CFP10 Recombinant Protein

Name: CFP10 Recombinant Protein; Research Grade; MyBioSource
Brand: MyBioSource
SKU: MBS505077
Price: 1195 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS505077

The Mycobacterium tuberculosis gene for the 10 kDa culture filtrate antigen EsxB, has been expressed as a recombinant antigen fused to a his-tag in its N-terminus. More details

$1195 / 1 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

CFP10, Recombinant Protein

Full Product Name

Recombinant antigen CFP10 for Mycobacterium tuberculosis (Koch's bacillus)

Product Gene Name

CFP10 recombinant protein

Product Synonym Names

esxB

Host

Escherichia Coli.

Form/Format

Dry powder (stabilized with 5% trehalose)

Preparation and Storage

Upon arrival, it should be stored at 4°C to -20°C in vertical position, avoiding all possible humidity and maintaining the vials dry. Once reconstituted, it should be aliquoted to avoid repeated freezing and thawing cycles and stored at -20°C to -80°C.

Molecular Weight

Determined by SDS-PAGE, the protein band is between molecular markers of 25,000 and 18,400 Da, while relative molecular mass calculated from amino acid sequence is 17,506.0 Da.

Composition

Components: his-CFP10
Composition: recombinant antigen with a his-tag in its N-terminus.

Components: Storage buffer before lyophilization
Composition: 20 mM phosphate buffer pH 6.5, 0.15 M NaCl and 0.1% polyoxyethylene (10) tridecyl ether

Quality Control

Protein Concentration Determined Espectrophotometrcially:
DO₂₈₀ = 0.66
A_0.1% (=1 g/l) = 0.484
Concentration: 1.37 mg/ml

Absence of precipitation after a freezing and thawing cycle

Observations

Proteins should be maintained frozen at high concentrations. The dilution to be performed for ELISA assays should be made with a small quantity of protein, the same day of the experiment. In order to defrost the protein, maintain the aliquot at 25ºC without shaking to avoid aggregation. Prior making test dilutions and after defrosting the protein, is recommended to remove possible protein aggregates by centrifuging the stock solution, avoiding alterations in the immobilization of the biomolecule to the solid surface.

Total Quantity Per Aliquot

1mg

Reconstitution

with approx. 0.670 ml of sterile double-distilled water, a final concentration of approx. 1.37 mg/ml will be obtained. The solubilization of the cake should be developed for 15 min to allow a homogeneous protein solution, considering that part of the cake can be on the glass-walls of the container. Upon reconstitution, leave the solution at least 15 min homogenizing with a mild agitation at 4°C. Avoid vigorous shaking that can cause foaming and protein denaturation. With this reconstitution, the protein will be maintained at pH 6.5. It is recommended that the users carry out their absorbance determinations to avoid concentration variabilities due to the equipment used, mainly in reproducibility analysis.

Product Categories/Family for CFP10 recombinant protein

BACTERIA

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Application Notes for CFP10 recombinant protein

Possible Applications : WB, DB, Indirect ELISA , positive control in direct ELISA , CLIA , lateral flow. Where this product has not been tested for use in a particular technique, this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user tirates.

*The mesurement of the protein concentration has been performed with the theoretical extinction coefficient of the recombinant protein obtained from Gill and vonHippel, 1989. It is recommended that the users carry out their absorbance determinations to avoid equipment variabilities regarding final concentration, mainly in reproducibility analysis.

Other Notes

Small volumes of CFP10 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CFP10 purchase

MBS505077 is a recombinant protein targeting CFP10. Also known as Recombinant antigen CFP10 for Mycobacterium tuberculosis (Koch's bacillus). Host: Escherichia Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for CFP10. It may not necessarily be applicable to this product.

NCBI GI #

597960710

NCBI Accession #

AHN50412.1

NCBI Official Full Name

CFP10, partial

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.