Rabbit Anti-PTPH1 Polyclonal Antibody – Cat# MBS502201

PTPH1 Antibody – Rabbit Polyclonal

Name: PTPH1 Antibody; Polyclonal; Rabbit; Human/Mouse-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS502201
Price: 495 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS502201

Affinity purified rabbit polyclonal antibodyProtein-tyrosine Phosphatase H1 (PTPH1) has recently been identified as a specific p38gamma MAPK phosphatase which is mediated through PDZ interaction (Hou et al., 2010). Ras has been demonstrated to increase both… More details

$495 / 0.1 mL +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

PTPH1 (Ser459), Polyclonal Antibody

Full Product Name

Anti-Phospho-Ser459 Protein Tyrosine Phosphatase H1 (PTPH1)

Product Gene Name

anti-PTPH1 antibody

Clonality

Polyclonal

Host

Rabbit

Species Reactivity

Human, mouse

Form/Format

100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

Purity/Purification

Affinity Purified (Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.)

Specificity

Specific for ~104k PTPH1 protein phosphorylated at Ser459. Immunolabeling is blocked by preadsorption of the antibody with the phosphopeptide used as antigen but not by the corresponding dephosphopeptide. Immunolabeling is also completely eliminated by treatment with lambda phosphatase.

Preparation and Storage

For long term storage -20 degree C is recommended. Stable at -20 degree C for at least 1 year.

Molecular Weight

104

Chromosome Location

Chromosome: 9; NC_000009.12 (109375694..109498320, complement). Location: 9q31

OMIM

176877

3D Structure

ModBase 3D Structure for P26045

Antigen

Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser459 of human PTPH1.

Immunogen Information

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser459 conjugated to KLH

Immunogen Species

Human

Reactivity Assumed Based on 100% Sequence Homology

Bovine, canine, non-human primate, sheep

Species Reactivity Note

The antibody has been directly tested for reactivity in Western blots with human and mouse tissue. It is anticipated that the antibody will react with bovine, canine, non-human primates and sheep based on the fact that these species have 100% homology with the amino acid sequence used as antigen.

Applications Tested/Suitable for anti-PTPH1 antibody

Western Blot (WB)

Application Notes for anti-PTPH1 antibody

Quality Control: Western blots performed on each lot.
WB: 1:1000

Product References and Citations for anti-PTPH1 antibody

• Hou SW, Zhi HY, Pohl N, Loesch M, Qi XM, Li RS, Basir Z, Chen G (2010) PTPH1 Dephosphorylates and Cooperates with p38gamma MAPK to Increase Ras Oncogenesis through PDZ-Mediated Interation. Cancer Res 70:2901-2910.
• Hou SW, Suresh PS, Qi X, Lepp A, Mirza SP, Chen G (2012) p38gamma Mitogen-activated Protein Kinase Signals through Phosphorylating Its Phosphatase PTPH1 in Regulating Ras Protein Oncogenesis and Stress Response. J Biol Chem 287(33): 27895-905.

Other Notes

Small volumes of anti-PTPH1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PTPH1 purchase

MBS502201 is an antibody targeting PTPH1. Also known as Anti-Phospho-Ser459 Protein Tyrosine Phosphatase H1 (PTPH1). Reactive in Human, mouse. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for PTPH1. It may not necessarily be applicable to this product.

NCBI GI #

223941879

NCBI GeneID

5774

NCBI Accession #

NP_001138840.1

UniProt Primary Accession #

P26045

UniProt Secondary Accession #

A0AUW9; E7EN99; E9PGU7

UniProt Related Accession #

P26045

GenBank Nucleotide #

NM_001145368.1

NCBI Official Full Name

tyrosine-protein phosphatase non-receptor type 3 isoform 2

NCBI Official Synonym Full Names

protein tyrosine phosphatase, non-receptor type 3

NCBI Official Symbol

PTPN3

NCBI Official Synonym Symbols

PTPH1; PTP-H1

UniProt Protein Name

Tyrosine-protein phosphatase non-receptor type 3

UniProt Synonym Protein Names

Protein-tyrosine phosphatase H1

UniProt Gene Name

PTPN3

UniProt Synonym Gene Names

PTPH1; PTP-H1

UniProt Entry Name

PTN3_HUMAN

NCBI Protein Information

tyrosine-protein phosphatase non-receptor type 3; protein-tyrosine phosphatase H1; cytoskeletal-associated protein tyrosine phosphatase

NCBI Summary for PTPH1

The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This protein contains a C-terminal PTP domain and an N-terminal domain homologous to the band 4.1 superfamily of cytoskeletal-associated proteins. P97, a cell cycle regulator involved in a variety of membrane related functions, has been shown to be a substrate of this PTP. This PTP was also found to interact with, and be regulated by adaptor protein 14-3-3 beta. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2009]

UniProt Comments for PTPH1

Function: May act at junctions between the membrane and the cytoskeleton. Possesses tyrosine phosphatase activity.

Catalytic activity: Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.

Subcellular location: Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasm › cytoskeleton.

Sequence similarities: Belongs to the protein-tyrosine phosphatase family. Non-receptor class subfamily.Contains 1 FERM domain.Contains 1 PDZ (DHR) domain.Contains 1 tyrosine-protein phosphatase domain.

Research Articles on PTPH1

1. p38gamma Mitogen-activated protein kinase signals through phosphorylating its phosphatase PTPH1 in regulating ras protein oncogenesis and stress response.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-PTPH1 antibody

Reference Product	PubMed Publications
SRC antibody	>3 publications with PTPH1 and SRC

Diseases associated with anti-PTPH1 antibody

Disease Name	Pubmed Publications
Carcinoma, Hepatocellular Antibodies	>2 publications with PTPH1 and Carcinoma, Hepatocellular
Breast Neoplasms Antibodies	>2 publications with PTPH1 and Breast Neoplasms
Liver Diseases Antibodies	>2 publications with PTPH1 and Liver Diseases
Lung Diseases Antibodies	>1 publications with PTPH1 and Lung Diseases
Hepatitis Antibodies	>1 publications with PTPH1 and Hepatitis
Neoplasms, Experimental Antibodies	>1 publications with PTPH1 and Neoplasms, Experimental
Necrosis Antibodies	>1 publications with PTPH1 and Necrosis
Inflammation Antibodies	>1 publications with PTPH1 and Inflammation

Organs/Tissues associated with anti-PTPH1 antibody

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>5 publications with PTPH1 and Blood
Liver Antibodies	>4 publications with PTPH1 and Liver
Muscle Antibodies	>3 publications with PTPH1 and Muscle
Lung Antibodies	>2 publications with PTPH1 and Lung
Connective Tissue Antibodies	>1 publications with PTPH1 and Connective Tissue
Nerve Antibodies	>1 publications with PTPH1 and Nerve
Bone Antibodies	>1 publications with PTPH1 and Bone
Intestine Antibodies	>1 publications with PTPH1 and Intestine
Brain Antibodies	>1 publications with PTPH1 and Brain