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BKRF1 Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS505015

the latent nuclear antigen EBNA-1 has been prepared as a recombinant antigen fused to a his-tag in its N-terminal. It is produced from the complete ORF of the BKRF1 gene of EBV optimized regarding the codon usage of Escherichia coli. More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
BKRF1, Recombinant Protein
Full Product Name
BKRF1 recombinant
Product Gene Name
BKRF1 recombinant protein
Product Synonym Names
RECOMBINANT ANTIGEN EBNA1 from Epstein-Barr Virus (EBV); EBNA-1
Host
E Coli
Form/Format
Dry powder (lyophilized)
Purity/Purification
0.95
Concentration
0.65 mg/ml (lot specific)
Preparation and Storage
Lyophilized protein is shipped with on ice packs. Upon arrival, it should be stored at 20 degree C to -80 degree C. Aliquot after reconstitution in order to avoid repeated freezing and thawing cycles and store at 20 degree C to -80 degree C.
Molecular Weight
56,427 Da
3D Structure
ModBase 3D Structure for P03211
Recombinant Antigen
EBV antigen EBNA1 (Cheng et al., 1991).
Organism
EBV
Tag
HIS
Molecular Weight Information
Determined by SDS-PAGE, the protein band is between molecular markers of 66,200-116,000 Da, while relative molecular mass calculated from amino acid sequence is 63,060.5 Da.
Observation Notes
in some cases, purified proteins run at a molecular weight which is slightly different to the theoretically calculated molecular weight maybe due to the his-tag presence, which can produce a delay in SDS-PAGE. Proteins should be maintained frozen at high concentrations. The dilution to be performed for ELISA assays should be made with a small quantity of protein, the same day of the experiment. In order to defrost the protein, maintain the aliquot at 25 degree C without shaking to avoid aggregation. Prior making test dilutions and after defrost the protein, is recommended to remove possible protein aggregates by centrifuging the stock solution, avoiding alterations in the immobilization of the biomolecule to the solid surface.
Buffer
20 mM phosphate buffer pH 8, 1 M NaCl, 0.1% polyoxyethylene (10) tridecyl ether and 6 M urea
Batch Composition
Recombinant antigen with a his-tag in its N-terminus
Storage Buffer
20 mM phosphate buffer pH 8, 1 M NaCl, 0.1% polyoxyethylene (10) tridecyl ether and 6 M urea
Reconstitution
For lyophilized proteins: with 0.04 ml of sterile double-distilled water (lot specific) for a final concentration of 1.59 mg/ml (lot specific). The protein will be at pH 8.

Product Categories/Family for BKRF1 recombinant protein

Viruses
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for BKRF1 recombinant protein
ELISA (EIA), Western Blot (WB)
Application Notes for BKRF1 recombinant protein
Recommended concentration in ELISA plates: 0.11 ug/ml in plates for IgG detection
Suggested Titer by ELISA: 1:6000, which corresponds to 0.11 g/ml in plates for IgG detection

Testing Data of BKRF1 recombinant protein

BKRF1 Recombinant Protein – Cat# MBS505015

Testing Data #2 of BKRF1 recombinant protein

BKRF1 Recombinant Protein, Testing Data #2 – Cat# MBS505015

Product References and Citations for BKRF1 recombinant protein

Cheng, H., Y. Foong, C. Sam, U. Prasad and J. Dillner. Epstein-Barr Virus Nuclear Antigen 1 Linear Epitopes That Are Reactive with Immunoglobulin A (IgA) or IgG in Sera from Nasopharyngeal Carcinoma Patients or from Healthy Donors. 1991. J. Clin. Microbiol. 29:2180-218.
Gill SC, von Hippel PH. Calculation of protein extinction coefficients from amino acid sequence data. Anal Biochem. 1989 Nov 1;182(2):319-26.

Other Notes

Small volumes of BKRF1 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for BKRF1 purchase

MBS505015 is a recombinant protein targeting BKRF1. Also known as BKRF1 recombinant. Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for BKRF1. It may not necessarily be applicable to this product.

NCBI GI #
23893623
NCBI Accession #
CAD53427.1
UniProt Primary Accession #
P03211
UniProt Secondary Accession #
Q777E1
NCBI Official Full Name
EBNA-1 protein
UniProt Protein Name
Epstein-Barr nuclear antigen 1
UniProt Gene Name
EBNA1
UniProt Synonym Gene Names
EBNA-1
UniProt Entry Name
EBNA1_EBVB9

UniProt Comments for BKRF1

Function: Plays an essential role in replication and partitioning of viral genomic DNA during latent viral infection. During this phase, the circular double-stranded viral DNA undergoes replication once per cell cycle and is efficiently partitioned to the daughter cells. EBNA1 activates the initiation of viral DNA replication through binding to specific sites in the viral latent origin of replication, oriP. Additionally, it governs the segregation of viral episomes by mediating their attachment to host cell metaphase chromosomes. Also activates the transcription of several viral latency genes. Finally, it can counteract the stabilization of host p53/TP53 by host USP7, thereby decreasing apoptosis and increasing host cell survival. Ref.8

Subunit structure: Interacts with human USP7. Interacts with human EBP2; this interaction is important for the stable segregation of EBV episomes during cell division but not for the replication of the episomes. Interacts with BGLF4; this interaction facilitates the switch from latent to lytic DNA replication by down-regulating EBNA1 replication function. Ref.4 Ref.5 Ref.6 Ref.8

Subcellular location: Host nucleus Ref.3.

Sequence similarities: Belongs to the herpesviridae EBNA1 family.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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