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OspC Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS505002

the Borrelia afzelii antigen OspC has been prepared as a recombinant antigen fused to a his-tag in its N-terminus. It is produced from the complete ORF of the gene of the outer surface protein C. More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
OspC, Recombinant Protein
Full Product Name
OspC recombinant
Product Gene Name
Product Synonym Names
RECOMBINANT ANTIGEN ospC from Borrelia afzelii; B. afzelii
Host
E Coli
Form/Format
Dry powder (lyophilized)
Purity/Purification
0.95
Concentration
0.908 mg/ml (lot specific)
Preparation and Storage
Lyophilized protein is shipped with on ice packs. Upon arrival, it should be stored at 20 degree C to -80 degree C. Aliquot after reconstitution in order to avoid repeated freezing and thawing cycles and store at 20 degree C to -80 degree C.
Chromosome Location
NC_008274.1 (16925..17563). Location: plasmid: cp27
Recombinant Antigen
the outer surface protein C (OspC) of Borrelia afzelii (Rousselle et al., 1998).
Organism
Borrelia
Tag
HIS
Molecular Weight Information
Determined by SDS-PAGE, theprotein band is between molecular markers of 25,000 and35,000 Da, while relative molecular mass calculated fromamino acid sequence is 31,320.3 Da.
Observation Notes
in some cases, purified proteins runat a molecular weight which is slightly different to thetheoretically calculated molecular weight maybe due to thehis-tag presence, which can produce a delay in SDS-PAGE.Proteins should be maintained frozen at highconcentrations. The dilution to be performed for ELISAassays should be made with a small quantity of protein, thesame day of the experiment. In order to defrost the protein,maintain the aliquot at 25 degree C without shaking to avoidaggregation. Prior making test dilutions and after defrostthe protein, is recommended to remove possible proteinaggregates by centrifuging the stock solution, avoidingalterations in the immobilization of the biomolecule to thesolid surface.
Buffer
20 mM phosphate buffer pH 8, 1 M NaCl and 0.1% polyoxyethylene (10) tridecyl ether, urea 8 M
Batch Composition
Recombinant antigen with a his-tag in its N-terminus
Storage Buffer
20 mM phosphate buffer pH 8, 1 M NaCl and 0.1% polyoxyethylene (10) tridecyl ether, urea 8 M
Reconstitution
For lyophilized proteins: with 0.04 ml of sterile double-distilled water (lot specific) for a final concentration of 1.59 mg/ml (lot specific). The protein will be at pH 8.

Product Categories/Family for OspC recombinant protein

ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.

Testing Data of OspC recombinant protein

OspC Recombinant Protein – Cat# MBS505002

Testing Data #2 of OspC recombinant protein

OspC Recombinant Protein, Testing Data #2 – Cat# MBS505002

Product References and Citations for OspC recombinant protein

Rousselle J. C., Callister S. M., Schell R. F., Lovrich S.D., Jobe D. A., Marks J. A. and Wieneke C. A.Borreliacidal antibody production against outer surfaceprotein C of Borrelia burgdorferi. 1998, J. Infect. Dis.,178(3):733-41.
Bradford, MM. A rapid and sensitive method for thequantitation of microgram quantities of protein utilizing theprinciple of protein dye binding. Anal Biochem. 1976,131:499-503.

Other Notes

Small volumes of OspC recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for OspC purchase

MBS505002 is a recombinant protein targeting OspC. Also known as OspC recombinant. Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for OspC. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
GenBank Nucleotide #
NCBI Official Full Name
outer surface protein C
NCBI Official Symbol
ospC
Protein Family
NCBI Protein Information
outer surface protein C

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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