Chicken AMYALPHA ELISA Kit – Cat# MBS051145

Amylase Alpha (AMYALPHA) ELISA Kit for Chicken

Name: Chicken AMYALPHA ELISA Kit; Amylase Alpha; MyBioSource
Brand: MyBioSource
SKU: MBS051145
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS051145

Background/Introduction: This Quantitative Sandwich ELISA kit is for lab reagent/research use only, not for drug, household, therapeutic or test applications! This kit is intended to be used to determine the level of AMYA (hereafter termed "analyte") in… More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

Amylase Alpha (Amyalpha), ELISA Kit

Full Product Name

Chicken Amylase Alpha ELISA Kit

Product Gene Name

Amyalpha elisa kit

Species Reactivity

Chicken

Assay Type

Quantitative Sandwich

Detection Range

0.625ng/ml-20ng/ml

Sensitivity

0.1ng/ml

Preparation and Storage

Store all reagents at 2-8 degree C

Inter-assay Precision

Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]. All CV% should be compared by concentration, not compared by OD values.

Intra-assay Precision

Intra-assay CV (%) is less than 15%.

Molecular Weight

57,707 Da

OMIM

104650

3D Structure

ModBase 3D Structure for P04746

Other Notes

Small volumes of Amyalpha elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Amyalpha purchase

MBS051145 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Amylase Alpha (Amyalpha) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Amyalpha. The ELISA analytical biochemical technique of the MBS051145 kit is based on Amyalpha antibody-Amyalpha antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Amyalpha antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, Amyalpha. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for Amyalpha. It may not necessarily be applicable to this product.

NCBI GI #

529397

NCBI GeneID

279

NCBI Accession #

AAA51724.1

UniProt Primary Accession #

P04746

UniProt Secondary Accession #

Q9UBH3

UniProt Related Accession #

P04746

NCBI Official Full Name

amylase

NCBI Official Synonym Full Names

amylase, alpha 2A (pancreatic)

NCBI Official Symbol

AMY2A

NCBI Official Synonym Symbols

PA; AMY2; AMY2B

UniProt Protein Name

Pancreatic alpha-amylase

UniProt Synonym Protein Names

1,4-alpha-D-glucan glucanohydrolase

UniProt Gene Name

AMY2A

UniProt Synonym Gene Names

UniProt Entry Name

AMYP_HUMAN

NCBI Protein Information

pancreatic alpha-amylase; glycogenase; found in the pancreas; pancreatic amylase 2A; pancreatic amylase alpha 2A; amylase, pancreatic, alpha-2A; 1,4-alpha-D-glucan glucanohydrolase

NCBI Summary for Amyalpha

Amylases are secreted proteins that hydrolyze 1,4-alpha-glucoside bonds in oligosaccharides and polysaccharides, and thus catalyze the first step in digestion of dietary starch and glycogen. The human genome has a cluster of several amylase genes that are expressed at high levels in either salivary gland or pancreas. This gene encodes an amylase isoenzyme produced by the pancreas. [provided by RefSeq, Jul 2008]

UniProt Comments for Amyalpha

AMY2A: Amylases are secreted proteins that hydrolyze 1,4-alpha-glucoside bonds in oligosaccharides and polysaccharides, and thus catalyze the first step in digestion of dietary starch and glycogen. The human genome has a cluster of several amylase genes that are expressed at high levels in either salivary gland or pancreas. This gene encodes an amylase isoenzyme produced by the pancreas. [provided by RefSeq, Jul 2008]

Protein type: Secreted; Secreted, signal peptide; EC 3.2.1.1; Carbohydrate Metabolism - starch and sucrose; Hydrolase

Chromosomal Location of Human Ortholog: 1p21

Cellular Component: extracellular space; extracellular region

Molecular Function: alpha-amylase activity; calcium ion binding; chloride ion binding

Biological Process: polysaccharide digestion; carbohydrate catabolic process; carbohydrate metabolic process; pathogenesis

Research Articles on Amyalpha

1. Mammalian pancreatic alpha-amylases share a common carbohydrate binding activity and specifically bind to the intestinal brush border.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with Amyalpha elisa kit

Products by Pathway	Pathway Diagram
Carbohydrate Digestion And Absorption Pathway antibodies	Carbohydrate Digestion And Absorption Pathway Diagram
Carbohydrate Digestion And Absorption Pathway antibodies	Carbohydrate Digestion And Absorption Pathway Diagram
Digestion Of Dietary Carbohydrate Pathway antibodies	Digestion Of Dietary Carbohydrate Pathway Diagram
Disease Pathway antibodies	Disease Pathway Diagram
Glycogen Storage Diseases Pathway antibodies	Glycogen Storage Diseases Pathway Diagram
Metabolism Pathway antibodies	Metabolism Pathway Diagram
Metabolism Of Carbohydrates Pathway antibodies	Metabolism Of Carbohydrates Pathway Diagram
Myoclonic Epilepsy Of Lafora Pathway antibodies	Myoclonic Epilepsy Of Lafora Pathway Diagram
Pancreatic Secretion Pathway antibodies	Pancreatic Secretion Pathway Diagram
Pancreatic Secretion Pathway antibodies	Pancreatic Secretion Pathway Diagram

Diseases associated with Amyalpha elisa kit

Disease Name	Pubmed Publications
Neoplasms Antibodies	>6 publications with Amyalpha and Neoplasms
Inflammation Antibodies	>2 publications with Amyalpha and Inflammation
Congenital Abnormalities Antibodies	>1 publications with Amyalpha and Congenital Abnormalities