Rabbit Anti-XIRP1 Polyclonal Antibody – Cat# MBS502180

Xin Actin-Binding Repeat-Containing Protein 1 (XIRP1) (Ser295) (XIRP1) Antibody – Rabbit Polyclonal

Name: XIRP1 Antibody; Polyclonal; Rabbit; Mouse-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS502180
Price: 495 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS502180

MBS502180 is an antibody targeting XIRP1. Also known as Anti-Phospho-Ser295 Xin Actin-Binding Repeat-Containing Protein1 (XIRP1). Reactive in Mouse. Host: Rabbit. Affinity purified rabbit polyclonal antibody More details

$495 / 0.1 mL +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Xin Actin-binding Repeat-containing Protein 1 (XIRP1) (Ser295), Polyclonal Antibody

Full Product Name

Anti-Phospho-Ser295 Xin Actin-Binding Repeat-Containing Protein1 (XIRP1)

Product Gene Name

anti-XIRP1 antibody

Clonality

Polyclonal

Host

Rabbit

Species Reactivity

Mouse

Form/Format

100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. Adequate amount of material to conduct 10- mini Western Blots.

Purity/Purification

Affinity Purified (Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.)

Specificity

Specific for the ~250k and ~150k splice variants of XIRP1 phosphorylated at Ser295. Immunolabeling of the XIRP1 band is blocked by the treatment of the lysate with lambda phosphatase (30 minutes, 800 units / 1 mg protein).

Preparation and Storage

Store at -20 degree C; stable for at least one year.

Molecular Weight

150, 250

3D Structure

ModBase 3D Structure for O70373

Antigen

Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser295 of mouse XIRP1.

Immunogen Information

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser295 conjugated to KLH

Immunogen Species

Mouse

Reactivity Assumed Based on 100% Sequence Homology

Canine, human, non-human primate, rat

Species Reactivity Note

The antibody has been directly tested for reactivity in Western blots with mouse tissue. It is anticipated that the antibody will react with canine, human, non-human primate, and rat tissues based on the fact that these species have 100% homology with the amino acid sequence used as antigen.

Biological Significance

XIRP1 is a member of the Xin family of proteins, containing 16 Xin repeats. The intercalated disk protein Xin protects actin filaments from depolymerization, and is able to bundle actin filaments to interact with beta-catenin, which is thought to stabilize actin-based cytoskeletons (Choi et al., 2007). The evolutionary emergence of the Xin paralogs may have played a key role in the development of heart chambers with complete endothelial and myocardial layers (Grosskurth et al., 2008). Loss of mXinalpha (mouse Xin homolog) results in cardiac hypertrophy and cardiomyopathy (Gustafson-Wagner et al., 2007). Phosphorylation of Ser295 is thought to play a key role in XIRP signaling.

Applications Tested/Suitable for anti-XIRP1 antibody

Western Blot (WB)

Application Notes for anti-XIRP1 antibody

Quality Control: Western blots performed on each lot.
WB: 1:1000

Product References and Citations for anti-XIRP1 antibody

• Choi S, Gustafson-Wagner EA, Wang Q, Harlan SM, Sinn HW, Lin J.L.-C., Lin, J.J.-C. The Intercalated Disc Protein, mXinalpha, Is Capable of Interacting with beta-Catenin and Bundling Actin Filaments. J Biol Chem. 2007; 282(49):36024-36036.
• Gustafson-Wagner EA, Sinn H.W., Chen Y.-L., Wang D.-Z., Reiter R.S., Lin J.L.-C., Yang B., Williamson R.A., Chen J., Lin C.-I., Lin J.J.-C. Loss of mXinalpha, an intercalated disk protein, results in cardiac hypertrophy and cardiomyopathy with conduction defects. (2007) Am J Physiol Heart Circ Physiol, November; 293(5): H2680-H2692.
• Grosskurth SE, Bhattacharya D, Wang Q, Lin J.J.-C. Emergence of Xin Demarcates a Key Innovation in Heart Evolution. (2008) PLoS One. Aug; 3(8):e2857

Other Notes

Small volumes of anti-XIRP1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for XIRP1. It may not necessarily be applicable to this product.

NCBI GI #

81870491

NCBI Accession #

O70373.2

UniProt Primary Accession #

O70373

NCBI Official Full Name

Xin actin-binding repeat-containing protein 1

UniProt Protein Name

Xin actin-binding repeat-containing protein 1

UniProt Synonym Protein Names

Cardiomyopathy-associated protein 1

Protein Family

Xin actin-binding repeat-containing protein

UniProt Gene Name

Xirp1

UniProt Synonym Gene Names

Cmya1; Xin

UniProt Entry Name

XIRP1_MOUSE

UniProt Comments for XIRP1

Function: Protects actin filaments from depolymerization

By similarity.

Subunit structure: Interacts with FLNC and VASP

By similarity. Interacts with F-actin and CTNNB1. Ref.6

Subcellular location: Cell junction

By similarity. Note: Colocalizes with actin stress fibers

By similarity.

Tissue specificity: Expressed in heart and skeletal muscle. In heart, present in intercalated disks (at protein level). Ref.1 Ref.5

Developmental stage: At E8, expression is restricted to cardiogenic cells. At E10, expressed in heart and rostral somites. At E13.5, expressed in heart and skeletal muscle (at protein level). Ref.4

Domain: Xin repeats bind F-actin.

Disruption phenotype: Mice are predisposed to hypertrophic cardiomyopathy, and display abnormal cardiac electrophysiological characteristics, including defects in atrial depolarization and ventricular repolarization. Ref.5

Sequence similarities: Belongs to the Xin family.Contains 17 Xin repeats.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-XIRP1 antibody

Reference Product	PubMed Publications
Flnc antibody	>3 publications with XIRP1 and Flnc
Ldb3 antibody	>1 publications with XIRP1 and Ldb3
Nrap antibody	>1 publications with XIRP1 and Nrap
Csrp3 antibody	>1 publications with XIRP1 and Csrp3

Organs/Tissues associated with anti-XIRP1 antibody

Organ/Tissue Name	Pubmed Publications
Heart Antibodies	>8 publications with XIRP1 and Heart
Embryonic Tissue Antibodies	>1 publications with XIRP1 and Embryonic Tissue