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P54 (BMRF1) Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS505054

The diffuse early antigen p54 has been prepared as a recombinant antigen fused to a his-tag at the N-terminal. It is produced from the C-terminal fragment of the EBV BMRF1 gene. More details

$1195 / 1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
p54 (BMRF1), Recombinant Protein
Full Product Name
RECOMBINANT ANTIGEN p54 from Epstein-Barr Virus (EBV)
Product Gene Name
Host
E Coli
Form/Format
Dry powder (lyophilized)
Concentration
0.467 mg/ml (lot specific)
Preparation and Storage
Lyophilized protein is shipped with on ice packs. Upon arrival, it should be stored at 20 degree C to -80 degree C. Aliquot after reconstitution in order to avoid repeated freezing and thawing cycles and store at 20 degree C to -80 degree C.
Molecular Weight
Determined by SDS-PAGE, the protein band is between molecular markers of 35,000-25,000 Da, while relative molecular mass calculated from amino acid sequence is 20,342.3 Da.
Observations
Proteins should be maintained frozen at high concentrations. The dilution to be performed for ELISA assays should be made with a small quantity of protein, the same day of the experiment. In order to defrost the protein, maintain the aliquot at 25 degree C without shaking to avoid aggregation. Prior making test dilutions and after defrost the protein, is recommended to remove possible protein aggregates by centrifuging the stock solution, avoiding alterations in the immobilization of the biomolecule to the solid surface.
Organism
EBV
Antigen
P54 (D-EA)
Recombinant Antigen
EBV antigen p54 (Neuhierl et al., 2006).
Componets
Recombinant antigen with a a his-tag in its N-terminus
Storage Buffer
20 mM phosphate buffer pH 8, 1 M NaCl, 0.1% polyoxyethylene (10) tridecyl ether and 6 M urea
Reconstitution
For lyophilized proteins: with 0.04 ml of sterile double-distilled water (lot specific) for a final concentration of 1.59 mg/ml (lot specific). The protein will be at pH 8.

Product Categories/Family for BMRF1 recombinant protein

ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for BMRF1 recombinant protein
ELISA (EIA), Western Blot (WB)
Application Notes for BMRF1 recombinant protein
1:300, which corresponds to 1.16 ug/ml of protein concentration in plates for IgG detection and 1:200, which corresponds to 2.32 ug/ml of protein concentration in plates for IgM detection.
Where this product has not been tested for use in a particular technique, this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates.

Testing Data #2 of BMRF1 recombinant protein

BMRF1 Recombinant Protein, Testing Data #2 – Cat# MBS505054

Product References and Citations for BMRF1 recombinant protein

Neuhierl, B. and Henri-Jacques Delecluse, H.J. The Epstein-Barr Virus BMRF1 Gene Is Essential for Lytic Virus Replication. Journal of Virology, 2006, Vol. 80, No. 10: 5078-5081.
Gill SC, von Hippel PH. Calculation of protein extinction coefficients from amino acid sequence data. Anal Biochem. 1989 Nov 1;182(2):319-26.

Other Notes

Small volumes of BMRF1 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for BMRF1 purchase

MBS505054 is a recombinant protein targeting BMRF1. Also known as RECOMBINANT ANTIGEN p54 from Epstein-Barr Virus (EBV). Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for BMRF1. It may not necessarily be applicable to this product.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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