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CaMKPase Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS515128

Recombinant full-length human CaMKPase was expressed in E. coli cells using an N-terminal GST tag.Scientific Background: CaMKPase is a member of the PP2C family of Ser/Thr protein phosphatases that dephosphorylate and regulate the multifunctional… More details

$350 / 0.02 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
CaMKPase, Recombinant Protein
Full Product Name
CaMKPase recombinant protein
Product Synonym Gene Name
PPM1F; FEM-2; hFEM-2; KIAA0015; POPX2
Product Synonym Names
PPM1F; FEM-2; hFEM-2; KIAA0015; POPX2
Host
E Coli
Form/Format
50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
Preparation and Storage
Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Molecular Weight
~74 kDa
Chromosome Location
Chromosome: 22; NC_000022.10 (22273792..22307250, complement). Location: 22q11.22
3D Structure
ModBase 3D Structure for P49593
Type
Recombinant Fusion Protein
Species
Human
Tag Information
GST tag
Expression System
E.coli
Source Note
Recombinant full-length human CaMKPase was expressed in E. coli cells
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Applications Tested/Suitable for CaMKPase recombinant protein
Western Blot (WB)

Product References and Citations for CaMKPase recombinant protein

1. Isamu, K. et al: Phosphorylation and activation of Ca2+/calmodulin-dependent protein kinase phosphatase by Ca2+/calmodulin-dependent protein kinase II. FEBS Letters,1999; 456: 249-252.
2. Ishida, A. et al: Stimulation of Ca2+/calmodulin-dependent protein kinase phosphatase by polycations. Archives of Biochemistry and Biophysics, 2002; 408:229-238.

Other Notes

Small volumes of CaMKPase recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CaMKPase purchase

MBS515128 is a recombinant protein targeting CaMKPase. Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for CaMKPase. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
Homo sapiens protein phosphatase, Mg2+/Mn2+ dependent, 1F (PPM1F), mRNA
NCBI Official Synonym Full Names
protein phosphatase, Mg2+/Mn2+ dependent, 1F
NCBI Official Symbol
PPM1F
NCBI Official Synonym Symbols
CAMKP; FEM-2; POPX2; hFEM-2; CaMKPase
UniProt Protein Name
Protein phosphatase 1F
UniProt Synonym Protein Names
Ca(2+)/calmodulin-dependent protein kinase phosphatase; CaM-kinase phosphatase; CaMKPase; Partner of PIX 2; Protein fem-2 homolog
UniProt Gene Name
PPM1F
UniProt Synonym Gene Names
KIAA0015; POPX2; CaM-kinase phosphatase; CaMKPase; hFem-2
UniProt Entry Name
PPM1F_HUMAN
NCBI Protein Information
protein phosphatase 1F; PP2C phosphatase; partner of PIX 2; protein fem-2 homolog; CaM-kinase phosphatase; protein phosphatase 1F (PP2C domain containing); Ca(2+)/calmodulin-dependent protein kinase phosphatase

NCBI Summary for CaMKPase

The protein encoded by this gene is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase can interact with Rho guanine nucleotide exchange factors (PIX), and thus block the effects of p21-activated kinase 1 (PAK), a protein kinase mediating biological effects downstream of Rho GTPases. Calcium/calmodulin-dependent protein kinase II gamma (CAMK2G/CAMK-II) is found to be one of the substrates of this phosphatase. The overexpression of this phosphatase or CAMK2G has been shown to mediate caspase-dependent apoptosis. An alternatively spliced transcript variant has been identified, but its full-length nature has not been determined. [provided by RefSeq, Jul 2008]
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UniProt Comments for CaMKPase

Function: Dephosphorylates and concomitantly deactivates CaM-kinase II activated upon autophosphorylation, and CaM-kinases IV and I activated upon phosphorylation by CaM-kinase kinase. Promotes apoptosis.

Catalytic activity: A phosphoprotein + H2O = a protein + phosphate.

Cofactor: Binds 2 magnesium or manganese ions per subunit

By similarity.

Subunit structure: Associates with FEM1B.

Sequence similarities: Belongs to the PP2C family.

Sequence caution: The sequence BAA02803.2 differs from that shown. Reason: Erroneous initiation.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Diseases associated with CaMKPase recombinant protein

Organs/Tissues associated with CaMKPase recombinant protein

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