Alpha2-Antiplasmin (SERPINF2) Antibody – Sheep Polyclonal

alpha2Antiplasmin (alpha2AP), also known as alpha2Plasmin Inhibitor (alpha2PI), is a member of the SERPIN family of proteinase inhibitors and the primary inhibitor of the enzyme plasmin in blood. It is produced in the liver and circulates in plasma at ~70 ug/ml (~1 uM). alpha2AP is a single chain molecule with a mass of 67 kDa as determined by SDS-PAGE. The primary target enzyme for alpha2AP is plasmin, but alpha2AP also acts as secondary or "backup" inhibitor of activated F.XI, activated Protein C and trypsin. Inhibition of these enzymes by alpha2AP occurs through proteolytic cleavage after Arg364 with subsequent rapid formation of a stable, inactive 1:1 enzyme- alpha2AP complex. alpha2AP also acts to regulate fibrinolysis by binding to the lysine binding sites on plasminogen thus competitively inhibiting plasminogen binding to fibrin. About 30% of alpha2AP present in plasma is partially degraded and lacks a peptide in the carboxyl region that contains the plasminogen-binding site. This form of alpha2AP (~65 kDa) has a reduced rate of plasmin inhibition and has been referred to as the "slow form" of alpha2AP. During fibrin formation, a portion of circulating alpha2AP is cross-linked to the alpha-chain of fibrin by activated factor XIII, and this linking of plasmin inhibitor to the plasmin substrate provides an additional measure of protection to the fibrin clot from proteolysis by plasmin1-4.

1. Aoki N, Suni Y, Miura O, Hirosawa S; Human alpha2Plasmin Inhibitor; Methods in Enzymology, 223, pp 185-197, 1993.
2. Shieh BH, Travis J; The Reactive Site of Human alpha2-Plasmin Inhibitor ; JBC 262, pp 6055-6059, 1987.
3. Moroi M, Aoki N; Isolation and Characterization of alpha2-Plasmin Inhibitor from Human Plasma; JBC 251, pp 5956-5965, 1976.
4. Harpel PC; Blood Proteolytic Enzyme Inhibitors: Their Role in Modulating Blood Coagulation and Fibrinolytic Enzyme Pathways; in Hemostasis and Thrombosis, eds. RW Colman, J Hirsh, VJ Marder and EW Salzman, pp. 738-747, J.B. Lippincott Co., Philadelphia PA, USA, 1982.

Small volumes of anti-Serpinf2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS512009 is an antibody targeting Serpinf2. Also known as Sheep anti-human alpha2Antiplasmin (alpha2AP), Whole IgG from antiserum. Host: Sheep.