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CDC7 Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS515101

Recombinant full-length human CDC7 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.Scientific Background: CDC7 is a cell division cycle protein that is critical for the G1/S transition and initiation of DNA replication during the… More details

$350 / 0.02 mg +1 FREE 8GB USB
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Sample Manual Insert View PDF
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Testing Data View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
CDC7, Recombinant Protein
Full Product Name
CDC7 recombinant protein
Product Gene Name
Product Synonym Gene Name
CDC7L1; HsCDC7; Hsk1; huCDC7; MGC117361; MGC126237; MGC126238
Product Synonym Names
CDC7L1; HsCDC7; Hsk1; huCDC7; MGC117361; MGC126237; MGC126238
Host
Sf9 insect cells
Form/Format
50mM Tris-HCl, pH 7.5, 50mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
Preparation and Storage
Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Molecular Weight
~94 kDa
Chromosome Location
Chromosome: 1; NC_000001.10 (91966338..91991321). Location: 1p22
OMIM
603311
3D Structure
ModBase 3D Structure for O00311
Type
Recombinant Fusion Protein
Species
Human
Tag Information
GST tag
Expression System
Sf9 insect cells using baculovirus
Source Note
Recombinant full-length human CDC7 was expressed by baculovirus in Sf9 insect cells
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Applications Tested/Suitable for CDC7 recombinant protein
Western Blot (WB)

Product References and Citations for CDC7 recombinant protein

1. Montagnoli, A. et al: Targeting cell division cycle 7 kinase: a new approach for cancer therapy. Clin Cancer Res. 2010 Sep 15;16(18):4503-8.
2. Chuang, L C. et al: Phosphorylation of Mcm2 by Cdc7 promotes pre-replication complex assembly during cell-cycle re-entry. Mol Cell. 2009 Jul 31;35(2):206-16.

Other Notes

Small volumes of CDC7 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CDC7 purchase

MBS515101 is a recombinant protein targeting CDC7. Host: Sf9 insect cells.

Additional Details

NCBI/Uniprot data below describe general gene information for CDC7. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
Homo sapiens cell division cycle 7 (CDC7), transcript variant 1, mRNA
NCBI Official Synonym Full Names
cell division cycle 7
NCBI Official Symbol
CDC7
NCBI Official Synonym Symbols
Hsk1; CDC7L1; HsCDC7; huCDC7
UniProt Protein Name
Cell division cycle 7-related protein kinase
UniProt Gene Name
CDC7
UniProt Synonym Gene Names
CDC7L1; CDC7-related kinase; HsCdc7; huCdc7
UniProt Entry Name
CDC7_HUMAN
NCBI Protein Information
cell division cycle 7-related protein kinase; CDC7-related kinase; cell division cycle 7 homolog; cell division cycle 7-like protein 1; CDC7 (cell division cycle 7, S. cerevisiae, homolog)-like 1

NCBI Summary for CDC7

This gene encodes a cell division cycle protein with kinase activity that is critical for the G1/S transition. The yeast homolog is also essential for initiation of DNA replication as cell division occurs. Overexpression of this gene product may be associated with neoplastic transformation for some tumors. Multiple alternatively spliced transcript variants that encode the same protein have been detected. [provided by RefSeq, Aug 2008]
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UniProt Comments for CDC7

Function: Seems to phosphorylate critical substrates that regulate the G1/S phase transition and/or DNA replication. Can phosphorylates MCM2 and MCM3. Ref.9

Catalytic activity: ATP + a protein = ADP + a phosphoprotein.

Cofactor: Magnesium.

Subunit structure: Forms a complex with either DBF4/DBF4A or DBF4B, leading to the activation of the kinase activity.

Subcellular location: Nucleus.

Sequence similarities: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDC7 subfamily.Contains 1 protein kinase domain.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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