Thr276 Serotonin Transporter (SLC6A4) Antibody – Rabbit Polyclonal

Polyclonal

Rabbit

Rat, Bovine, Human, Mouse, Non-Human Primate, Sheep, Xenopus

100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol.

Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephospho-peptide affinity columns.

Specific for the ~76k SERT protein phosphorylated at Ser276. Immunolabeling is blocked by preadsorption of antibody with the phospho-peptide that was used to generate the antibody but not by the corresponding dephospho-peptide.

Store at -20 degree C.

~ 76 kDa

ModBase 3D Structure for P31652

Rat

Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr276 conjugated to KLH

The serotonin transporter (SERT) recycles serotonin by transporting it back to the pre-synaptic cell. It is the primary target for most anti-depressant drugs and for stimulants such as methamphetamines. SERT is regulated by several processes, including a cyclic GMP signaling pathway involving nitric oxide synthase, guanylyl cyclase, and cGMP-dependent protein kinase (PKG). cGMP- and PKG-mediated SERT regulation requires phosphorylation at thr276 (Ramamoorthy et al., 2007). It has been suggested that although PKG is involved in the stimulation of SERT at thr276, it does not directly phosphorylate the residue, rather it initiates a kinase cascade that leads to SERT phosphorylation by an as yet unidentified protein kinase (Wong et al., 2012). Also of therapeutic importance, mutation at the thr276 residue has been shown to decrease the potency of a variety of anti-depressant drugs, (Zhang YW and Rudnick G, 2005).

Phosphopeptide corresponding to amino acid residues surrounding the phospho-Thr276 of rat SERT.