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Vesicular Monoamine Transporter 2, C-Terminus (VMAT2) Antibody – Sheep Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS502197

MBS502197 is an antibody targeting VMAT2. Also known as Anti-Vesicular Monoamine Transporter 2, C-Terminus. Reactive in Rat. Host: Sheep. Affinity purified sheep polyclonal antibody More details

$495 / 0.1 mL +1 FREE 8GB USB
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Sample Manual Insert View PDF
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Testing Data View PDF
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Product Overview

Online Datasheet in PDF
Product Name
Vesicular Monoamine Transporter 2, C-terminus (VMAT2), Polyclonal Antibody
Full Product Name
Anti-Vesicular Monoamine Transporter 2, C-Terminus
Product Gene Name
anti-VMAT2 antibody
Clonality
Polyclonal
Host
Sheep
Species Reactivity
Rat
Form/Format
100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western blots.
Purity/Purification
Affinity Purified (Prepared from sheep serum by affinity purification using a column to which the peptide immunogen was coupled.)
Specificity
Specific for the ~57k VMAT2 protein in Western blots of rat caudate lysate
Preparation and Storage
Store at -20 degree C in undiluted aliquots; stable for at least 1 year. Avoid freeze/thaw cycles.
Molecular Weight
57
Chromosome Location
Chromosome: 10; NC_000010.11 (117241073..117279430). Location: 10q25
OMIM
193001
3D Structure
ModBase 3D Structure for Q05940
Antigen
Peptide from intracellular C-terminal region of human VMAT2, conjugated to keyhole limpet hemocyanin (KLH).
Immunogen Information
Synthetic peptide corresponding to amino acid residues from the intracellular C-terminal region conjugated to KLH
Immunogen Species
Human
Species Reactivity Note
The antibody has been directly tested for reactivity in Western blots in rat tissue.
Biological Significance
Vesicular neurotransmitter transporters sequester the transmitters into synaptic vesicles (Erickson et al., 1996). The vesicular monoamine transporter 2 (VMAT2) is responsible for catecholamine and serotonin storage in central synapses. Antibodies specific for VMAT have been used to monitor expression of the transporter during development and in aging and can be effectively used as a marker for monoamine terminals (Haycock et al., 2003; Witkovsky et al., 2005).
Applications Tested/Suitable for anti-VMAT2 antibody
Western Blot (WB)
Application Notes for anti-VMAT2 antibody
Quality Control: Western blots performed on each lot.
WB: 1:1000

Testing Data of anti-VMAT2 antibody

Sheep Anti-VMAT2 Polyclonal Antibody – Cat# MBS502197

Product References and Citations for anti-VMAT2 antibody

• Erickson JD, Schafer MKH, Bonner TI, Eiden LE, Weihe E (1996) Distinct pharmacological properties and distribution in neurons and endocrine cells of two isoforms of the human vesicular monoamine transporter. Proc Natl Acad Sci USA 93:5166-5171.
• Giros B, El Mestikawy S, Godinot N, Zheng K, Han H, Yang-Feng T, Caron MG (1991) Cloning, pharmacological characterization, and chromosome assignment of the human dopamine transporter. J Pharmacol Exptl Ther 56:2139-2142
• Haycock JW, Becker L, Ang, L, Furukawa Y, Hornykiewicz O, Kish SJ (2003) Marked disparity between age-related changes in dopamine and other presynaptic dopaminergic markers in human striatum. J Neurochem 87:574-585.
• Witkovsky P, Arango-Gonzalez B, Haycock JW, Kohler K (2004) Rat retinal dopaminergic neurons: Differential maturation of somatodendritic and axonal compartments. J Comp Neurol 481:352-362.

Other Notes

Small volumes of anti-VMAT2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for VMAT2. It may not necessarily be applicable to this product.

NCBI GI #
42476325
NCBI GeneID
6571
NCBI Accession #
NP_003045.2
UniProt Primary Accession #
Q05940
UniProt Secondary Accession #
Q15876; Q5VW49; Q9H3P6; B2RC96; D3DRC4
UniProt Related Accession #
Q05940
GenBank Nucleotide #
NM_003054.4
NCBI Official Full Name
synaptic vesicular amine transporter
NCBI Official Synonym Full Names
solute carrier family 18 (vesicular monoamine transporter), member 2
NCBI Official Symbol
SLC18A2
NCBI Official Synonym Symbols
SVAT; SVMT; VAT2; VMAT2
UniProt Protein Name
Synaptic vesicular amine transporter
UniProt Synonym Protein Names
Monoamine transporter; Solute carrier family 18 member 2; Vesicular amine transporter 2
UniProt Gene Name
SLC18A2
UniProt Synonym Gene Names
SVMT; VMAT2; VAT2
UniProt Entry Name
VMAT2_HUMAN
NCBI Protein Information
synaptic vesicular amine transporter; monoamine transporter; vesicular amine transporter 2; vesicle monoamine/H+ antiporter; solute carrier family 18 member 2; vesicle monoamine transporter type 2; monoamine neurotransmitter transporter; solute carrier family 18 (vesicular monoamine), member 2

NCBI Summary for VMAT2

The vesicular monoamine transporter acts to accumulate cytosolic monoamines into synaptic vesicles, using the proton gradient maintained across the synaptic vesicular membrane. Its proper function is essential to the correct activity of the monoaminergic systems that have been implicated in several human neuropsychiatric disorders. The transporter is a site of action of important drugs, including reserpine and tetrabenazine (summary by Peter et al., 1993 [PubMed 7905859]). See also SLC18A1 (MIM 193002).[supplied by OMIM, Jan 2011]
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UniProt Comments for VMAT2

Function: Involved in the ATP-dependent vesicular transport of biogenic amine neurotransmitters. Pumps cytosolic monoamines including dopamine, norepinephrine, serotonin, and histamine into synaptic vesicles. Requisite for vesicular amine storage prior to secretion via exocytosis.

Subunit structure: Interacts with SLC6A3

By similarity.

Subcellular location: Cytoplasmic vesicle membrane; Multi-pass membrane protein.

Sequence similarities: Belongs to the major facilitator superfamily. Vesicular transporter family.

Sequence caution: The sequence BAB19009.1 differs from that shown. Reason: Erroneous gene model prediction.
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Research Articles on VMAT2

1. Genetic variation in VMAT2 may be linked to alterations in cognitive functioning underlying psychotic disorder, possibly through altered transport of monoamines into synaptic vesicles.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-VMAT2 antibody

 Reference Product  PubMed Publications
 SLC6A3 antibody  >34 publications with VMAT2 and SLC6A3
 PACS1 antibody  >1 publications with VMAT2 and PACS1
 SLC22A2 antibody  >1 publications with VMAT2 and SLC22A2

Pathways associated with anti-VMAT2 antibody

 Products by Pathway  Pathway Diagram
 Alcoholism Pathway antibodies  Alcoholism Pathway Diagram
 Alcoholism Pathway antibodies  Alcoholism Pathway Diagram
 Amphetamine Addiction Pathway antibodies  Amphetamine Addiction Pathway Diagram
 Amphetamine Addiction Pathway antibodies  Amphetamine Addiction Pathway Diagram
 Cocaine Addiction Pathway antibodies  Cocaine Addiction Pathway Diagram
 Cocaine Addiction Pathway antibodies  Cocaine Addiction Pathway Diagram
 Dopamine Neurotransmitter Release Cycle Pathway antibodies  Dopamine Neurotransmitter Release Cycle Pathway Diagram
 Dopaminergic Synapse Pathway antibodies  Dopaminergic Synapse Pathway Diagram
 Dopaminergic Synapse Pathway antibodies  Dopaminergic Synapse Pathway Diagram
 Na+/Cl- Dependent Neurotransmitter Transporters Pathway antibodies  Na+/Cl- Dependent Neurotransmitter Transporters Pathway Diagram

Diseases associated with anti-VMAT2 antibody

 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >105 publications with VMAT2 and Nervous System Diseases
 Brain Diseases Antibodies  >89 publications with VMAT2 and Brain Diseases
 Movement Disorders Antibodies  >83 publications with VMAT2 and Movement Disorders
 Parkinsonian Disorders Antibodies  >67 publications with VMAT2 and Parkinsonian Disorders
 Disease Models, Animal Antibodies  >48 publications with VMAT2 and Disease Models, Animal
 Parkinson Disease Antibodies  >46 publications with VMAT2 and Parkinson Disease
 Neurotoxicity Syndromes Antibodies  >19 publications with VMAT2 and Neurotoxicity Syndromes
 Poisoning Antibodies  >19 publications with VMAT2 and Poisoning
 Nerve Degeneration Antibodies  >19 publications with VMAT2 and Nerve Degeneration
 Parkinson Disease, Secondary Antibodies  >13 publications with VMAT2 and Parkinson Disease, Secondary

Organs/Tissues associated with anti-VMAT2 antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >276 publications with VMAT2 and Brain
 Blood Antibodies  >51 publications with VMAT2 and Blood
 Pancreas Antibodies  >20 publications with VMAT2 and Pancreas
 Stomach Antibodies  >17 publications with VMAT2 and Stomach
 Adrenal Gland Antibodies  >11 publications with VMAT2 and Adrenal Gland
 Embryonic Tissue Antibodies  >8 publications with VMAT2 and Embryonic Tissue
 Eye Antibodies  >7 publications with VMAT2 and Eye
 Skin Antibodies  >5 publications with VMAT2 and Skin
 Kidney Antibodies  >5 publications with VMAT2 and Kidney
 Lung Antibodies  >4 publications with VMAT2 and Lung
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