ATG13 Recombinant Protein

Function: Autophagy factor required for autophagosome formation and mitophagy. Target of the TOR kinase signaling pathway that regulates autophagy through the control of the phosphorylation status of ATG13 and ULK1, and the regulation of the ATG13-ULK1-RB1CC1 complex. Through its regulation of ULK1 activity, plays a role in the regulation of the kinase activity of mTORC1 and cell proliferation. Ref.9 Ref.10 Ref.11 Ref.12 Ref.13 Ref.14

Subunit structure: Part of a complex consisting of ATG13, ULK1 and RB1CC1. Interacts with ATG101. Interacts with ULK1 (via C-terminus). Interacts with ULK2 (via C-terminus). Interacts (via the LIR motif) with GABARAP, GABARAPL, GABARAPL2, and LC3A. Interacts with TAB2 and TAB3. Ref.8 Ref.9 Ref.10 Ref.11 Ref.12 Ref.13 Ref.15 Ref.16 Ref.17

Subcellular location: Cytoplasm › cytosol

By similarity. Preautophagosomal structure

By similarity. Note: Under starvation conditions, is localized to puncate structures primarily representing the isolation membrane; the isolation membrane sequesters a portion of the cytoplasm resulting in autophagosome formation

By similarity.

Domain: The LIR motif (LC3-interacting region) is required for the interaction with the ATG8 family proteins GABARAP, GABARAPL, GABARAPL2, and LC3A. Ref.17

Post-translational modification: Phosphorylated by ULK1, ULK2 and mTOR. Phosphorylation status depends on nutrient-rich conditions; dephosphorylated during starvation or following treatment with rapamycin. ULK1-mediated phosphorylation of ATG13 at Ser-355 is required for efficient clearance of depolarized mitochondria. Ref.9 Ref.10 Ref.11 Ref.12 Ref.13 Ref.15

Sequence similarities: Belongs to the ATG13 metazoan family.

Sequence caution: The sequence BAA31627.2 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.The sequence BAD97323.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.