Thr306 Cam Kinase II (CAMK2A/B) Antibody – Rabbit Polyclonal

Polyclonal

Rabbit

Bovine, Canine, Chicken, Human, Xenopus, Zebrafish

100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol.

Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.

Specific for the ~50k alpha-CaM Kinase II and the ~60k beta-CaM Kinase II proteins phosphorylated at Thr306. Immunolabeling is completely eliminated by lambda-phosphatase treatment. It has been reported that this antibody may recognize some level of non-phosphorylated pure recombinant protein, but in our hands in native tissue, the antibody reacts in a phospho-specific manner.

Store at -20 degree C.

~50 kDa; ~60 kDa

ModBase 3D Structure for P11295

Rat

Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr306 conjugated to KLH

Ca2+/Calmodulin-Dependent Protein Kinase II (CaM Kinase II) is a multi-functional calcium and calmodulin-dependent protein kinase that mediates cellular responses to a wide variety of intercellular signals (Kennedy, 1998; Schulman and Hanson, 1993). CaM Kinase II has been shown to regulate diverse cellular functions including synaptic plasticity, neurotransmitter synthesis and release, gene expression, ion channel function, carbohydrate metabolism, cytoskeletal function, and Ca2+-homeostasis (Gleason et al., 2003; Soderling, 2000; Hudmon and Schulman, 2002). Phosphorylation of Thr286 on the kinase produces an autonomously active form of CaM Kinase II (Meng et al., 2003; Picciotto et al., 1993). CaMKIIalpha autophosphorylation at Thr286 and Thr305/Thr306 has recently been shown to regulate kinase activity and modulate subcellular targeting and is critical for normal synaptic plasticity and learning and memory (Baucum et al., 2015).

Phosphopeptide corresponding to amino acid residues surrounding the phospho-Thr306 found in rat brain CaM Kinase II.