Figure 1. SDS-PAGE analysis (15%) of 10 ul of the recombinant allergen Der f 2. Purity is >95% as determined by gel electrophoresis.

DER f 2 Recombinant Protein

Name: DER f 2 Recombinant Protein; Research Grade; MyBioSource
Brand: MyBioSource
SKU: MBS505094
Price: 1335 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS505094

Recombinant Allergen: Dermatophagoides farinae Der f 2 (Heyman et al., 1989).Description: Der f 2 is one of the major allergens of the American house dust mite. This allergen is of the NPC2 family and it has been produced as a recombinant mature protein fused… More details

$1335 / 1 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Der f 2, Recombinant Protein

Full Product Name

Recombinant allergen Der f 2 for Dermatophagoides farinae (American house dust mite)

Product Gene Name

Der f 2 recombinant protein

Host

Pichia pastoris

Form/Format

Dry powder (lyophilized)

Purity/Purification

15% SDS-PAGE

Concentration

1.088 mg/ml (lot specific)

Preparation and Storage

Lyophilized protein is shipped with on ice packs. Upon arrival, it should be stored at 20 degree C to -80 degree C. Aliquot after reconstitution in order to avoid repeated freezing and thawing cycles and store at 20 degree C to -80 degree C.

Molecular Weight

Predicted MW: 16.5 kDa
Observed MW: 18.4 kDa

Organism

Acarus

Storage Buffer

50 mM MES pH 6 and 0.1 M KCl

Quality Control

1. Protein concentration determined spectrophotometrically.
2. Purity control in SDS-PAGE: 15%
3. No precipitate observed after a freezing and thawing cycle.

Reconstitution

For lyophilized proteins: with 0.04 ml of sterile double-distilled water (lot specific) for a final concentration of 1.59 mg/ml (lot specific). The protein will be at pH 8.

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Product References and Citations for Der f 2 recombinant protein

Heyman PW, Chapman MD, Fox J, Aalberse, RC, PlattsMills, TAE. Antigenic and structural analysis of the Group II allergens (Derp II and Der f II) from house dust mites (Dermatophagoides sp). J Allergy Clin Immunology 1989; 83:1055-67.
Gill SC, von Hippel PH. Calculation of protein extinction coefficients from amino acid sequence data. Anal Biochem. 1989 Nov 1;182(2):319-26.

Other Notes

Small volumes of Der f 2 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Der f 2 purchase

MBS505094 is a recombinant protein targeting Der f 2. Also known as Recombinant allergen Der f 2 for Dermatophagoides farinae (American house dust mite). Host: Pichia pastoris.

Additional Details

NCBI/Uniprot data below describe general gene information for Der f 2. It may not necessarily be applicable to this product.

Protein Family

Mite group 2 allergen

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.