Beclin1 (Ser295) (BECN1) Antibody – Rabbit Polyclonal

Function: Plays a central role in autophagy. Required for the abcission step in cytokinesis. May play a role in antiviral host defense. Protects against infection by a neurovirulent strain of Sindbis virus. Ref.13

Subunit structure: Interacts with GOPC and GRID2. Interacts with AMBRA1. Forms a complex with PIK3C3 and PIK3R4 and either UVRAG and KIAA0226/Rubicon, or with ATG14. UVRAG and ATG14 form mutually exclusive complexes through direct competition for BECN1-binding. The complex containing ATG14 up-regulates autophagy, while the one containing Rubicon down-regulates autophagy

By similarity. Interacts with BCL2 and BCL2L1. Interacts with VMP1. Interacts with PIK3CB

By similarity. Interacts with DAPK1. Interacts with human cytomegalovirus/HHV-5 protein TRS1. Ref.1 Ref.6 Ref.8 Ref.9 Ref.10 Ref.12 Ref.14 Ref.16

Subcellular location: Golgi apparatus › trans-Golgi network membrane; Peripheral membrane protein. Note: Interaction with ATG14 promotes translocation to autophagosomes. Expressed in dendrites and cell bodies of cerebellar Purkinje cells

By similarity. Ref.9

Tissue specificity: Ubiquitous.

Post-translational modification: Phosphorylation at Thr-119 by DAPK1 reduces its interaction with BCL2 and BCL2L1 and promotes induction of autophagy.polyubiquitinated by NEDD4, both with 'Lys11'- and 'Lys63'-linkages. 'Lys'-11-linked poyubiquitination leads to degradation and is enhanced when the stabilizing interaction partner VPS34 is depleted. Deubiquitinated by USP10 and USP13, leading to stabilize the PIK3C3/VPS34-containing complexes.

Sequence similarities: Belongs to the beclin family.