Troponin I (Cardiac) (TNNI3) Antibody – Rabbit Polyclonal

Polyclonal

Rabbit

Mouse, rat

100 ul neat serum. Adequate amount of material to conduct 10-mini Western blots.

Neat Serum (Unpurified neat serum.)

Specific for the ~25k cardiac troponin I protein.

Store at -20 degree C; stable for at least 1 year

25

Chromosome: 7; NC_000073.6 (4518308..4522443, complement). Location: 7 A1; 7 2.61 cM

ModBase 3D Structure for P48787

Fusion protein of the mouse cardiac troponin I holoprotein.

Fusion protein of complete mouse cardiac troponin 1

Mouse

The antibody has been directly tested for reactivity in Western blots with mouse and rat cardiac tissue.

Troponin I (cTnI) is 1 of 3 subunits, along with troponin C (TnC) and troponin T (TnT) of troponin complex found in cardiac muscle. cTnI binds to actin in thin myofilaments to hold the troponin-tropomyosin complex in place. Phosphorylation of cardiac isoform of TnI at serines 22,23 in the unique amino-terminal end molecule decreases the calcium sensitivity of the sarcomere, promotes calcium dissociation from troponin C and by extension enhances rates of cross-bridge cycling and diastolic relaxation (Noland, Jr. et al., 1995; Noland et al., 1989). In addition, studies using reconstituted fibers and mutational analysis have shown that PKC phosphorylation of TnI (largely at Ser43) inhibits the actin-cross bridge reaction and reduces the Ca++ dependent actomyosin ATPase rate as well as the calcium sensitivity of force generation (Noland, Jr. and Kuo, 1991). Phosphorylation at Thr144 (mediated by several PKC isoforms) reduces maximal tension development and cross-bridge cycling rates (Sumandea et al., 2008). Importantly, changes in the phosphorylation at each of these sites have been shown to be stage-specific with regard to cardiac disease progression (Walker et al., 2010).