Rabbit Anti-SNAP25 Polyclonal Antibody – Cat# MBS502175

SNAP25 Antibody – Rabbit Polyclonal

Name: SNAP25 Antibody; Polyclonal; Rabbit; Rat-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS502175
Price: 495 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS502175

MBS502175 is an antibody targeting SNAP25. Also known as Anti-Phospho-Ser187 SNAP25. Reactive in Rat. Host: Rabbit. Affinity purified rabbit polyclonal antibody More details

$495 / 0.1 mL +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

SNAP25 (Ser187), Polyclonal Antibody

Full Product Name

Anti-Phospho-Ser187 SNAP25

Product Gene Name

anti-SNAP25 antibody

Clonality

Polyclonal

Host

Rabbit

Species Reactivity

Rat

Form/Format

100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug BSA per ml and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

Purity/Purification

Affinity Purified (Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.)

Specificity

Specific for the ~25k SNAP25 protein phosphorylated at Ser187 in Western blots. Immunolabeling is completely blocked by blocked lambda-Ptase.

Preparation and Storage

For long term storage -20 degree C is recommended. Stable at -20 degree C for at least 1 year.

Molecular Weight

Chromosome Location

Chromosome: 3; NC_005102.3 (136182051..136269422). Location: 3q36

3D Structure

ModBase 3D Structure for P60881

Antigen

Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser187 of SNAP25

Immunogen Information

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser187 conjugated to KLH

Immunogen Species

Rat

Reactivity Assumed Based on 100% Sequence Homology

Guinea pig, human, mouse, non-human primate, zebra fish

Species Reactivity Note

The antibody has been directly tested for reactivity in Western blots of rat tissue. It is anticipated that the antibody will work with guinea pig, human, mouse, non-human primate and zebra fish tissue due to the fact that these species have 100 % homology with the amino acid sequence used as antigen

Biological Significance

SNAP25 (Synaptosomal associated protein of 25 kDa) is a presynaptic plasma membrane protein that is widely distributed throughout the brain and involved in the regulation of neurotransmitter release. Decreased levels of SNAP25 have been found in the brains of patients with Down Syndrome and Alzheimer's Disease (Greber et al.,1999). In addition, a significant reduction in the hippocampal expression of SNAP25 has also been found in patients with Schizophrenia (Fatemi et al., 2001). Increasing evidence suggests that SNAP-25 also modulates various ion channels, including voltage gated calcium channels (VGCCs) (Pozzi el al., 2008). Activation of PKC results in the phosphorylation of SNAP-25 on ser187 (Shu et al., 2008). Phosphorylation of SNAP25 on ser187 is believed to cause inhibition of VGCC (Pozzi el al., 2008). Since ser187 phosphorylation is transiently induced by neuronal activity, SNAP25 creates a negative feedback mechanism for controlling neuronal excitability (Pozzi el al., 2008).

Applications Tested/Suitable for anti-SNAP25 antibody

Western Blot (WB)

Application Notes for anti-SNAP25 antibody

Quality Control: Western blots performed on each lot.
WB: 1:1000

Product References and Citations for anti-SNAP25 antibody

• Ando K, Kudo Y, Takahashi M (2005) Negative regulation of neurotransmitter release by calpain: a possible specific involvement of Snap25 cleavage. J Neurochem 94(3):651-8.
• Fatemi SH, Earle JA, Stary JM, Lee S, Sedgwick J (2001) Altered levels of the synaptosomal associated protein Snap25 in hippocampus of patients with mood disorders and Schizophrenia. Neuroreport. 12(15): 3257-62.
• Fatemi SH, Sidwell R, Kist D, Akhter P, Meltzer HY, Bailey K, Thuras P, Sedgwick J (1998) Differential expression of synaptosome-associated protein 25 kDa (Snap25) in hippocampi of neonatal mice following exposure to human influenza virus in utero. Brain Res 800(1):1-9.
• Greber S, Cairns N, Lubec G, Fountoulakis M (1999) Decreased levels of the synaptosomal associated protein 25 in the brains of patients with Down Syndrome and Alzheimer's Disease. Electrophoresis 20 (4-5):928-34.
• Pozzi D, Condliffe S, Bozzi Y, Chikhladze M, Grumelli C, Proux-Gillardeaux V, Takahashi M, Franceschetti S, Verderio C, Matteoli M (2008) Activity-dependent phosphorylation of Ser187 is required for SNAP-25-negative modulation of neuronal voltage-gated calcium channels. PNAS 105: 323-328.

Other Notes

Small volumes of anti-SNAP25 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for SNAP25. It may not necessarily be applicable to this product.

NCBI GI #

396080314

NCBI GeneID

25012

NCBI Accession #

NP_001257504.1

UniProt Primary Accession #

P60881

UniProt Secondary Accession #

P13795; P36974; P70557; P70558; Q8IXK3; Q96FM2; Q9BR45

UniProt Related Accession #

P60881

GenBank Nucleotide #

NM_001270575.1

NCBI Official Full Name

synaptosomal-associated protein 25 isoform a

NCBI Official Synonym Full Names

synaptosomal-associated protein 25

NCBI Official Symbol

Snap25

NCBI Official Synonym Symbols

SNAP-25B; SNAP-25a

UniProt Protein Name

Synaptosomal-associated protein 25

UniProt Synonym Protein Names

Super protein; SUP; Synaptosomal-associated 25 kDa protein

Protein Family

Synaptosomal-associated protein

UniProt Gene Name

Snap25

UniProt Synonym Gene Names

Snap; SNAP-25; SUP

UniProt Entry Name

SNP25_RAT

NCBI Protein Information

synaptosomal-associated protein 25; SUP; SNAP-25; super protein; synaptosomal-associated 25 kDa protein; synaptosomal-associated protein, 25 kDa

NCBI Summary for SNAP25

Synaptic vesicle membrane docking and fusion is mediated by SNAREs (soluble N-ethylmaleimide-sensitive factor attachment protein receptors) located on the vesicle membrane (v-SNAREs) and the target membrane (t-SNAREs). The assembled v-SNARE/t-SNARE complex consists of a bundle of four helices, one of which is supplied by v-SNARE and the other three by t-SNARE. For t-SNAREs on the plasma membrane, the protein syntaxin supplies one helix and the protein encoded by this gene contributes the other two. Therefore, this gene product is a presynaptic plasma membrane protein. It is essential for regulated exocytosis in neuronal cells. Alternative transcript variants encoding two different protein isoforms have been described for this gene. [provided by RefSeq, Jul 2012]

UniProt Comments for SNAP25

Function: t-SNARE involved in the molecular regulation of neurotransmitter release. May play an important role in the synaptic function of specific neuronal systems. Associates with proteins involved in vesicle docking and membrane fusion. Regulates plasma membrane recycling through its interaction with CENPF.

Subunit structure: Interacts with CENP and OTOF. Found in a complex containing SYT1, SV2B and syntaxin-1. Associates with the BLOC-1 complex. Isoform 1 and isoform 2 interact with BLOC1S6

By similarity. Part of the SNARE core complex containing SNAP25, VAMP2 and STX1A. This complex binds CPLX1. Interacts with TRIM9, RIMS1, SNAPIN, and HGS. Binds STXBP6. Found in a ternary complex with STX1A and VAMP8. Interacts with EQTN

By similarity. Ref.9 Ref.10 Ref.11 Ref.12

Subcellular location: Cytoplasm › perinuclear region

By similarity. Cell membrane; Lipid-anchor. Cell junction › synapse › synaptosome. Note: Membrane association requires palmitoylation. Expressed throughout cytoplasm, concentrating at the perinuclear region

By similarity. Ref.6 Ref.8

Post-translational modification: Palmitoylated. Cys-85 appears to be the main site, and palmitoylation is required for membrane association

By similarity. Ref.6

Sequence similarities: Belongs to the SNAP-25 family.Contains 2 t-SNARE coiled-coil homology domains.

Research Articles on SNAP25

1. protein is mostly important in tissues outside the brain. Our developmental data showed a low expression of Snap25 during fetal life with a sharp increase in the expression of Snap25 just after birth, between postnatal day 2 and 6.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-SNAP25 antibody

Products by Pathway	Pathway Diagram
Acetylcholine Neurotransmitter Release Cycle Pathway antibodies	Acetylcholine Neurotransmitter Release Cycle Pathway Diagram
GABA Synthesis, Release, Reuptake And Degradation Pathway antibodies	GABA Synthesis, Release, Reuptake And Degradation Pathway Diagram
Glutamate Neurotransmitter Release Cycle Pathway antibodies	Glutamate Neurotransmitter Release Cycle Pathway Diagram
Insulin Signaling Pathway antibodies	Insulin Signaling Pathway Diagram
Insulin Secretion Pathway antibodies	Insulin Secretion Pathway Diagram
Neuronal System Pathway antibodies	Neuronal System Pathway Diagram
Neurotransmitter Release Cycle Pathway antibodies	Neurotransmitter Release Cycle Pathway Diagram
Norepinephrine Neurotransmitter Release Cycle Pathway antibodies	Norepinephrine Neurotransmitter Release Cycle Pathway Diagram
SNARE Interactions In Vesicular Transport Pathway antibodies	SNARE Interactions In Vesicular Transport Pathway Diagram
SNARE Interactions In Vesicular Transport Pathway antibodies	SNARE Interactions In Vesicular Transport Pathway Diagram

Diseases associated with anti-SNAP25 antibody

Disease Name	Pubmed Publications
Nervous System Diseases Antibodies	>119 publications with SNAP25 and Nervous System Diseases
Paralysis Antibodies	>21 publications with SNAP25 and Paralysis
Pain Antibodies	>17 publications with SNAP25 and Pain
Cognition Disorders Antibodies	>17 publications with SNAP25 and Cognition Disorders
Memory Disorders Antibodies	>15 publications with SNAP25 and Memory Disorders
Movement Disorders Antibodies	>13 publications with SNAP25 and Movement Disorders
Seizures Antibodies	>12 publications with SNAP25 and Seizures
Neurobehavioral Manifestations Antibodies	>12 publications with SNAP25 and Neurobehavioral Manifestations
Learning Disorders Antibodies	>12 publications with SNAP25 and Learning Disorders
Diabetes Mellitus Antibodies	>10 publications with SNAP25 and Diabetes Mellitus

Organs/Tissues associated with anti-SNAP25 antibody

Organ/Tissue Name	Pubmed Publications
Brain Antibodies	>430 publications with SNAP25 and Brain
Pancreas Antibodies	>52 publications with SNAP25 and Pancreas
Eye Antibodies	>34 publications with SNAP25 and Eye
Pituitary Gland Antibodies	>22 publications with SNAP25 and Pituitary Gland
Adrenal Gland Antibodies	>15 publications with SNAP25 and Adrenal Gland
Inner Ear Antibodies	>12 publications with SNAP25 and Inner Ear
Dorsal Root Ganglion Antibodies	>10 publications with SNAP25 and Dorsal Root Ganglion
Pineal Gland Antibodies	>4 publications with SNAP25 and Pineal Gland
Prostate Antibodies	>1 publications with SNAP25 and Prostate