Rabbit Anti-MECP2 Polyclonal Antibody – Cat# MBS502181

MECP2 Antibody – Rabbit Polyclonal

Name: MECP2 Antibody; Polyclonal; Rabbit; Mouse-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS502181
Price: 495 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS502181

MBS502181 is an antibody targeting MECP2. Also known as Anti-Phospho-Ser421 Methyl-CpG Binding Protein 2 (MeCP2). Reactive in Mouse. Host: Rabbit. Rabbit polyclonal antibody More details

$495 / 0.1 mL +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

MeCP2 (Ser421), Polyclonal Antibody

Full Product Name

Anti-Phospho-Ser421 Methyl-CpG Binding Protein 2 (MeCP2)

Product Gene Name

anti-MECP2 antibody

Clonality

Polyclonal

Host

Rabbit

Species Reactivity

Mouse

Form/Format

100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol.. Adequate amount of material to conduct 10- mini Western Blots

Purity/Purification

Affinity Purified (Prepared from rabbit serum by affinity purification via sequential chromatography on phospho and dephosphopeptide affinity columns.)

Specificity

Specific for the ~55 kDa truncated MeCP2 protein phosphorylated at Ser421. Immunolabeling of the MeCP2 band is blocked by preadsorption with the phospho-peptide used as antigen but not by the corresponding dephospho-peptide.

Preparation and Storage

Store at -20 degree C in undiluted aliquots; stable for at least 1 year after date of receipt. Avoid freeze/thaw cycles.

Molecular Weight

52,307 Da

Chromosome Location

Chromosome: X; NC_000086.7 (74026592..74085636, complement). Location: X A7.3; X 37.63 cM

3D Structure

ModBase 3D Structure for Q9Z2D6

Antigen

Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser421 of MeCP2 (Methyl-CpG Binding Protein 2) protein.

Immunogen Information

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser421 conjugated to KLH

Immunogen Species

Mouse

Reactivity Assumed Based on 100% Sequence Homology

Human, non-human primate

Species Reactivity Note

The antibody has been directly tested for reactivity in Western blots with mouse tissue. It is anticipated that the antibody will react with human and non-human primate tissues based on the fact that these species have 100% homology with the amino acid sequence used as antigen.

Biological Significance

MeCP2 (Methyl-CpG Binding Protein 2) is a chromosomal protein that binds to methylated DNA. It can bind specifically to a single methyl-CpG pair and is not influenced by sequences flanking the methyl-CpGs. MeCP2 has been shown to mediate transcriptional repression through interaction with histone deacetylase and the corepressor SIN3A (Nan et al., 1998). Defects in MeCP2 are the cause of Rett syndrome (RTT) (Amir et al., 1999). RTT is an X-linked dominant disease; it is a progressive neurologic developmental disorder and one of the most common causes of mental retardation in females. Recent studies have reported a new phosphorylation site at Ser421. Phosphorylation and dephosphorylation of this site may be involved in regulation of behavioral responses to chronic antidepressant treatment (Hutchinson et al., 2012).

Applications Tested/Suitable for anti-MECP2 antibody

Western Blot (WB)

Application Notes for anti-MECP2 antibody

Quality Control: Western blots performed on each lot.
WB: 1:1000

Product References and Citations for anti-MECP2 antibody

• Amir RE, Van den Veyver IB, Wan M, Tran CQ, Francke U, Zoghbi HY (1999) Rett syndrome is caused by mutations in X-linked MECP2, encoding methyl-CpG-binding protein 2. Nat Genet. Oct;23(2):185-8.
• Hutchinson AN, Deng JV, Cohen S, West AE (2012) Phosphorylation of MeCP2 at Ser421 contributes to chronic antidepressant action. Journal of Neuroscience. Oct 10;32(41):14355-63.
• Nan X, Ng HH, Johnson CA, Laherty CD, Turner BM, Eisenman RN, Bird A (1998) Transcriptional repression by the methyl-CpG-binding protein MeCP2 involves a histone deacetylase complex. Nature. May 28;393(6683):386-9.

Other Notes

Small volumes of anti-MECP2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for MECP2. It may not necessarily be applicable to this product.

NCBI GI #

126517469

NCBI GeneID

17257

NCBI Accession #

NP_001075448.1

UniProt Primary Accession #

Q9Z2D6

UniProt Secondary Accession #

Q3TYG1; B1AUZ2; B1AUZ3

UniProt Related Accession #

Q9Z2D6

GenBank Nucleotide #

NM_001081979.1

NCBI Official Full Name

methyl-CpG-binding protein 2 isoform 1

NCBI Official Synonym Full Names

methyl CpG binding protein 2

NCBI Official Symbol

Mecp2

NCBI Official Synonym Symbols

Mbd5; WBP10; BB130002; 1500041B07Rik; D630021H01Rik

UniProt Protein Name

Methyl-CpG-binding protein 2

Protein Family

Methyl-CpG-binding protein

UniProt Gene Name

Mecp2

UniProt Synonym Gene Names

MeCp-2 protein; MeCp2

UniProt Entry Name

MECP2_MOUSE

NCBI Protein Information

methyl-CpG-binding protein 2; meCp-2 protein

UniProt Comments for MECP2

Function: Chromosomal protein that binds to methylated DNA. It can bind specifically to a single methyl-CpG pair. It is not influenced by sequences flanking the methyl-CpGs. Mediates transcriptional repression through interaction with histone deacetylase and the corepressor SIN3. Binds both 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC)-containing DNA, with a preference for 5-methylcytosine (5mC). Ref.10

Subunit structure: Interacts with CDKL5

By similarity. Interacts with FNBP3. Ref.7

Subcellular location: Nucleus. Note: Colocalized with methyl-CpG in the genome. Ref.8

Post-translational modification: Phosphorylated on Ser-421 by CaMK2 in brain upon synaptic activity, which attenuates its repressor activity and seems to regulate dendritic growth and spine maturation. Does not seem to be phosphorylated on Ser-421 in other tissues. Ref.9

Sequence similarities: Contains 2 A.T hook DNA-binding domains.Contains 1 MBD (methyl-CpG-binding) domain.

Research Articles on MECP2

1. Synaptic transmission is impaired in locus ceruleus neurons of Mecp2-null mice at a time when Rett-like symptoms manifest, evoking a potential mechanism for delayed onset of the disease.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Diseases associated with anti-MECP2 antibody

Disease Name	Pubmed Publications
Rett Syndrome Antibodies	>1099 publications with MECP2 and Rett Syndrome
Intellectual Disability Antibodies	>967 publications with MECP2 and Intellectual Disability
Disease Models, Animal Antibodies	>205 publications with MECP2 and Disease Models, Animal
Brain Diseases Antibodies	>152 publications with MECP2 and Brain Diseases
Seizures Antibodies	>135 publications with MECP2 and Seizures
Autistic Disorder Antibodies	>122 publications with MECP2 and Autistic Disorder
Child Development Disorders, Pervasive Antibodies	>86 publications with MECP2 and Child Development Disorders, Pervasive
Chromosome Aberrations Antibodies	>73 publications with MECP2 and Chromosome Aberrations
Angelman Syndrome Antibodies	>52 publications with MECP2 and Angelman Syndrome
Developmental Disabilities Antibodies	>37 publications with MECP2 and Developmental Disabilities

Organs/Tissues associated with anti-MECP2 antibody

Organ/Tissue Name	Pubmed Publications
Brain Antibodies	>674 publications with MECP2 and Brain
Embryonic Tissue Antibodies	>31 publications with MECP2 and Embryonic Tissue
Eye Antibodies	>22 publications with MECP2 and Eye
Kidney Antibodies	>12 publications with MECP2 and Kidney
Spleen Antibodies	>7 publications with MECP2 and Spleen
Mammary Gland Antibodies	>5 publications with MECP2 and Mammary Gland
Thymus Antibodies	>3 publications with MECP2 and Thymus