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KDM5B Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS515123

Recombinant mouse KDM5B (1-753) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.Scientific Background: KDM5B is a lysine (K)-specific demethylase 5B which is highly expressed in testis and shows the lowest expression in skeletal… More details

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Product Overview

Online Datasheet in PDF
Product Name
KDM5B, Recombinant Protein
Full Product Name
KDM5B recombinant protein
Product Gene Name
KDM5B recombinant protein
Product Synonym Gene Name
Jarid1b;PUT1;Plu1;PLU-1;Rb-Bp2; AW556288; RBBP2H1A; mKIAA4034
Product Synonym Names
Jarid1b;PUT1;Plu1;PLU-1;Rb-Bp2; AW556288; RBBP2H1A; mKIAA4034
Host
Sf9 insect cells
Form/Format
50mM Tris-HCl, pH 7.5, 50mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
Preparation and Storage
Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Molecular Weight
~112 kDa
Sequence Positions
1-753
Chromosome Location
Chromosome: 1; NC_000067.6 (134560178..134632878). Location: 1 E4; 1 58.24 cM
3D Structure
ModBase 3D Structure for Q80Y84
Type
Recombinant Fusion Protein
Species
Mouse
Tag Information
GST tag
Expression System
Sf9 insect cells using baculovirus
Source Note
Recombinant mouse KDM5B (1-753) was expressed by baculovirus in Sf9 insect cells
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.

Product Categories/Family for KDM5B recombinant protein

Deacetylases/Demethylase Proteins; Signaling Proteins - Deacetylase/Demethylase Proteins
Applications Tested/Suitable for KDM5B recombinant protein
Western Blot (WB)

SDS-PAGE of KDM5B recombinant protein

KDM5B Recombinant Protein – Cat# MBS515123

Product References and Citations for KDM5B recombinant protein

1. Kashuba, V. et.al: Isolation and chromosomal localization of a new human retinoblastoma binding protein 2 homologue 1a (RBBP2H1A). Europ. J. Hum. Genet. 8: 407-413, 2000.
2. Xiang, Y. et.al: JARID1B is a histone H3 lysine 4 demethylase up-regulated in prostate cancer. Proc. Nat. Acad. Sci. 104: 19226-19231, 2007.

Other Notes

Small volumes of KDM5B recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for KDM5B purchase

MBS515123 is a recombinant protein targeting KDM5B. Host: Sf9 insect cells.

Additional Details

NCBI/Uniprot data below describe general gene information for KDM5B. It may not necessarily be applicable to this product.

NCBI GI #
34784160
NCBI GeneID
75605
NCBI Accession #
BC057318
UniProt Primary Accession #
Q80Y84
UniProt Secondary Accession #
Q5DTR9; Q8BLU1; Q8JZL8
UniProt Related Accession #
Q80Y84
NCBI Official Full Name
Mus musculus jumonji, AT rich interactive domain 1B (Rbp2 like), mRNA
NCBI Official Synonym Full Names
lysine (K)-specific demethylase 5B
NCBI Official Symbol
Kdm5b
NCBI Official Synonym Symbols
PUT1; Plu1; PLU-1; Rb-Bp2; Jarid1b; AW556288; RBBP2H1A; mKIAA4034; D1Ertd202e; 2010009J12Rik; 2210016I17Rik
UniProt Protein Name
Lysine-specific demethylase 5B
UniProt Synonym Protein Names
Histone demethylase JARID1B; Jumonji/ARID domain-containing protein 1B; PLU-1
Protein Family
Lysine-specific demethylase
UniProt Gene Name
Kdm5b
UniProt Synonym Gene Names
Jarid1b; Kiaa4034; Plu1
UniProt Entry Name
KDM5B_MOUSE
NCBI Protein Information
lysine-specific demethylase 5B; histone demethylase JARID1B; putative DNA/chromatin binding motif 1; jumonji/ARID domain-containing protein 1B; jumonji, AT rich interactive domain 1B (Rbp2 like)

UniProt Comments for KDM5B

Function: Histone demethylase that demethylates 'Lys-4' of histone H3, thereby playing a central role in histone code. Does not demethylate histone H3 'Lys-9' or H3 'Lys-27'. Demethylates trimethylated, dimethylated and monomethylated H3 'Lys-4'. Acts as a transcriptional corepressor for FOXG1B and PAX9. Ref.7 Ref.8

Cofactor: Binds 1 Fe2+ ion per subunit

By similarity.

Subunit structure: Interacts with FOXG1B, PAX9, MYC, MYCN and RB1. Interacts with HDAC1, HDAC4, HDAC5 and HDAC7

By similarity.

Subcellular location: Nucleus Ref.6.

Tissue specificity: Present at highest levels in testis, where it is enriched in spermatogonia and pachytene cells (at protein level). Ref.1 Ref.5 Ref.6

Developmental stage: Expressed in developing brain, mammary bud, thymus, teeth, whisker follicle, intervertebral disks, olfactory epithelium, eye, stomach and limbs. Ref.1

Domain: Both the JmjC domain and the JmjN domain are required for enzymatic activity.The 2 first PHD-type zinc finger domains are required for transcription repression activity

By similarity.

Sequence similarities: Belongs to the JARID1 histone demethylase family.Contains 1 ARID domain.Contains 1 JmjC domain.Contains 1 JmjN domain.Contains 3 PHD-type zinc fingers.

Sequence caution: The sequence BAD90482.1 differs from that shown. Reason: Erroneous initiation.
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Research Articles on KDM5B

1. Results suggest a role for Jarid1b in Rb-mediated repression of cell cycle genes during senescence.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Diseases associated with KDM5B recombinant protein

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >33 publications with KDM5B and Neoplasms
 Breast Neoplasms Antibodies  >14 publications with KDM5B and Breast Neoplasms
 Skin Neoplasms Antibodies  >3 publications with KDM5B and Skin Neoplasms
 Neoplasms, Experimental Antibodies  >2 publications with KDM5B and Neoplasms, Experimental
 Adenocarcinoma Antibodies  >2 publications with KDM5B and Adenocarcinoma
 Neovascularization, Pathologic Antibodies  >1 publications with KDM5B and Neovascularization, Pathologic
 Respiratory Tract Diseases Antibodies  >1 publications with KDM5B and Respiratory Tract Diseases
 Precancerous Conditions Antibodies  >1 publications with KDM5B and Precancerous Conditions
 Inflammation Antibodies  >1 publications with KDM5B and Inflammation

Organs/Tissues associated with KDM5B recombinant protein

 Organ/Tissue Name  Pubmed Publications
 Eye Antibodies  >4 publications with KDM5B and Eye
 Embryonic Tissue Antibodies  >3 publications with KDM5B and Embryonic Tissue
 Pancreas Antibodies  >1 publications with KDM5B and Pancreas
 Thymus Antibodies  >1 publications with KDM5B and Thymus
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