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KMP-11 Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS505006

the Lesihmania donovani antigen KMP-11-biotinylated has been prepared as a recombinant antigenfused to a his-tag and biotinylated "in vivo". It is producedfrom the complete ORF of the KMP-11 gene which codifiesthe kinetoplast membrane protein 11. More details

$1195 / 1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
KMP-11, Recombinant Protein
Full Product Name
KMP-11 recombinant
Product Synonym Names
RECOMBINANT ANTIGEN KMP11 from Leishmania donovani; kinetoplastid membrane protein-11
Host
Escherichia coli.
Form/Format
Dry powder (lyophilized)
Concentration
1.59 mg/ml (lot specific)
Preparation and Storage
Lyophilized protein is shipped with on ice packs. Upon arrival, it should be stored at 20 degree C to -80 degree C. Aliquot after reconstitution in order to avoid repeated freezing and thawing cycles and store at 20 degree C to -80 degree C.
Chromosome Location
Chromosome: 35; NC_009419.2 (898529..898807). Location: chromosome: 35
Recombinant Antigen
Lesihmania donovani KMP11 (Passos et al., 2005).
Batch Composition
Components: his-KMP11
Composition: Recombinant antigen with a his-tag in its N-terminus.
Molecular Weight Information
Determined by SDS-PAGE, theprotein band is between molecular markers of 25,000 and18,400 Da, while relative molecular mass calculated fromamino acid sequence is 19,460.0 Da
Observation Notes
Proteins should be maintained frozenat high concentrations. The dilution to be performed for ELISA assays should be made with a small quantity of protein, the same day of the experiment. In order to defrostthe protein, maintain the aliquot at 25°C without shaking toavoid aggregation. Prior making test dilutions and afterdefrost the protein, is recommended to remove possibleprotein aggregates by centrifuging the stock solution,avoiding alterations in the immobilization of the biomoleculeto the solid surface.
Reconstitution
For lyophilized proteins: with 0.04 ml of sterile double-distilled water (lot specific) for a final concentration of 1.59 mg/ml (lot specific). The protein will be at pH 8.

Product Categories/Family for KMP-11 recombinant protein

ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for KMP-11 recombinant protein
ELISA (EIA), Western Blot (WB), Lateral Flow (LF)
Application Notes for KMP-11 recombinant protein
Suggested Titer by ELISA: approx 1/2,800 which corresponds to 0.56 ug/ml of protein concentration in plates for IgG detection.

Product References and Citations for KMP-11 recombinant protein

Passos, S., L. P. Carvalho, G. Orge, S. M. Jeronimo, G.Bezerra, M. Soto, C. Alonso, and E. M. Carvalho.Recombinant Leishmania antigens for serodiagnosis ofvisceral leishmaniasis. 2005, Clinical and Diagnostic Lab.Immunology, 12:1164-1167.
Bradford, MM. A rapid and sensitive method for thequantitation of microgram quantities of protein utilizing theprinciple of protein dye binding. Anal Biochem. 1976,131:499-503.

Other Notes

Small volumes of KMP-11 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for KMP-11 purchase

MBS505006 is a recombinant protein targeting KMP-11. Also known as KMP-11 recombinant. Host: Escherichia coli.

Additional Details

NCBI/Uniprot data below describe general gene information for KMP-11. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
GenBank Nucleotide #
NCBI Official Full Name
kinetoplastid membrane protein-11
NCBI Official Symbol
KMP11-1
NCBI Protein Information
kinetoplastid membrane protein-11

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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