Rabbit Anti-TNNI3 Polyclonal Antibody – Cat# MBS502174

Troponin I (Cardiac) Ser23/24 (TNNI3) Antibody – Rabbit Polyclonal

Name: TNNI3 Antibody; Polyclonal; Rabbit; Mouse/Rat-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS502174
Price: 495 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS502174

MBS502174 is an antibody targeting TNNI3. Also known as Anti-Phospho-Ser23/24 Troponin I (cardiac). Reactive in Mouse, rat. Host: Rabbit. Affinity purified rabbit polyclonal antibody More details

$495 / 0.1 mL +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Troponin I (cardiac) Ser23/24 (TNNI3), Polyclonal Antibody

Full Product Name

Anti-Phospho-Ser23/24 Troponin I (cardiac)

Product Gene Name

anti-TNNI3 antibody

Clonality

Polyclonal

Host

Rabbit

Species Reactivity

Mouse, rat

Form/Format

100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots

Purity/Purification

Affinity Purified (Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephospho-peptide affinity columns.)

Specificity

Specific for the ~25k cardiac troponin I protein phosphorylated at Ser23/24.

Preparation and Storage

Store at -20 degree C; stable for at least 1 year

Molecular Weight

Chromosome Location

Chromosome: 7; NC_000073.6 (4518308..4522443, complement). Location: 7 A1; 7 2.61 cM

3D Structure

ModBase 3D Structure for P48787

Antigen

Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser23/24 of mouse troponin I, cardiac (cTnI).

Immunogen Information

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser23/24 conjugated to KLH

Immunogen Species

Mouse

Reactivity Assumed Based on 100% Sequence Homology

Human, non-human primate

Species Reactivity Note

The antibody has been directly tested for reactivity in Western blots with mouse and rat tissue. It is anticipated that the antibody will react with human and non-human primates based on the fact that these species have 100% homology with the amino acid sequence used as antigen.

Biological Significance

Applications Tested/Suitable for anti-TNNI3 antibody

Western Blot (WB)

Application Notes for anti-TNNI3 antibody

Quality Control: Western blots performed on each lot.
WB: 1:1000

Product References and Citations for anti-TNNI3 antibody

• Thomas A. Noland, Jr., Xiaodu Guo, Robert L. Raynor, Nathan M. Jideama, Vera Averyhart-Fullard, R. John Solaro, and J.F. Kuo (1995) Cardiac Troponin I Mutants. J of Biol Chem 270 (43): 25445-25454.
• Viola Kooij, Martina Saes, Kornelia Jaquet, Ruud Zaremba, D. Brian Foster, Anne M. Murphy, Cris dos Remedios, Joland van der Velden, Ger J.M. Stienen (2010) Effect of Troponin I ser23/24 phosphorylation on Ca2+-sensitivity in human myocardium depends on the phosphorylation background. J of Mol Cell Card 48: 954-963.
• Margaret V. Westfall, Adonia M. Lee, and Dustin A. Robinson (2005) Differential Contribution of Troponin I Phosphorylation Sites to the Endothelin-modulated Contractile Response. J of Biol Chem 280(50): 41324-41331.

Other Notes

Small volumes of anti-TNNI3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for TNNI3. It may not necessarily be applicable to this product.

NCBI GI #

6678393

NCBI GeneID

21954

NCBI Accession #

NP_033432.1

UniProt Primary Accession #

P48787

UniProt Related Accession #

P48787

GenBank Nucleotide #

NM_009406.3

NCBI Official Full Name

troponin I, cardiac muscle

NCBI Official Synonym Full Names

troponin I, cardiac 3

NCBI Official Symbol

Tnni3

NCBI Official Synonym Symbols

Tn1; cTnI

UniProt Protein Name

Troponin I, cardiac muscle

UniProt Synonym Protein Names

Cardiac troponin I

Protein Family

Troponin

UniProt Gene Name

Tnni3

UniProt Entry Name

TNNI3_MOUSE

NCBI Protein Information

troponin I, cardiac muscle; cardiac troponin I

UniProt Comments for TNNI3

Function: Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.

Subunit structure: Interacts with TRIM63

By similarity. Binds to actin and tropomyosin. Interacts with STK4/MST1

By similarity.

Post-translational modification: Phosphorylated at Ser-23 and Ser-24 by PRKD1; phosphorylation reduces myofilament calcium sensitivity. Phosphorylated preferentially at Thr-32. Phosphorylation by STK4/MST1 alters its binding affinity to TNNC1 (cardiac Tn-C) and TNNT2 (cardiac Tn-T)

By similarity. Phosphorylated at Ser-43 and Ser-45 by PRKCE; phosphorylation increases myocardium contractile dysfunction. Ref.4 Ref.5

Sequence similarities: Belongs to the troponin I family.

Research Articles on TNNI3

1. Conclude that dilated cardiomyopathy-causing mutations in thin filament proteins abolish the relationship between myofilament Ca(2+) sensitivity and troponin I phosphorylation by PKA.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-TNNI3 antibody

Reference Product	PubMed Publications
Tnnt2 antibody	>51 publications with TNNI3 and Tnnt2
Mybpc3 antibody	>44 publications with TNNI3 and Mybpc3
Tpm1 antibody	>28 publications with TNNI3 and Tpm1
Myl2 antibody	>20 publications with TNNI3 and Myl2
Myl3 antibody	>20 publications with TNNI3 and Myl3
Tnnc1 antibody	>11 publications with TNNI3 and Tnnc1
Actc1 antibody	>8 publications with TNNI3 and Actc1
Tcap antibody	>3 publications with TNNI3 and Tcap
Tnnt3 antibody	>2 publications with TNNI3 and Tnnt3

Pathways associated with anti-TNNI3 antibody

Products by Pathway	Pathway Diagram
Adrenergic Signaling In Cardiomyocytes Pathway antibodies	Adrenergic Signaling In Cardiomyocytes Pathway Diagram
Adrenergic Signaling In Cardiomyocytes Pathway antibodies	Adrenergic Signaling In Cardiomyocytes Pathway Diagram
Cardiac Muscle Contraction Pathway antibodies	Cardiac Muscle Contraction Pathway Diagram
Cardiac Muscle Contraction Pathway antibodies	Cardiac Muscle Contraction Pathway Diagram
Dilated Cardiomyopathy Pathway antibodies	Dilated Cardiomyopathy Pathway Diagram
Dilated Cardiomyopathy Pathway antibodies	Dilated Cardiomyopathy Pathway Diagram
Hypertrophic Cardiomyopathy (HCM) Pathway antibodies	Hypertrophic Cardiomyopathy (HCM) Pathway Diagram
Hypertrophic Cardiomyopathy (HCM) Pathway antibodies	Hypertrophic Cardiomyopathy (HCM) Pathway Diagram
Muscle Contraction Pathway antibodies	Muscle Contraction Pathway Diagram
Striated Muscle Contraction Pathway antibodies	Striated Muscle Contraction Pathway Diagram

Diseases associated with anti-TNNI3 antibody

Disease Name	Pubmed Publications
Cardiovascular Diseases Antibodies	>70 publications with TNNI3 and Cardiovascular Diseases
Heart Diseases Antibodies	>69 publications with TNNI3 and Heart Diseases
Cardiomyopathies Antibodies	>61 publications with TNNI3 and Cardiomyopathies
Cardiomyopathy, Hypertrophic, Familial Antibodies	>25 publications with TNNI3 and Cardiomyopathy, Hypertrophic, Familial
Cardiomyopathy, Dilated Antibodies	>18 publications with TNNI3 and Cardiomyopathy, Dilated
CARDIOMYOPATHY, FAMILIAL HYPERTROPHIC, 7 Antibodies	>9 publications with TNNI3 and CARDIOMYOPATHY, FAMILIAL HYPERTROPHIC, 7
Cardiomyopathy, Restrictive Antibodies	>8 publications with TNNI3 and Cardiomyopathy, Restrictive
Nervous System Diseases Antibodies	>5 publications with TNNI3 and Nervous System Diseases
Cardiomyopathy, Familial Restrictive, 1 Antibodies	>3 publications with TNNI3 and Cardiomyopathy, Familial Restrictive, 1
Hypertension Antibodies	>3 publications with TNNI3 and Hypertension

Organs/Tissues associated with anti-TNNI3 antibody

Organ/Tissue Name	Pubmed Publications
Heart Antibodies	>55 publications with TNNI3 and Heart
Muscle Antibodies	>54 publications with TNNI3 and Muscle
Lung Antibodies	>2 publications with TNNI3 and Lung
Embryonic Tissue Antibodies	>2 publications with TNNI3 and Embryonic Tissue
Brain Antibodies	>1 publications with TNNI3 and Brain
Kidney Antibodies	>1 publications with TNNI3 and Kidney