PHLPP2 Recombinant Protein

Function: Protein phosphatase that mediates dephosphorylation of 'Ser-473' of AKT1, 'Ser-660' of PRKCB isoform beta-II and 'Ser-657' of PRKCA. AKT1 regulates the balance between cell survival and apoptosis through a cascade that primarily alters the function of transcription factors that regulate pro- and antiapoptotic genes. Dephosphorylation of 'Ser-473' of AKT1 triggers apoptosis and decreases cell proliferation. Also controls the phosphorylation of AKT3. Dephosphorylation of PRKCA and PRKCB leads to their destabilization and degradation. Inhibits cancer cell proliferation and may act as a tumor suppressor. Ref.7 Ref.8 Ref.9

Catalytic activity: A phosphoprotein + H2O = a protein + phosphate.

Cofactor: Binds 2 manganese ions per subunit

By similarity.

Subunit structure: Interacts with AKT1, AKT3 and PRKCB isoform beta-II. Ref.7 Ref.8

Subcellular location: Cytoplasm. Membrane; Peripheral membrane protein. Nucleus. Note: In colorectal cancer tissue, expression is concentrated in the cytoplasm and nucleus. Ref.7 Ref.9

Tissue specificity: In colorectal cancer tissue, expression is highest in the surface epithelium of normal colonic mucosa adjacent to the cancer tissue but is largely excluded from the crypt bases. Expression is lost or significantly decreased in 80% of tested tumors (at protein level). Ref.9

Sequence similarities: Contains 22 LRR (leucine-rich) repeats.Contains 1 PH domain.Contains 1 PP2C-like domain.

Biophysicochemical propertiesKinetic parameters:KM=4.13 mM for p-nitrophenyl phosphate

Sequence caution: The sequence BAA76775.2 differs from that shown. Reason: Erroneous initiation. The sequence BAA91943.1 differs from that shown. Reason: Erroneous initiation.