antibodies	>6 publications with GRIN2A and Movement Disorders
Landau-Kleffner Syndrome Antibodies	>5 publications with GRIN2A and Landau-Kleffner Syndrome
Epilepsy, Rolandic Antibodies	>5 publications with GRIN2A and Epilepsy, Rolandic
Mood Disorders Antibodies	>5 publications with GRIN2A and Mood Disorders
Speech Disorders Antibodies	>3 publications with GRIN2A and Speech Disorders
Cognition Disorders Antibodies	>3 publications with GRIN2A and Cognition Disorders