Rabbit Anti-DENND3 Polyclonal Antibody – Cat# MBS502222

Western blot of HeLa lysate showing specific immunolabeling of the ~142kD DENND3 protein phosphorylated at Ser554 (control). Phosphospecificity is shown in the second lane (lambda-phosphatase: lambda-Ptase). The blot is identical to the control except that the lysate was incubated in lambda-Ptase (800 units/1mg protein for 30 min). The immunolabeling is completely eliminated by treatment with lambda-Ptase.

Ser554 DENND3 (DENND3) Antibody – Rabbit Polyclonal

Name: DENND3 Antibody; Polyclonal; Rabbit; Human/Mouse/Rat-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS502222
Price: 495 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS502222

MBS502222 is an antibody targeting DENND3. Also known as Anti-Phospho-Ser554 DENND3. Reactive in Human, Mouse, Non-Human Primate, Rat, Sheep. Host: Rabbit. More details

$495 / 0.1 mL +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Ser554 DENND3, Polyclonal Antibody

Full Product Name

Anti-Phospho-Ser554 DENND3

Product Gene Name

anti-DENND3 antibody

Product Synonym Names

DENND3 (Ser554)

Clonality

Polyclonal

Host

Rabbit

Species Reactivity

Human, Mouse, Non-Human Primate, Rat, Sheep

Form/Format

100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. Adequate amount of material to conduct 10 mini western blots

Purity/Purification

Affinity purified rabbit polyclonal antibody

Specificity

Specific for the ~142kD DENND3 phosphorylated at Ser⁵⁵⁴. Immunolabeling is blocked by lambda-phosphate treatment

Preparation and Storage

Store at -20 degree C; stable for one year

Molecular Weight

~142 kDa

3D Structure

ModBase 3D Structure for D4A2H4

Quality Control Tests

Western Blot performed on each lot

Biological Significance

The DENN (differentially expressed in normal and neoplastic cells) domain (DENND) is a poorly characterized protein module conserved throughout evolution (McPherson et al., 2011). Proteins bearing a DENN domain have recently emerged as the largest family of Rab GEFs. Among these DENN domain proteins, DENND3 is a GEF for Rab12; promoting the exchange of GDP to GTP, converting inactive GDP-bound RAB12 into its active GTP-bound form (McPherson and Xu, 2015; Matsui et al., 2011). ULK-mediated phosphorylation of DENND3 at serines 554 and 572 upregulates its GEF activity toward the small GTPase Rab12 (McPherson and Xu, 2015).

Applications Tested/Suitable for anti-DENND3 antibody

Western Blot (WB)

Application Notes for anti-DENND3 antibody

WB: 1:500

Other Notes

Small volumes of anti-DENND3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for DENND3. It may not necessarily be applicable to this product.

UniProt Primary Accession #

D4A2H4

UniProt Protein Name

Protein Dennd3

UniProt Synonym Protein Names

Protein Dennd3

Protein Family

DENN domain-containing protein

UniProt Gene Name

Dennd3

UniProt Entry Name

D4A2H4_RAT

UniProt Comments for DENND3

DENND3: a guanine nucleotide exchange factor (GEF) activating RAB12. Promotes the exchange of GDP to GTP, converting inactive GDP-bound RAB12 into its active GTP-bound form. Plays a role in protein transport from recycling endosomes to lysosomes regulating, for example, the degradation of the transferrin receptor. Four isoforms of the human protein are produced by alternative splicing.

Protein type: GEFs; GEFs, Rab

Cellular Component: intracellular

Molecular Function: Rab guanyl-nucleotide exchange factor activity

Biological Process: cellular protein catabolic process; endosome to lysosome transport; positive regulation of GTPase activity

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.