Rabbit Anti-KCC2 Polyclonal Antibody – Cat# MBS502206

Potassium Chloride Cotransporter (KCC2) (Ser940) (KCC2) Antibody – Rabbit Polyclonal

Name: KCC2 Antibody; Polyclonal; Rabbit; Bovine/Canine/Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS502206
Price: 495 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS502206

MBS502206 is an antibody targeting KCC2. Also known as Anti-Phospho-Ser940 Potassium Chloride Cotransporter (KCC2). Reactive in Bovine, Canine, Human, Non-Human Primate, Zebrafish. Host: Rabbit. Affinity purified rabbit polyclonal antibody More details

$495 / 0.1 mL +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Potassium Chloride Cotransporter (KCC2) (Ser940), Polyclonal Antibody

Full Product Name

Anti-Phospho-Ser940 Potassium Chloride Cotransporter (KCC2)

Product Gene Name

anti-KCC2 antibody

Clonality

Polyclonal

Host

Rabbit

Species Reactivity

Bovine, Canine, Human, Non-Human Primate, Zebrafish

Form/Format

100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

Purity/Purification

Antigen Affinity Purified from Pooled Serum
(Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.)

Specificity

Specific for the ~135k KCC2 protein phosphorylated at Ser940. Immunolabeling of the KCC2 protein band is blocked by the phospho-peptide used as antigen but not by the corresponding dephosphopeptide.

Preparation and Storage

For long term storage -80°C is recommended, but shorter term storage at -20°C is also acceptable as aliquots may be taken without without freeze/thawing due to the presence of 50% glycerol. Stock solutions for a minimum of 1 year at -20°C.

Molecular Weight

135kDa

Chromosome Location

Chromosome: 3; NC_005102.3 (167617776..167649551). Location: 3q42

3D Structure

ModBase 3D Structure for Q63633

Species Tested

Mouse, Rat

Immunogen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser940 of rat KCC2, conjugated to keyhole limpet hemocyanin (KLH).

Biological Significance

KCC2 is widely thought to be expressed exclusively in neurons where it is responsible for maintaining low intracellular chloride concentration to drive hyperpolarizing post-synaptic responses to the inhibitory neurotransmitters GABA and glycine. Serine 940 on KCC2 has been shown to be phosphorylated by PKC and has further been demonstrated to be the major site for PKC-dependent phosphorylation for full length KCC2 molecules when expressed in HEK-293 cells as phosphorylation of Ser940 increased the cell surface stability of KCC2 in this system by decreasing it's rate of internalization from the plasma membrane (Lee et al., 2007).

Applications Tested/Suitable for anti-KCC2 antibody

Western Blot (WB)

Application Notes for anti-KCC2 antibody

Quality Control: Western blots performed on each lot.
WB: 1:1000

Product References and Citations for anti-KCC2 antibody

• Lee HH, Walker JA, Williams JR, Goodier RJ, Payne JA, Moss SJ (2007) Direct protein kinase C-dependent phosphorylation regulates the cell surface stability and activity of the potassium chloride cotransporter KCC2. J Biol Chem. 2007 Oct 12;282(41):29777-84
• Lee HH, Deeb TZ, Walker JA, Davies PA, Moss SJ (2011) NMDA receptor activity downregulates KCC2 resulting in depolarizing GABAA receptor-mediated currents. Nat Neurosci. Jun;14(6):736-43

Other Notes

Small volumes of anti-KCC2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for KCC2. It may not necessarily be applicable to this product.

NCBI GI #

19705463

NCBI GeneID

171373

NCBI Accession #

NP_599190.1

UniProt Primary Accession #

Q63633

UniProt Secondary Accession #

A7Y821

UniProt Related Accession #

Q63633

GenBank Nucleotide #

NM_134363.1

NCBI Official Full Name

solute carrier family 12 member 5

NCBI Official Synonym Full Names

solute carrier family 12 (potassium-chloride transporter), member 5

NCBI Official Symbol

Slc12a5

NCBI Official Synonym Symbols

Kcc2

UniProt Protein Name

Solute carrier family 12 member 5

UniProt Synonym Protein Names

Electroneutral potassium-chloride cotransporter 2; Furosemide-sensitive K-Cl cotransporter; K-Cl cotransporter 2; rKCC2; Neuronal K-Cl cotransporter

UniProt Gene Name

Slc12a5

UniProt Synonym Gene Names

Kcc2; rKCC2

UniProt Entry Name

S12A5_RAT

NCBI Protein Information

solute carrier family 12 member 5; rKCC2; K-Cl cotransporter 2; neuronal K-Cl cotransporter; solute carrier family 12, member 5; neuronal-specific K-Cl cotransporter; furosemide-sensitive K-Cl cotransporter; electroneutral potassium-chloride cotransporter 2

NCBI Summary for KCC2

neuronal specific potassium-chloride cotransporter isoform; may play a role in response to neuronal injury [RGD, Feb 2006]

UniProt Comments for KCC2

Function: Mediates electroneutral potassium-chloride cotransport in mature neurons. Transport occurs under isotonic conditions, but is activated 20-fold by cell swelling. Important for Cl- homeostasis in neurons. Necessary for the ontogenic change in response to gamma-aminobutyric acid (GABA) from depolarization to hyperpolarization during neuronal development. Ref.3

Enzyme regulation: Inhibited by WNK3

By similarity.

Subunit structure: Homomultimer and heteromultimer with other K-Cl cotransporters. Interacts with AP2A1

By similarity. Ref.4

Subcellular location: Membrane; Multi-pass membrane protein.

Tissue specificity: Highly expressed in brain. Not detected in other tissues. Highly expressed in pyramidal neurons and in neurons throughout the cortex, hippocampus, the granular layer of the cerebellum and in groups of neurons throughout the brainstem. Barely detectable in dorsal-root ganglions.

Developmental stage: Detected in thalamus, but not in hippocampus and neocortex at E20. At birth barely detectable in hippocampus. Expression increases steeply from day 5 to day 9 and then stabilizes at adult levels.

Sequence similarities: Belongs to the SLC12A transporter family.

Research Articles on KCC2

1. KCC2 is part of a cascade that regulates activation of the descending pain facilitation.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-KCC2 antibody

Products by Pathway	Pathway Diagram
Cation-coupled Chloride Cotransporters Pathway antibodies	Cation-coupled Chloride Cotransporters Pathway Diagram
GABAergic Synapse Pathway antibodies	GABAergic Synapse Pathway Diagram
GABAergic Synapse Pathway antibodies	GABAergic Synapse Pathway Diagram
SLC-mediated Transmembrane Transport Pathway antibodies	SLC-mediated Transmembrane Transport Pathway Diagram
Transmembrane Transport Of Small Molecules Pathway antibodies	Transmembrane Transport Of Small Molecules Pathway Diagram
Transport Of Inorganic Cations/anions And Amino Acids/oligopeptides Pathway antibodies	Transport Of Inorganic Cations/anions And Amino Acids/oligopeptides Pathway Diagram

Diseases associated with anti-KCC2 antibody

Disease Name	Pubmed Publications
Growth Disorders Antibodies	>2 publications with KCC2 and Growth Disorders
Cognition Disorders Antibodies	>2 publications with KCC2 and Cognition Disorders
Epilepsy Antibodies	>2 publications with KCC2 and Epilepsy
Neoplasms Antibodies	>1 publications with KCC2 and Neoplasms
Hypertension Antibodies	>1 publications with KCC2 and Hypertension
Necrosis Antibodies	>1 publications with KCC2 and Necrosis
Liver Diseases Antibodies	>1 publications with KCC2 and Liver Diseases
Kidney Diseases Antibodies	>1 publications with KCC2 and Kidney Diseases
Seizures Antibodies	>1 publications with KCC2 and Seizures

Organs/Tissues associated with anti-KCC2 antibody

Organ/Tissue Name	Pubmed Publications
Brain Antibodies	>8 publications with KCC2 and Brain