Chicken Anti-GAP43 Polyclonal Antibody – Cat# MBS502179

GAP43 Antibody – Chicken Polyclonal

Name: GAP43 Antibody; Polyclonal; Chicken; MyBioSource
Brand: MyBioSource
SKU: MBS502179
Price: 435 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS502179

MBS502179 is an antibody targeting GAP43. Also known as Anti-GAP-43. Reactive in Bovine , Human, Mouse, Pig, Rat. Host: Chicken. Chicken polyclonal antibody More details

$435 / 0.1 mL +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

GAP43, Polyclonal Antibody

Full Product Name

Anti-GAP-43

Product Gene Name

anti-GAP43 antibody

Clonality

Polyclonal

Host

Chicken

Species Reactivity

Bovine , Human, Mouse, Pig, Rat

Form/Format

Total IgY fraction in PBS + 10 mM NaN3.

Purity/Purification

Total IgY fraction

Specificity

Specific for the ~43k GAP-43 protein.

Preparation and Storage

For long term storage -20 degree C is recommended. Stable at -20 degree C for at least 1 year

Molecular Weight

~43

Chromosome Location

Chromosome: 11; NC_005110.3 (62777623..62870998). Location: 11q21

3D Structure

ModBase 3D Structure for P07936

Packaging

Adequate amount of material to conduct 10-mino Western Blots.

Antigen

Synthetic peptide corresponding to amino acid residues from the C-terminal region of the rat GAP43 sequence.

Antigen Specificity

Specific for endogenous levels of the !43 kDa GAP-43 protein

Immunogen Information

Synthetic peptide corresponding to amino acid residues from the C-terminal region conjugated to KLH

Immunogen Species

Rat

Species Reactivity Note

The antibody has been directly tested for reactivity in Western blots with human, mouse and rat tissue.

Biological Significance

GAP-43 is thought to have an important role in development and plasticity because it is expressed at high levels in neuronal growth cones during development and during axonal regeneration (Benowitz and Routtenberg, 1997). There is also evidence from knockout animals that GAP-43 serves to amplify pathfinding signals from the growth cone (Strittmatter et al., 1995). GAP-43 is thought to mediate at least some of these effects via interaction with actin. Importantly, phosphorylation at Ser41 by protein kinase C modulates the interaction of GAP-43 with actin (He et al., 1997) and may also affect neurotransmitter release during forms of plasticity like LTP (Hulo et al., 2002).

Applications Tested/Suitable for anti-GAP43 antibody

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Application Notes for anti-GAP43 antibody

Quality Control: Western blots performed on each lot.
WB: 1:2000
IF: 1:500

Product References and Citations for anti-GAP43 antibody

• Benowitz LI, Routtenberg A (1997) Gap-43: An intrinsic determinant of neuronal development and plasticit. Trends Neurosci 20:84-91.
• He, Q, Dent, EW, Meiri, KF (1997) Modulation of actin filament behavior by Gap-43 (neuromodulin) is dependent on the phosphorylation status of serine 41, the protein kinase C site. J Neurosci 17:3515-3524.
• Hulo S, Alberi, S, Laux T, Muller D, Caroni P (2002) A point mutant of Gap-43 induces enhanced short-term and long-term hippocampal plasticity. Eur J Neurosci 15:1976-1982.
• Strittmatter SM, Fankhauser C, Huang PL, Mashimo H, Fishman MC (1995) Neuronal path finding is abnormal in mice lacking the neuronal growth cone protein Gap-43," Cell 80:445-452.
• Rayudu Gopalakrishna, Usha Gundimeda, Jason Eric Schiffman, and Thomas H. McNeill (2008) A Direct Redox Regulation of Protein Kinase C Isoenzymes Mediates Oxidant-induced Neuritogenesis in PC12 Cells J. Biol. Chem., May 2008; 283: 14430 - 14444.

Other Notes

Small volumes of anti-GAP43 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for GAP43. It may not necessarily be applicable to this product.

NCBI GI #

8393415

NCBI GeneID

29423

NCBI Accession #

NP_058891.1

UniProt Primary Accession #

P07936

UniProt Secondary Accession #

A0JPM4

UniProt Related Accession #

P07936

GenBank Nucleotide #

NM_017195.3

NCBI Official Full Name

neuromodulin

NCBI Official Synonym Full Names

growth associated protein 43

NCBI Official Symbol

Gap43

NCBI Official Synonym Symbols

Basp2

UniProt Protein Name

Neuromodulin

UniProt Synonym Protein Names

Axonal membrane protein GAP-43; Growth-associated protein 43; Protein F1

Protein Family

Neuromodulin

UniProt Gene Name

Gap43

UniProt Entry Name

NEUM_RAT

NCBI Protein Information

neuromodulin; protein F1; growth-associated protein 43; axonal membrane protein GAP-43; growth accentuating protein 43; brain abundant, membrane attached signal protein 2

NCBI Summary for GAP43

may play a role in neurite outgrowth and nerve regeneration [RGD, Feb 2006]

UniProt Comments for GAP43

Function: This protein is associated with nerve growth. It is a major component of the motile "growth cones" that form the tips of elongating axons. Plays a role in axonal and dendritic filopodia induction

By similarity.

Subunit structure: Identified in a complex containing FGFR4, NCAM1, CDH2, PLCG1, FRS2, SRC, SHC1, GAP43 and CTTN

By similarity. Binds calmodulin with a greater affinity in the absence of Ca2+ than in its presence

By similarity.

Subcellular location: Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cell projection › growth cone membrane; Peripheral membrane protein; Cytoplasmic side. Cell junction › synapse. Cell projection › filopodium membrane; Peripheral membrane protein

By similarity. Note: Cytoplasmic surface of growth cone and synaptic plasma membranes.

Post-translational modification: Palmitoylation by ARF6 is essential for plasma membrane association and axonal and dendritic filopodia induction. Deacylated by LYPLA2

By similarity.Phosphorylated at Ser-41 by PHK. Phosphorylation of this protein by a protein kinase C is specifically correlated with certain forms of synaptic plasticity. Ref.7 Ref.8 Ref.9

Sequence similarities: Belongs to the neuromodulin family.Contains 1 IQ domain.

Research Articles on GAP43

1. an AU-rich regulatory element (ARE) in GAP-43's 3'UTR is necessary and sufficient for axonal localization of Zbp1

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-GAP43 antibody

Products by Pathway	Pathway Diagram
Spinal Cord Injury Pathway antibodies	Spinal Cord Injury Pathway Diagram

Diseases associated with anti-GAP43 antibody

Disease Name	Pubmed Publications
Nervous System Diseases Antibodies	>112 publications with GAP43 and Nervous System Diseases
Disease Models, Animal Antibodies	>56 publications with GAP43 and Disease Models, Animal
Brain Diseases Antibodies	>47 publications with GAP43 and Brain Diseases
Cardiovascular Diseases Antibodies	>39 publications with GAP43 and Cardiovascular Diseases
Neoplasms Antibodies	>32 publications with GAP43 and Neoplasms
Death Antibodies	>30 publications with GAP43 and Death
Heart Diseases Antibodies	>28 publications with GAP43 and Heart Diseases
Inflammation Antibodies	>21 publications with GAP43 and Inflammation
Nerve Degeneration Antibodies	>21 publications with GAP43 and Nerve Degeneration
Brain Injuries Antibodies	>20 publications with GAP43 and Brain Injuries

Organs/Tissues associated with anti-GAP43 antibody

Organ/Tissue Name	Pubmed Publications
Nerve Antibodies	>247 publications with GAP43 and Nerve
Brain Antibodies	>208 publications with GAP43 and Brain
Heart Antibodies	>33 publications with GAP43 and Heart
Eye Antibodies	>28 publications with GAP43 and Eye
Dorsal Root Ganglion Antibodies	>27 publications with GAP43 and Dorsal Root Ganglion
Colon Antibodies	>1 publications with GAP43 and Colon
Pituitary Gland Antibodies	>1 publications with GAP43 and Pituitary Gland
Connective Tissue Antibodies	>1 publications with GAP43 and Connective Tissue