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COXIV (Ser58) (COX4i1) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS502194

MBS502194 is an antibody targeting COX4i1. Also known as Anti-Phospho-Ser58 COXIV. Reactive in Mouse, rat. Host: Rabbit. Affinity purified rabbit polyclonal antibody More details

$495 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
COXIV (Ser58) (COX4i1), Polyclonal Antibody
Full Product Name
Anti-Phospho-Ser58 COXIV
Product Gene Name
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Mouse, rat
Form/Format
100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug BSA per ml and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.
Purity/Purification
Affinity Purified (Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.)
Specificity
Specific for the ~17k COXIV-1 protein phosphorylated at Ser58. Immunolabeling is blocked by the phospho-peptide used as antigen but not by the corresponding dephospho-peptide.
Preparation and Storage
Store at -20 degree C; stable for at least one year.
Molecular Weight
17
Chromosome Location
Chromosome: 8; NC_000074.6 (120668230..120674209). Location: 8 E1; 8 69.99 cM
3D Structure
ModBase 3D Structure for P19783
Antigen
Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser58 of mouse COXIV protein.
Immunogen Information
Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser58 conjugated to KLH
Immunogen Species
Mouse
Species Reactivity Note
The antibody has been directly tested for reactivity in Western blots with mouse and rat tissue.
Biological Significance
COX, also known as cytochrome c oxidase, has 12 subunits that make up the transmembrane mitochondrial protein. Subunit IV has two isoforms; COXIV-1 and COXIV-2 (Huttermann et al., 2001). COXIV-1 is expressed ubiquitously while COXIV-2 is highly expressed in adult lung and low levels in brain and heart (Huttermann et al., 2001). Phosphorylation of amino acid residue ser58 of the COXIV-1 protein is a PKA-dependent regulation of COX and plays an important role in metabolism and CREB cycle activation (Acin-Perez et al., 2011).
Applications Tested/Suitable for anti-COX4i1 antibody
Western Blot (WB)
Application Notes for anti-COX4i1 antibody
Quality Control: Western blots performed on each lot.
WB: 1:1000

Testing Data of anti-COX4i1 antibody

Rabbit Anti-COX4i1 Polyclonal Antibody – Cat# MBS502194

Product References and Citations for anti-COX4i1 antibody

• Acin-Perez R, Gatti D, Bai Y, Manfredi D (2011) Protein Phosphorylation and Prevention of Cytochrome Oxidase Inhibition by ATP: Coupled Mechanisms of Energy Metabolism Regulation. Cell Metabolism 13, 712-719.
• Huttermann M, Kadenbach B, and Grossman LI (2001) Mammalian subunit IV isoforms of cytochrome c oxidase. Gene 267, 111-123.

Other Notes

Small volumes of anti-COX4i1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for COX4i1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
cytochrome c oxidase subunit 4 isoform 1, mitochondrial
NCBI Official Synonym Full Names
cytochrome c oxidase subunit IV isoform 1
NCBI Official Symbol
Cox4i1
NCBI Official Synonym Symbols
Cox4; COXIV; Cox4a; AL024441
UniProt Protein Name
Cytochrome c oxidase subunit 4 isoform 1, mitochondrial
UniProt Synonym Protein Names
Cytochrome c oxidase polypeptide IV; Cytochrome c oxidase subunit IV isoform 1
Protein Family
UniProt Gene Name
Cox4i1
UniProt Synonym Gene Names
Cox4; Cox4a; COX IV-1
UniProt Entry Name
COX41_MOUSE
NCBI Protein Information
cytochrome c oxidase subunit 4 isoform 1, mitochondrial; COX IV-1; cytochrome c oxidase, subunit IVa; cytochrome c oxidase polypeptide IV

UniProt Comments for COX4i1

Function: This protein is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport.

Subcellular location: Mitochondrion inner membrane.

Sequence similarities: Belongs to the cytochrome c oxidase IV family.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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