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CAN f 5 Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS505100

Recombinant Allergen: Canis familiaris Can f 5 (Mattsson et al., 2009).Description: The Canis familiaris prostatic kallikrein, Can f 5, has been prepared as a recombinant mature allergen fused to a his-tag. More details

$1335 / 1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Can f 5, Recombinant Protein
Full Product Name
Recombinant allergen Can f 5 for Canis familiaris (Dog)
Product Gene Name
Can f 5 recombinant protein
Host
Pichia pastoris
Form/Format
Dry powder (lyophilized)
Purity/Purification
12% SDS-PAGE
Concentration
1 mg/ml (lot specific)
Preparation and Storage
Lyophilized protein is shipped with on ice packs. Upon arrival, it should be stored at 20 degree C to -80 degree C. Aliquot after reconstitution in order to avoid repeated freezing and thawing cycles and store at 20 degree C to -80 degree C.
Molecular Weight
Predicted MW: 30.4 kDa
Observed MW: 25-35 kDa
Organism
Epithelia
Storage Buffer
20 mM phosphate buffer pH 8, 5 mM EDTA and 0.15 M NaCl
Quality Control
1. Protein concentration determined spectrophotometrically.
2. Purity control in SDS-PAGE: 12%
3. No precipitate observed after a freezing and thawing cycle.
Protein Fingerprint by Mass Spectrometry
Top Score: 76 for Can f 5
Protein score is -10*Log(P), where P is the probability that the observed match is a random event. Protein scores greater than 16 are significant (p<0.05).
The MS was performed with a by MALDI TOF/TOF model UltrafleXtreme (Bruker).
Reconstitution
For lyophilized proteins: with 0.04 ml of sterile double-distilled water (lot specific) for a final concentration of 1.59 mg/ml (lot specific). The protein will be at pH 8.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.

SDS-Page of Can f 5 recombinant protein

Figure 1. SDS-PAGE analysis (12%) of 10 ul of recombinant allergen. Purity is >95% as determined by gel electrophoresis. Bands slightly smaller correspond to the same protein.
CAN f 5 Recombinant Protein – Cat# MBS505100

Testing Data of Can f 5 recombinant protein

CAN f 5 Recombinant Protein, Testing Data – Cat# MBS505100

Product References and Citations for Can f 5 recombinant protein

Mattsson et al. prostatic kallikrein: a new major dog allergen. 2009, J Allergy Clin Immunol 2009; 123:362-368
Gill SC, von Hippel PH. Calculation of protein extinction coefficients from amino acid sequence data. Anal Biochem. 1989 Nov 1;182(2):319-26.

Other Notes

Small volumes of Can f 5 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Can f 5 purchase

MBS505100 is a recombinant protein targeting Can f 5. Also known as Recombinant allergen Can f 5 for Canis familiaris (Dog). Host: Pichia pastoris.
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