Rabbit Anti-AKT1S1 Polyclonal Antibody – Cat# MBS502227

Western blot of Drospohila lysate showing specific labeling of the ~60k PRAS40 protein phosphorylated at Thr356 (Control). Immunolabeling is completely eliminated by treatment with lambda-phosphatase (?-Ptase).

Thr356 PRAS40 (AKT1S1) Antibody – Rabbit Polyclonal

Name: AKT1S1 Antibody; Polyclonal; Rabbit; Drosophila-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS502227
Price: 495 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS502227

MBS502227 is an antibody targeting AKT1S1. Also known as Anti-Phospho-Thr356 PRAS40. Reactive in Drosophila. Host: Rabbit. More details

$495 / 0.1 mL +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Thr356 PRAS40 (AKT1S1), Polyclonal Antibody

Full Product Name

Anti-Phospho-Thr356 PRAS40

Product Gene Name

anti-AKT1S1 antibody

Product Synonym Names

PRAS40 (Thr356)

Clonality

Polyclonal

Host

Rabbit

Species Reactivity

Drosophila

Form/Format

100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol.

Purity/Purification

Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.

Specificity

Specific for the ~60k PRAS40 protein phosphorylated at Thr356. Immunolabeling is completely eliminated with ?-phosphatase treatment.

Preparation and Storage

Store at -20 degree C.

Molecular Weight

~60 kDa

Immunogen Species

Drosophila

Immunogen Info

Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr356 conjugated to KLH

Biological Significance

PRAS40 (proline-rich Akt/PKB substrate of 40 kDa) acts at the intersection of the Akt- and mTOR-mediated signaling pathways and its phosphorylation by Akt and by mTORC1 results in dissociation of PRAS40 from mTORC1 which may relieve an inhibitory constraint on mTORC1 activity (Wiza et al., 2012). Phosphorylation of PRAS40 by Akt and association with 14-3-3 has also been indicated to be crucial for insulin to stimulate mTOR. (Vander Haar et al., 2007). The primary function of PRAS40 in vivo in Drosophila has been shown to regulate TORC1 activity, and not to act as a downstream target and effector of TORC1 (Pallares et al., 2012).

Antigen

Phosphopeptide corresponding to amino acid residues surrounding the phospho-Thr356 of PRAS40.

Applications Tested/Suitable for anti-AKT1S1 antibody

Western Blot (WB)

Application Notes for anti-AKT1S1 antibody

WB: 1:500

Other Notes

Small volumes of anti-AKT1S1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for AKT1S1. It may not necessarily be applicable to this product.

NCBI GI #

NCBI GeneID

NCBI Accession #

GenBank Nucleotide #

NCBI Official Full Name

PRAS40 ortholog

NCBI Official Synonym Full Names

PRAS40 ortholog

NCBI Official Symbol

PRAS40

NCBI Official Synonym Symbols

CG10109; CG46146; DmelCG46146; dPRAS40

Protein Family

Proline-rich AKT1 substrate

NCBI Protein Information

CG46146 gene product from transcript CG46146-RA

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.