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BALF2 Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS505010

The early antigen p138 has been prepared as a recombinant antigen fused to a his-tag. It is produced from the complete ORF of the BALF2 gene which codifies the major DNA binding protein of EBV. More details

$1195 / 1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
BALF2, Recombinant Protein
Full Product Name
BALF2 recombinant
Product Gene Name
BALF2 recombinant protein
Product Synonym Names
RECOMBINANT ANTIGEN p138 from Epstein-Barr Virus (EBV); p138 (D-EA)
Host
E Coli
Form/Format
Dry powder (lyophilized)
Purity/Purification
0.95
Concentration
1.59 mg/ml (lot specific)
Preparation and Storage
Lyophilized protein is shipped with on ice packs. Upon arrival, it should be stored at 20 degree C to -80 degree C. Aliquot after reconstitution in order to avoid repeated freezing and thawing cycles and store at 20 degree C to -80 degree C.
Molecular Weight
123,122 Da
Chromosome Location
NC_007605.1 (160550..164356, complement)
3D Structure
ModBase 3D Structure for P03227
Recombinant Antigen
EBV antigen p138 (Mumtsidu et al., 2008).
Organism
EBV
Tag
HIS
Molecular Weight Information
Determined by SDS-PAGE, the protein band is between molecular markers of 66,200-116,000 Da, while relative molecular mass calculated from amino acid sequence is 129,700.0 Da.
Suggested Titer by ELISA
approx. 1:3250, which corresponds to 0.49ug/ml of protein concentration in plates for IgG detection.
Observation Notes
Proteins should be maintained frozen at high concentrations. The dilution to be performed for ELISA assays should be made with a small quantity of protein, the same day of the experiment. In order to defrost the protein, maintain the aliquot at 25 degree C without shaking to avoid aggregation. Prior making test dilutions and after defrost the protein, is recommended to remove possible protein aggregates by centrifuging the stock solution, avoiding alterations in the immobilization of the biomolecule to the solid surface.
Buffer
20 mM phosphate buffer pH 8, 1 M NaCl, 0.1% polyoxyethylene (10) tridecyl ether and 6 M urea
Batch Composition
Recombinant antigen with a his-tag in its N-terminus
Storage Buffer
20 mM phosphate buffer pH 8, 1 M NaCl, 0.1% polyoxyethylene (10) tridecyl ether and 6 M urea
Reconstitution
For lyophilized proteins: with 0.04 ml of sterile double-distilled water (lot specific) for a final concentration of 1.59 mg/ml (lot specific). The protein will be at pH 8.

Product Categories/Family for BALF2 recombinant protein

Viruses
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for BALF2 recombinant protein
ELISA (EIA), Western Blot (WB)
Application Notes for BALF2 recombinant protein
Recommended concentration in ELISA plates: 2 ug/ml in plates for IgM detection

Testing Data of BALF2 recombinant protein

BALF2 Recombinant Protein – Cat# MBS505010

Testing Data #2 of BALF2 recombinant protein

BALF2 Recombinant Protein, Testing Data #2 – Cat# MBS505010

Product References and Citations for BALF2 recombinant protein

Mumtsidu E, Makhov AM, Konarev PV, Svergun DI, Griffith JD, Tucker PA. Structural features of the single-stranded DNA-binding protein of Epstein-Barr virus. 2008 J. Struct. Biol. 161(2):172-87.
Gill SC, von Hippel PH. Calculation of protein extinction coefficients from amino acid sequence data. Anal Biochem. 1989 Nov 1;182(2):319-26.

Other Notes

Small volumes of BALF2 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for BALF2 purchase

MBS505010 is a recombinant protein targeting BALF2. Also known as BALF2 recombinant. Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for BALF2. It may not necessarily be applicable to this product.

NCBI GI #
82503273
NCBI GeneID
3783678
NCBI Accession #
YP_401717.1
UniProt Primary Accession #
P03227
UniProt Secondary Accession #
Q777A7
UniProt Related Accession #
Q777A7
GenBank Nucleotide #
NC_007605.1
NCBI Official Full Name
BALF2
NCBI Official Symbol
BALF2
UniProt Protein Name
Major DNA-binding protein
UniProt Entry Name
DNBI_EBVB9
NCBI Protein Information
single-stranded DNA binding protein

UniProt Comments for BALF2

Function: Plays a role in virus genomic DNA replication associated with the viral DNA polymerase complex. Destabilizes double-stranded DNA in an ATP independent manner. May disrupt loops, hairpins and other secondary structures present on ssDNA by binding directly to it, thereby reducing and eliminating pausing of the viral DNA polymerase at specific sites. May participate in the opening of the lytic phase origin (ori-Lyt) region of the viral DNA to initiate viral replication. Ref.2

Subunit structure: Forms a complex with the DNA polymerase BALF5, the DNA polymerase processivity factor BMRF1, and the alkaline exonuclease BGLF5.

Subcellular location: Virion tegument. Host nucleus. Note: the protein is present at discrete sites in nuclei, called replication compartments where viral DNA replication occurs. Ref.4 Ref.5

Sequence similarities: Belongs to the herpesviridae DNA-binding protein family.
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Research Articles on BALF2

1. BMRF1 protein is a multifunctional protein that cannot only act as a DNA polymerase processivity factor but also enhances BALF2 promoter transcription as a coactivator for the BZLF1 protein

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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