Rabbit Anti-TFAM Polyclonal Antibody – Cat# MBS502223

Western blot of HeLa lysate showing specific immunolabeling of the ~24k TFAM protein.

TFAM Antibody – Rabbit Polyclonal

Name: TFAM Antibody; Polyclonal; Rabbit; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS502223
Price: 435 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS502223

MBS502223 is an antibody targeting TFAM. Also known as Anti-TFAM (Transcription Factor A, mitochondrial). Reactive in Human. Host: Rabbit. More details

$435 / 0.1 mL +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

TFAM, Polyclonal Antibody

Full Product Name

Anti-TFAM (Transcription Factor A, mitochondrial)

Product Gene Name

anti-TFAM antibody

Product Synonym Names

TFAM (Transcription Factor A, motochondrial)

Clonality

Polyclonal

Host

Rabbit

Species Reactivity

Human

Form/Format

100 ul serum

Purity/Purification

Neat Serum

Specificity

Specific for the ~24 kDa TFAM protein.

Preparation and Storage

Store at -20 degree C.

Molecular Weight

~24 kDa

OMIM

600438

3D Structure

ModBase 3D Structure for Q00059

Immunogen Species

Human

Immunogen Info

Native recombinant human TFAM protein with c-terminal 6-his tag

Biological Significance

Mitochondrial Transcription Factor A (TFAM) is a key activator of mitochondrial (mt) DNA transcription as well as a participant in mitochondrial genome replication. mtDNA is highly susceptible to oxidative stress leading to mitochondrial dysfunction. Overexpression of TFAM has been implicated in the amelioration of age dependent impairment of brain functions through the prevention of oxidative stress and mitochondrial dysfunction in microglia (Hayashi et al., 2008). More recently, TFAM overexpression has been shown to potentially reduce oxidative stress in motor neurons and delay onset of amyotrophic lateral sclerosis (ALS) in ALS model mice (Morimoto et al., 2012).

Antigen

Native recombinant human TFAM protein with c-terminal 6-his tag

Applications Tested/Suitable for anti-TFAM antibody

Western Blot (WB)

Application Notes for anti-TFAM antibody

WB: 1:2000

Other Notes

Small volumes of anti-TFAM antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for TFAM. It may not necessarily be applicable to this product.

NCBI GI #

399498566

NCBI GeneID

7019

NCBI Accession #

NP_001257711.1

UniProt Primary Accession #

Q00059

UniProt Secondary Accession #

Q5U0C6; A8MRB2; A9QXC6; B5BU05

UniProt Related Accession #

Q00059

GenBank Nucleotide #

NM_001270782.1

NCBI Official Full Name

transcription factor A, mitochondrial isoform 2

NCBI Official Synonym Full Names

transcription factor A, mitochondrial

NCBI Official Symbol

TFAM

NCBI Official Synonym Symbols

TCF6; MTTF1; MTTFA; TCF6L1; TCF6L2; TCF6L3

UniProt Protein Name

Transcription factor A, mitochondrial

UniProt Synonym Protein Names

Mitochondrial transcription factor 1; MtTF1; Transcription factor 6; TCF-6; Transcription factor 6-like 2

Protein Family

Transcription factor

UniProt Gene Name

TFAM

UniProt Synonym Gene Names

TCF6; TCF6L2; mtTFA; MtTF1; TCF-6

UniProt Entry Name

TFAM_HUMAN

NCBI Protein Information

transcription factor A, mitochondrial

NCBI Summary for TFAM

This gene encodes a key mitochondrial transcription factor containing two high mobility group motifs. The encoded protein also functions in mitochondrial DNA replication and repair. Sequence polymorphisms in this gene are associated with Alzheimer's and Parkinson's diseases. There are pseudogenes for this gene on chromosomes 6, 7, and 11. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2012]

UniProt Comments for TFAM

TFAM: regulates mitochondrial transcription. Induced in humans by caloric restriction. Required for accurate and efficient promoter recognition by the mitochondrial RNA polymerase. Activates transcription by binding immediately upstream of transcriptional start sites. Is able to unwind and bend DNA. Involved in cisplatin resistance. Interacts with TFB1M and TFB2M.

Protein type: Transcription regulation; DNA-binding

Chromosomal Location of Human Ortholog: 10q21

Cellular Component: cytosol; mitochondrial matrix; mitochondrion; nucleus

Molecular Function: chromatin binding; DNA bending activity; heat shock protein binding; protein binding; transcription factor activity

Biological Process: DNA-dependent DNA replication; mitochondrial respiratory chain complex assembly; mitochondrion organization and biogenesis; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; regulation of transcription from RNA polymerase I promoter; transcription from mitochondrial promoter; transcription from RNA polymerase II promoter; transcription initiation from mitochondrial promoter

Research Articles on TFAM

1. TFAM overexpression suppressed mitoROS and their upregulation in rat cardiomyocytes.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-TFAM antibody

Reference Product	PubMed Publications
PPARGC1A antibody	>177 publications with TFAM and PPARGC1A
NRF1 antibody	>155 publications with TFAM and NRF1
SIRT1 antibody	>66 publications with TFAM and SIRT1
TFB2M antibody	>48 publications with TFAM and TFB2M
POLRMT antibody	>28 publications with TFAM and POLRMT
TFB1M antibody	>26 publications with TFAM and TFB1M
GABPA antibody	>12 publications with TFAM and GABPA
PPARGC1B antibody	>7 publications with TFAM and PPARGC1B
TP53 antibody	>2 publications with TFAM and TP53
PPRC1 antibody	>2 publications with TFAM and PPRC1

Pathways associated with anti-TFAM antibody

Products by Pathway	Pathway Diagram
Energy Metabolism Pathway antibodies	Energy Metabolism Pathway Diagram
Gene Expression Pathway antibodies	Gene Expression Pathway Diagram
Huntington's Disease Pathway antibodies	Huntington's Disease Pathway Diagram
Huntington's Disease Pathway antibodies	Huntington's Disease Pathway Diagram
Mitochondrial Gene Expression Pathway antibodies	Mitochondrial Gene Expression Pathway Diagram
Mitochondrial Biogenesis Pathway antibodies	Mitochondrial Biogenesis Pathway Diagram
Mitochondrial Transcription Initiation Pathway antibodies	Mitochondrial Transcription Initiation Pathway Diagram
Organelle Biogenesis And Maintenance Pathway antibodies	Organelle Biogenesis And Maintenance Pathway Diagram
RNA Polymerase I, RNA Polymerase III, And Mitochondrial Transcription Pathway antibodies	RNA Polymerase I, RNA Polymerase III, And Mitochondrial Transcription Pathway Diagram
Transcription From Mitochondrial Promoters Pathway antibodies	Transcription From Mitochondrial Promoters Pathway Diagram

Diseases associated with anti-TFAM antibody

Disease Name	Pubmed Publications
Disease Models, Animal Antibodies	>67 publications with TFAM and Disease Models, Animal
Cardiovascular Diseases Antibodies	>62 publications with TFAM and Cardiovascular Diseases
Insulin Resistance Antibodies	>47 publications with TFAM and Insulin Resistance
Inflammation Antibodies	>42 publications with TFAM and Inflammation
Heart Diseases Antibodies	>41 publications with TFAM and Heart Diseases
Necrosis Antibodies	>30 publications with TFAM and Necrosis
Fatty Liver Antibodies	>22 publications with TFAM and Fatty Liver
Liver Diseases Antibodies	>22 publications with TFAM and Liver Diseases
Hyperglycemia Antibodies	>18 publications with TFAM and Hyperglycemia
Alzheimer Disease Antibodies	>18 publications with TFAM and Alzheimer Disease

Organs/Tissues associated with anti-TFAM antibody

Organ/Tissue Name	Pubmed Publications
Muscle Antibodies	>244 publications with TFAM and Muscle
Heart Antibodies	>106 publications with TFAM and Heart
Brain Antibodies	>99 publications with TFAM and Brain
Liver Antibodies	>95 publications with TFAM and Liver
Blood Antibodies	>94 publications with TFAM and Blood
Connective Tissue Antibodies	>35 publications with TFAM and Connective Tissue
Vascular Antibodies	>29 publications with TFAM and Vascular
Lung Antibodies	>24 publications with TFAM and Lung
Kidney Antibodies	>20 publications with TFAM and Kidney
Eye Antibodies	>17 publications with TFAM and Eye