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BZLF1 Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS505029

Recombinant Antigen: Epstein-Barr virus zebra (Yamauchiet al., 1998)The EBV gene BZLF1 has been expressed as a recombinant antigen zebra. It is produced from the complete ORF of the bZIP transcription factor. More details

$1195 / 1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
BZLF1, Recombinant Protein
Full Product Name
BZLF1 recombinant
Product Gene Name
BZLF1 recombinant protein
Product Synonym Names
Zebra (EA)
Host
E. coli
Form/Format
Dry powder (lyophilized)
Preparation and Storage
Protein is shipped on blue ice. Upon arrival, it should be stored at 4°C or -20°C. Once reconstituted, it should be aliquoted in order to avoid repeated freezing and thawing cycles and stored at -20°C to -80°C.
Molecular Weight Note
Determined by SDS-PAGE, the protein band is at the molecular marker between molecular markers of 45,000 Da and 35,000 Da, while relative molecular mass calculated from amino acid sequence is 35,081.1 Da.
Batch Composition
Components: his-zebra
Composition: recombinant antigen with a his-tag in its N-terminus

Components: Storage buffer befor lyophilization
Composition: 20 mM phosphate buffer pH8, 1M NaCl and 4 M urea.
Quality Control
Protein Concentration Determined Espectrophotometrically:
DO280 = 0.722
A0.1% (=1 g/l) = 0.653
Concentration*: 1.29 mg/ml
*The measurement of the protein concentration has been performed with the theoretical extinction coefficient of the recombinant protein obtained from Gill and vonHippel, 1989.
Reconstitution
With approx. 0.79 ml of sterile double-distilled water for a final concentration of approx. 1.10 mg/ml. The protein will be at pH 8.
Total Quantity per Aliquot
1 mg
Observation
proteins should be maintained frozen at high concentrations. The dilution to he performed for ELISA assays should be made with a small quantity of protein, the same day of the experiment. In order to defrost the protein, maintain the aliquot at 25 degree C without shaking to avoid aggregation. Prior making test dilutions and after defrosting the protein, is recommended to remove possible protein aggregates by centrifuging the stock solution, avoiding alterations in the immobilization of the biomolecule to the solid surface.

Product Categories/Family for BZLF1 recombinant protein

Viruses
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for BZLF1 recombinant protein
ELISA, Western Blot (WB)
Application Notes for BZLF1 recombinant protein
Suggested Titer by ELISA: Up to 1:1,183, which corresponds to 0.93 ug/ml of protein concentration in plates for IgG detection.

Where this product has not been tested for use in a particular technique, this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates.

Proteins should be maintained frozen at high concentrations. The dilution to be performed for ELISA assays should be made with a small quantity of protein, the same day of the experiment. In order to defrost the protein, maintain the aliquot at 25°C without shaking to avoid aggregation. Prior making test dilutions and after defrost the protein,is recomended to remove possible protein aggregates by centrifuging the stock solution, voiding alterations in the immobilization of the biomolecule to the solid surface.

ELISA of BZLF1 recombinant protein

Titration Curve by an ELISA Assay. The titer has been suggested in reference to an "in-house" ELISA kit performed at the lab over the first lot obtained. Each end user should carry out their own titration for their particular application. In this plot, the optical density at 450/620 nm for positive (blue) and negative (gray) IgG sera are compared for each concentration of the recombinant antigen. An appropriate statistical test of significance for the comparison of means between both groups, the Welch's test, is employed. Eligible concentrations for the use of the antigen should present statistically significant differences between positive and negative sera. This happens when the intervals at the top do not overlap and, equivalently, when the value at the bottom is below 0.05. In the present figure, all p-values are below 0.05 and thus the intervals do not overlap. Therefore, any of the showed concentrations can be used to distinguish between positive and negative sera.
BZLF1 Recombinant Protein – Cat# MBS505029

SDS-PAGE of BZLF1 recombinant protein

SDS-PAGE analysis (15%) of 2 uL of recombinant ZEBRA. Purity is >98% as determined by gel electrophoresis.
BZLF1 Recombinant Protein, SDS-PAGE – Cat# MBS505029

Other Notes

Small volumes of BZLF1 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for BZLF1 purchase

MBS505029 is a recombinant protein targeting BZLF1. Also known as BZLF1 recombinant. Host: E. coli.

Additional Details

NCBI/Uniprot data below describe general gene information for BZLF1. It may not necessarily be applicable to this product.

NCBI GI #
808657
NCBI Accession #
AAA66529.1
NCBI Official Full Name
BZLF1
Protein Family
Trans-activator protein

UniProt Comments for BZLF1

Plays a key role in the switch from latent infection to lytic cycle producing new virions. Acts as a transcription factor, inducing early lytic cycle genes, and as a origin binding protein for genome replication. BZLF1 activates the promoter of another EBV gene (BSLF2+BMLF1).

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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