DLK Recombinant Protein

Name: DLK Recombinant Protein; Research Grade; MyBioSource
Brand: MyBioSource
SKU: MBS515116
Price: 340 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS515116

Recombinant human DLK (1-520) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.Scientific Background: DLK or mitogen-activated protein kinase kinase kinase 12 is a transmembrane protein containing six epidermal growth factor… More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Product Name

DLK, Recombinant Protein

Full Product Name

DLK recombinant protein

Product Gene Name

DLK recombinant protein

Product Synonym Gene Name

MAP3K12; MUK; ZPK; ZPKP1; MEKK12; MAP3K12

Product Synonym Names

MAP3K12; MUK; ZPK; ZPKP1; MEKK12; MAP3K12

Host

Sf9 insect cells

Form/Format

50mM Tris-HCl, pH 7.5, 50mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.

Preparation and Storage

Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Molecular Weight

~94 kDa

Sequence Positions

1-520

Chromosome Location

Chromosome: 12; NC_000012.11 (53873456..53893444, complement). Location: 12q13

OMIM

600447

3D Structure

ModBase 3D Structure for Q12852

Type

Recombinant Fusion Protein

Species

Human

Tag Information

GST tag

Expression System

Sf9 insect cells using baculovirus

Source Note

Recombinant human DLK (1-520) was expressed by baculovirus in Sf9 insect cells

Product Categories/Family for DLK recombinant protein

Cellular Proteins; Signaling Proteins - Cellular Proteins

Applications Tested/Suitable for DLK recombinant protein

Western Blot (WB)

Product References and Citations for DLK recombinant protein

1. Hirai, S. et al: Activation of the JNK pathway by distantly related protein kinases, MEKK and MUK. Oncogene 12: 641-650, 1996.
2. Ferron, S. R.et.al: Postnatal loss of Dlk1 imprinting in stem cells and niche astrocytes regulates neurogenesis. Nature 475: 381-385, 2011.

Other Notes

Small volumes of DLK recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for DLK purchase

MBS515116 is a recombinant protein targeting DLK. Host: Sf9 insect cells.

Additional Details

NCBI/Uniprot data below describe general gene information for DLK. It may not necessarily be applicable to this product.

NCBI GI #

301898340

NCBI GeneID

7786

NCBI Accession #

NP_006292.3

UniProt Primary Accession #

Q12852

UniProt Secondary Accession #

Q86VQ5; Q8WY25; B3KSS9; G3V1Y2

UniProt Related Accession #

Q12852

GenBank Nucleotide #

NM_006301

NCBI Official Full Name

Homo sapiens mitogen-activated protein kinase kinase kinase 12 (MAP3K12), transcript variant 2, mRNA

NCBI Official Synonym Full Names

mitogen-activated protein kinase kinase kinase 12

NCBI Official Symbol

MAP3K12

NCBI Official Synonym Symbols

DLK; MUK; ZPK; ZPKP1; MEKK12

UniProt Protein Name

Mitogen-activated protein kinase kinase kinase 12

UniProt Synonym Protein Names

Dual leucine zipper bearing kinase; DLK; Leucine-zipper protein kinase; ZPK; MAPK-upstream kinase; MUK; Mixed lineage kinase

Protein Family

Death-associated protein kinase

UniProt Gene Name

MAP3K12

UniProt Synonym Gene Names

ZPK; DLK; ZPK; MUK

UniProt Entry Name

M3K12_HUMAN

NCBI Protein Information

mitogen-activated protein kinase kinase kinase 12; protein kinase MUK; MAPK-upstream kinase; mixed lineage kinase; leucine zipper protein kinase; dual leucine zipper kinase DLK; dual leucine zipper bearing kinase

NCBI Summary for DLK

This gene encodes a member of the serine/threonine protein kinase family. This kinase contains a leucine-zipper domain and is predominately expressed in neuronal cells. The phosphorylation state of this kinase in synaptic terminals was shown to be regulated by membrane depolarization via calcineurin. This kinase forms heterodimers with leucine zipper containing transcription factors, such as cAMP responsive element binding protein (CREB) and MYC, and thus may play a regulatory role in PKA or retinoic acid induced neuronal differentiation. Alternatively spliced transcript variants encoding different proteins have been described.[provided by RefSeq, Jul 2010]

UniProt Comments for DLK

Function: May be an activator of the JNK/SAPK pathway. Phosphorylates beta-casein, histone 1 and myelin basic protein in vitro.

Catalytic activity: ATP + a protein = ADP + a phosphoprotein.

Cofactor: Magnesium.

Subunit structure: Interacts with MBIP. Ref.7

Subcellular location: Cytoplasm

By similarity. Membrane; Peripheral membrane protein

By similarity.

Tissue specificity: Highly expressed in brain and kidney.

Domain: Interacts with MBIP through the leucine-zipper motif.

Post-translational modification: Autophosphorylated on Ser/Thr. Phosphorylated in cytosol under basal conditions and dephosphorylated when membrane-associated

By similarity.

Sequence similarities: Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.Contains 1 protein kinase domain.

Research Articles on DLK

1. these findings indicate that DLK participates in cell proliferation and/or survival, at least in part, by modulating the expression of cell cycle regulatory proteins.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with DLK recombinant protein

Reference Product	PubMed Publications
MBIP recombinant protein	>1 publications with DLK and MBIP

Pathways associated with DLK recombinant protein

Products by Pathway	Pathway Diagram
Insulin Signaling Pathway antibodies	Insulin Signaling Pathway Diagram
MAPK Cascade Pathway antibodies	MAPK Cascade Pathway Diagram
MAPK Signaling Pathway antibodies	MAPK Signaling Pathway Diagram
MAPK Signaling Pathway antibodies	MAPK Signaling Pathway Diagram
MAPK Signaling Pathway antibodies	MAPK Signaling Pathway Diagram
Regulation Of P38-alpha And P38-beta Pathway antibodies	Regulation Of P38-alpha And P38-beta Pathway Diagram

Diseases associated with DLK recombinant protein

Disease Name	Pubmed Publications
Neoplasms Antibodies	>3 publications with DLK and Neoplasms

Organs/Tissues associated with DLK recombinant protein

Organ/Tissue Name	Pubmed Publications
Nerve Antibodies	>2 publications with DLK and Nerve
Brain Antibodies	>2 publications with DLK and Brain
Bone Antibodies	>1 publications with DLK and Bone
Bone Marrow Antibodies	>1 publications with DLK and Bone Marrow
Blood Antibodies	>1 publications with DLK and Blood
Kidney Antibodies	>1 publications with DLK and Kidney