lipl32 Recombinant Protein

Escherichia Coli.

20 mM phosphate buffer pH 6 and 0.15 M NaCl and 0.1 polyoxythylene (10) tridecyl ether.(before lyophilization)

1.16 mg/ml (lot specific)

Protein is shipped at room temperature. Upon receipt, it should be stored at 4° to -20°C in vertical position, avoiding all possible humidity and maintaining the vials dry. Once reconstituted, it should be aliquoted to avoid repeated freezing and thawing cycles and stored at -20ºC to -80ºC.

Determined by SDS-PAGE, the protein band is at the molecular marker of 35,000 Da, while relative molecular mass calculated from amino acid sequence is 34,961.4 Da.

Escherichia coli

dry powder (stabilized with 15% trehalose)

Protein Concentration Determined Espectrophotometrically:
DO₂₈₀ = 1.08
A_{0.1 %} (=1 g/l) = 0.923

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Proteins should be maintained frozen at high concentrations. The dilution to be performed for ELISA assays should be made with a small quantity of protein, the same day of the experiment. In order to defrost the protein, maintain the aliquot at 25ºC without shaking to avoid aggregation. Prior making test dilutions and after defrosting the protein, is recommended to remove possible protein aggregates by centrifuging the stock solution, avoiding alterations in the immobilization of the biomolecule to the solid surface.

With approx. 0.802 ml of sterile double-distilled water, a final concentration of approx. 1.16 mg/ml will be obtained. The solubilisation of the cake should be developed for 15 min to allow a homogeneous protein solution, considering that part of the cake can be on the glass-walls of the container. Please keep in mind that the dinal volume of the protein solution may differ from the reconstitution volume mentioned in the instructions due to the hygroscopic nature of trehalose. As the protein is reconstituted, the final volume may slightly increase to reach the specified amount mentioned in te certificate of analysis. Upon reconstitution, leave the solution at least 15 min. homogenizing with a mild agitation at 4°C. Avoid vigorous shaking that can cause foaming and protein denaturation . After those minutes , centrifuge the vial to ensure that all the product remains at the base and do not lose any of it on the walls. With this reconsitution , the protein will be maintained at pH6. It is recommended that the users carry out their absorbance determinations to avoid concentration variabilities due to the equiptment used,mainly in reproducibility analysis.

1 mg

Manufactured in an ISO 9001:2015 Certified Laboratory.