Metabotropic Glutamate Receptor 7 (Ser862) (GRM7) Antibody – Rabbit Polyclonal

Polyclonal

Rabbit

Rat, mouse

100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug BSA per ml and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

Affinity Purified (Prepared from rabbit serum by affinity purification via sequential chromatography on phospho and dephospho-peptide columns.)

Specific for the ~102k mGluR7 protein phosphorylated at Ser862. Immunolabeling is blocked by preadsorption of antibody with the phospho-peptide used as antigen but not by the corresponding dephosphopeptide.

For long term storage -20 degree C is recommended. Stable at -20 degree C for at least 1 year.

102

Chromosome: 4; NC_005103.3 (205768625..206090308). Location: 4q42

ModBase 3D Structure for P35400

Phosphopeptide corresponding to amino acid residues surrounding phospho-Ser862 of rat mGluR7.

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser862 conjugated to KLH

Rat

Bovine, canine, human, non-human primate and zebrafish

The antibody has been directly tested for reactivity in Western blots in mouse and rat tissues. It is anticipated that the antibody will also work with bovine, canine, human, non-human primate and zebrafish tissues based on the fact that these species have 100% homology with the amino acid sequence used as antigen.

Metabotropic glutamate receptors (mGluRs) are key receptors in the modulation of excitatory synaptic transmission in the central nervous system. They are implicated in many forms of neural plasticity as well as learning and memory and drug abuse (Bhattacharya et al., 2004; Francesconi et al., 2004; Wilson and Nicoll, 2001). The mGluRs are divided into three groups based on sequence identity and pharmacological properties: group I (mGluR1 and mGluR5) are localized in the perisynaptic region of the postsynaptic membrane, whereas group II (mGlur2 and mGluR3) and group III (mGluR4,6,7 and 8) are localized predominantly at presynaptic terminals. PKC phosphorylation of serine 862 on mGluR7 has been shown to be critical for stabilizing receptor surface expression and promoting binding to the synaptic PDZ-domain-containing protein PICK1 (Suh et al., 2008).