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CREB1 Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS515142

Recombinant rat CREB1 (1-280) was expressed in E. coli cells using a N-terminal GST tag.Scientific Background: CREB1 is a member of the cAMP responsive element binding protein family of transcription factors that are critical mediators of gene expression in… More details

$370 / 0.02 mg +1 FREE 8GB USB
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Product Overview

Online Datasheet in PDF
Product Name
CREB1, Recombinant Protein
Full Product Name
CREB1 recombinant protein
Product Gene Name
CREB1 recombinant protein
Product Synonym Gene Name
Creb
Product Synonym Names
cAMP Responsive Element Binding Protein 1; creb; CREB1 Protein
Host
E Coli
Form/Format
50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
Preparation and Storage
Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Molecular Weight
~59 kDa
Sequence Positions
1-280
Chromosome Location
Chromosome: 9; NC_005108.3 (71282730..71345853, complement). Location: 9q32
3D Structure
ModBase 3D Structure for P15337
Type
Recombinant Fusion Protein
Species
Rat
Tag Information
GST tag
Expression System
E.coli
Source Note
Recombinant rat CREB1 (1-280) was expressed in E.coli cells
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.

Product Categories/Family for CREB1 recombinant protein

Transcription Proteins; Signaling Proteins - Transcription Proteins
Applications Tested/Suitable for CREB1 recombinant protein
Kinase Assay, Western Blot (WB)

SDS-PAGE of CREB1 recombinant protein

CREB1 Recombinant Protein – Cat# MBS515142

Product References and Citations for CREB1 recombinant protein

1. Valverde, O. et al: Modulation of anxiety-like behavior and morphine dependence in CREB-deficient mice. Neuropsychopharmacology. 2004 Jun; 29(6): 1122-33.
2. Mantamadiotis, T. et al: Disruption of CREB function in brain leads to neurodegeneration. Nat Genet. 2002 May; 31(1): 47-54.

Other Notes

Small volumes of CREB1 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CREB1 purchase

MBS515142 is a recombinant protein targeting CREB1. Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for CREB1. It may not necessarily be applicable to this product.

NCBI GI #
13591923
NCBI GeneID
81646
NCBI Accession #
NP_112279.1
UniProt Primary Accession #
P15337
UniProt Related Accession #
P15337
GenBank Nucleotide #
NM_031017
NCBI Official Full Name
Rattus norvegicus cAMP responsive element binding protein 1 (Creb1), transcript variant B, mRNA
NCBI Official Synonym Full Names
cAMP responsive element binding protein 1
NCBI Official Symbol
Creb1
NCBI Official Synonym Symbols
Creb
UniProt Protein Name
Cyclic AMP-responsive element-binding protein 1
Protein Family
Cyclic AMP-responsive element-binding protein
UniProt Gene Name
Creb1
UniProt Synonym Gene Names
Creb-1; CREB-1
UniProt Entry Name
CREB1_RAT
NCBI Protein Information
cyclic AMP-responsive element-binding protein 1; CREB-1; Y protein; cAMP response element binding protein 1; cAMP-responsive element-binding protein 1

NCBI Summary for CREB1

binds the cAMP response element in many gene promoters and regulates transcription [RGD, Feb 2006]

UniProt Comments for CREB1

Function: Phosphorylation-dependent transcription factor that stimulates transcription upon binding to the DNA cAMP response element (CRE), a sequence present in many viral and cellular promoters. Transcription activation is enhanced by the TORC coactivators which act independently of Ser-117 phosphorylation. Involved in different cellular processes including the synchronization of circadian rhythmicity and the differentiation of adipose cells

By similarity.

Subunit structure: Interacts with PPRC1. Binds DNA as a dimer. Interacts, through the bZIP domain, with the coactivators TORC1/CRTC1, TORC2/CRTC2 and TORC3/CRTC3

By similarity. When phosphorylated on Ser-133, binds CREBBP. Interacts with ARRB1. Interacts (phosphorylated form) with TOX3. Binds to HIPK2

By similarity. Interacts with SGK1

By similarity. Interacts with CREBL2; regulates CREB1 phosphorylation, stability and transcriptional activity

By similarity.

Subcellular location: Nucleus.

Post-translational modification: Phosphorylation of Ser-133 allows CREBBP binding. Stimulated by phosphorylation. Phosphorylation of both Ser-142 and Ser-133 in the SCN regulates the activity of CREB and participate in circadian rhythm generation

By similarity. Phosphorylated upon calcium influx by CaMK4 and CaMK2 on Ser-133. CaMK4 is much more potent than CaMK2 in activating CREB. Phosphorylated by CaMK2 on Ser-142. Phosphorylation of Ser-142 blocks CREB-mediated transcription even when Ser-133 is phosphorylated. Phosphorylated by CaMK1. Phosphorylation of Ser-271 by HIPK2 in response to genotoxic stress promotes CREB1 activity, facilitating the recruitment of the coactivator CBP. Phosphorylated at Ser-133 by RPS6KA3, RPS6KA4 and RPS6KA5 in response to mitogenic or stress stimuli

By similarity. CREBL2 positively regulates phosphorylation at Ser-133 thereby stimulating CREB1 transcriptional activity

By similarity. Ref.3 Ref.4 Ref.5Sumoylated with SUMO1. Sumoylation on Lys-304, but not on Lys-285, is required for nuclear localization of this protein. Sumoylation is enhanced under hypoxia, promoting nuclear localization and stabilization

By similarity.

Sequence similarities: Belongs to the bZIP family.Contains 1 bZIP (basic-leucine zipper) domain.Contains 1 KID (kinase-inducible) domain.
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Research Articles on CREB1

1. These results suggest that Y1R participates in the reciprocal regulation of NPY, glutathione peroxidase, and CREB in the hypothalamus during phenylpropanolamine treatment in conscious rats.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with CREB1 recombinant protein

 Reference Product  PubMed Publications
 Fos recombinant protein  >45 publications with CREB1 and Fos
 Mapk1 recombinant protein  >34 publications with CREB1 and Mapk1
 Mapk3 recombinant protein  >33 publications with CREB1 and Mapk3
 Crebbp recombinant protein  >18 publications with CREB1 and Crebbp
 Ep300 recombinant protein  >6 publications with CREB1 and Ep300
 Akt1 recombinant protein  >6 publications with CREB1 and Akt1
 Camk4 recombinant protein  >5 publications with CREB1 and Camk4
 Ciita recombinant protein  >2 publications with CREB1 and Ciita

Pathways associated with CREB1 recombinant protein

 Products by Pathway  Pathway Diagram
 AKT Phosphorylates Targets In The Nucleus Pathway antibodies  AKT Phosphorylates Targets In The Nucleus Pathway Diagram
 Activated TLR4 Signalling Pathway antibodies  Activated TLR4 Signalling Pathway Diagram
 Activation Of NMDA Receptor Upon Glutamate Binding And Postsynaptic Events Pathway antibodies  Activation Of NMDA Receptor Upon Glutamate Binding And Postsynaptic Events Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 Adipogenesis Pathway antibodies  Adipogenesis Pathway Diagram
 Alcoholism Pathway antibodies  Alcoholism Pathway Diagram
 Alcoholism Pathway antibodies  Alcoholism Pathway Diagram
 Amphetamine Addiction Pathway antibodies  Amphetamine Addiction Pathway Diagram
 Amphetamine Addiction Pathway antibodies  Amphetamine Addiction Pathway Diagram
 Antigen Processing And Presentation Pathway antibodies  Antigen Processing And Presentation Pathway Diagram

Diseases associated with CREB1 recombinant protein

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >173 publications with CREB1 and Neoplasms
 Nervous System Diseases Antibodies  >90 publications with CREB1 and Nervous System Diseases
 Inflammation Antibodies  >57 publications with CREB1 and Inflammation
 Depressive Disorder Antibodies  >38 publications with CREB1 and Depressive Disorder
 Memory Disorders Antibodies  >37 publications with CREB1 and Memory Disorders
 Necrosis Antibodies  >36 publications with CREB1 and Necrosis
 Learning Disorders Antibodies  >29 publications with CREB1 and Learning Disorders
 Cognition Disorders Antibodies  >15 publications with CREB1 and Cognition Disorders
 Liver Diseases Antibodies  >14 publications with CREB1 and Liver Diseases
 Fibrosis Antibodies  >12 publications with CREB1 and Fibrosis

Organs/Tissues associated with CREB1 recombinant protein

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >241 publications with CREB1 and Brain
 Heart Antibodies  >29 publications with CREB1 and Heart
 Embryonic Tissue Antibodies  >20 publications with CREB1 and Embryonic Tissue
 Pancreas Antibodies  >12 publications with CREB1 and Pancreas
 Testis Antibodies  >9 publications with CREB1 and Testis
 Thymus Antibodies  >4 publications with CREB1 and Thymus
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