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FEL d 1 Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS505101

The Felis domesticus uteroglobin Fel d 1 has been prepared as the recombinant mature protein composed of two chains and fused to a his-tag. Fel d 1 chain 1 and chain 2 are fused by a peptide linker y N-glycosylation motif at position 103 was mutated. Tetramer… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Fel d 1, Recombinant Protein
Full Product Name
Recombinant allergen Fel d 1 for Felis domesticus (cat)
Product Gene Name
Fel d 1 recombinant protein
Host
Pichia pastoris
Form/Format
Dry powder (stabilized with 5 % trhalose) ; 20 mM HEPES buffer pH 8 and 0.15 M NaCl (Before lyophilization)
Concentration
1.92 mg/ml (lot specific)
Preparation and Storage
Upon receipt , store at 4°C to -20°C in vertical position, avoiding all possible humidity and maintaining the viasl dry. Once reconstituted, it shold be aliquoted to avoid freezing and thawing cycles and stored at -20°C to -80°C.
Molecular Weight
SDS-PAGE analysis determines that the protein band is between molecular markers of 35,000 Da and 25,000 Da due to the glycosylation pattern, while relative molecular mass, calculated from amino acid sequence and without glycosylation, is 22,090.19 Da.
Batch Composition
- Components: his-Fel d 1
- Composition: recombinant allergen with a his-tag
Absence of Precipitation After a Freezing and Thawing Cycle
OK
Recombinant Allergen
Fel d 1 is the major allergen of Felis domesticus (cat) (Ohman et al., 1976).
Observations
Proteins should be maintained frozen at high concentrations. In order to defrost the protein, maintain the aliquot at 25°C without shaking to avoid aggregation. Prior to making test dilutions and after defrosting the protein, it is recommended to remove possible protein aggregates by centrifuging the stock solution, avoiding alterations in the immobilization of the biomolecule to the solid surface.
Reconstitution
With 0.461 ml of sterile double-distilled water for a final concentration of 1.92 mg/mL will be obtained. The solubilization of the cake should be developed for 15 minutes to allow a homogenous protein solution, considering that part of the cake can be on the glass-walls of the container. Upon reconstitution, leave the solution at least 15min. homogenizing with a mild agitation at 4°C. Avoid vigorous shaking that can cause foaming ans protein denaturation. With this reconstitution, the protein will be maintained at 8. It is recommended that the users carry out their absorbance determinaters to avoid concentrations variabilites due to the equipment used, mainly in reproducibility analysis.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for Fel d 1 recombinant protein
ELISA (EIA)
Application Notes for Fel d 1 recombinant protein
Possible Applications: Western Blot (WB), Dot Blot (DB), Indirect ELISA, Positive Control in Direct ELISA, Chemiluminescence Immunoassays (CLIA), Lateral Flow (LF). Where this product has not been tested for use in a particular technique, this does not necessarily include its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates. The lab strongly recommends utilizing anti-CCD (cross-reactive-carbohydrate determinants) absorbent to eliminate the anti-CCD IgE antibodies present in normal human sera to prevent cross-reaction of this glycoprotein. By using this absorbent, reactions resulting from CCDs are stopped. In the specific case, you measure IgG with an allergen produced in P. pastoris, the lab recommends using an anti-CCD sorbent (Please Inquire) to avoid cross-reaction with antibodies in sera which react with fungal high mannose glycans.

ELISA (EIA) of Fel d 1 recombinant protein

Protein concentration determined espectrophotometrically
This protein does not contain any Trp residues. Experience shows that this could result in more than 10% error in the computed extinction coefficient. Therefore, we have measured the protein concentration by using the colorimetric assay based on the interaction between Coomassie brilliant blue and the arginine and aromatic residues (Bradford Method) and its maximum absorption shifts from 470 nm to 595 nm. The standard curve was performed with the protein BSA.
FEL d 1 Recombinant Protein – Cat# MBS505101

SDS-PAGE of Fel d 1 recombinant protein

Purity Control in SDS-PAGE: 15%
SDS-PAGE analysis (15%) of 10 ul of recombinant allergen. Purity is >95% as determined by gel electrophoresis.
FEL d 1 Recombinant Protein, SDS-PAGE – Cat# MBS505101

Product References and Citations for Fel d 1 recombinant protein

Ohman, J. L., Lowell, F. C., Bloch, K. J. and Kendall, S. Allergens of mammalian origin V. properties of extracts derived from domestic cat. 1976, Clin. Allergy 6:419-28.
Calculation of protein extinction coefficients from amino acid sequence data. Anal Biochem. 1989 Nov 1;182(2):319-26.

Other Notes

Small volumes of Fel d 1 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Fel d 1 purchase

MBS505101 is a recombinant protein targeting Fel d 1. Also known as Recombinant allergen Fel d 1 for Felis domesticus (cat). Host: Pichia pastoris.

Additional Details

NCBI/Uniprot data below describe general gene information for Fel d 1. It may not necessarily be applicable to this product.

Protein Family
Major allergen I polypeptide

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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