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PI3K (p55 gamma) Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS515204

Recombinant full length human PI3K (p55 gamma) was expressed by baculovirus in Sf9 insect cells using an N-terminal His tag.Scientific Background: PI3K (p55 gamma) also known as PI3K regulatory subunit 3 (PIK3R3) was first identified by 2-hybrid system as a… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
PI3K (p55 gamma), Recombinant Protein
Full Product Name
PI3K (p55 gamma) recombinant protein
Product Synonym Gene Name
PIK3R3; p55; p55-GAMMA; FLJ41892; DKFZp686P05226
Product Synonym Names
PIK3R3; p55; p55-GAMMA; FLJ41892; DKFZp686P05226; PI3K (p55gamma) Protein; PI3K (p55g) Protein
Host
Insect cells
Form/Format
50mM sodium phosphate, pH 7.0, 300mM NaCl, 150mM imidazole, 0.1mM PMSF, 0.25mM DTT, 25% glycerol.
Preparation and Storage
Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Molecular Weight
~61 kDa
Chromosome Location
Chromosome: 1; NC_000001.10 (46505812..46642167, complement). Location: 1p34.1
OMIM
606076
3D Structure
ModBase 3D Structure for Q92569
Type
Recombinant Fusion Protein
Species
Human
Tag Information
His tag
Expression System
Sf9 insect cells using baculovirus
Source Note
Recombinant full length human PI3K (p55 gamma) was expressed in insect cells
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Applications Tested/Suitable for PI3K (p55 gamma) recombinant protein
Western Blot (WB)

Product References and Citations for PI3K (p55 gamma) recombinant protein

1. Dey, B R. et al: Cloning of human p55-gamma, a regulatory subunit of phosphatidylinositol 3-kinase, by a yeast two-hybrid library screen with the insulin-like growth factor-I receptor. Gene 209: 175-183, 1998.


Other Notes

Small volumes of PI3K (p55 gamma) recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PI3K (p55 gamma) purchase

MBS515204 is a recombinant protein targeting PI3K (p55 gamma). Host: Insect cells.

Additional Details

NCBI/Uniprot data below describe general gene information for PI3K (p55 gamma). It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
Homo sapiens phosphoinositide-3-kinase, regulatory subunit 3 (gamma), mRNA
NCBI Official Synonym Full Names
phosphoinositide-3-kinase, regulatory subunit 3 (gamma)
NCBI Official Symbol
PIK3R3
NCBI Official Synonym Symbols
p55; p55-GAMMA
UniProt Protein Name
Phosphatidylinositol 3-kinase regulatory subunit gamma
UniProt Synonym Protein Names
Phosphatidylinositol 3-kinase 55 kDa regulatory subunit gamma; PI3-kinase subunit p55-gamma; PtdIns-3-kinase regulatory subunit p55-gamma; p55PIK
UniProt Gene Name
PIK3R3
UniProt Synonym Gene Names
PI3-kinase regulatory subunit gamma; PI3K regulatory subunit gamma; PtdIns-3-kinase regulatory subunit gamma; PI3-kinase subunit p55-gamma
UniProt Entry Name
P55G_HUMAN
NCBI Protein Information
phosphatidylinositol 3-kinase regulatory subunit gamma; p55PIK; PI3-kinase subunit p55-gamma; PI3K regulatory subunit gamma; 100% homology to SWISS-PROT Q92569; PI3-kinase regulatory subunit gamma; ptdIns-3-kinase regulatory subunit gamma; ptdIns-3-kinase regulatory subunit p55-gamma; phosphoinositide-3-kinase, regulatory subunit 3 (p55, gamma); phosphatidylinositol 3-kinase 55 kDa regulatory subunit gamma; phosphoinositide-3-kinase, regulatory subunit, polypeptide 3 (p55, gamma); phosphatidylinositol 3-kinase, regulatory subunit, polypeptide 3 (p55, gamma)

UniProt Comments for PI3K (p55 gamma)

Function: Binds to activated (phosphorylated) protein-tyrosine kinases through its SH2 domain and regulates their kinase activity. During insulin stimulation, it also binds to IRS-1.

Subunit structure: Heterodimer of a regulatory subunit PIK3R3 and a p110 catalytic subunit (PIK3CA, PIK3CB or PIK3CD). Interacts with AXL. Ref.6

Tissue specificity: Highest levels in brain and testis. Lower levels in adipose tissue, kidney, heart, lung and skeletal muscle.

Sequence similarities: Belongs to the PI3K p85 subunit family.Contains 2 SH2 domains.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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