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PRKAR1B Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS515213

Recombinant full-length human PRKAR1B was expressed in Sf9 insect cells using a C-terminal His tag.Scientific Background: PRKAR1B is the type I-beta regulatory subunit of cyclic AMP-dependent protein kinase A (PKA) which is an essential enzyme in the cAMP… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
PRKAR1B, Recombinant Protein
Full Product Name
PRKAR1B recombinant protein
Product Gene Name
PRKAR1B recombinant protein
Product Synonym Gene Name
PRKAR1
Product Synonym Names
PRKAR1
Host
Sf9 insect cells
Form/Format
50mM sodium phosphate, pH 7.0, 300mM NaCl, 150mM imidazole, 0.1mM PMSF, 0.25mM DTT, 25% glycerol.
Preparation and Storage
Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Molecular Weight
~52 kDa
Chromosome Location
Chromosome: 7; NC_000007.13 (588834..767313, complement). Location: 7p22
OMIM
176911
3D Structure
ModBase 3D Structure for P31321
Type
Recombinant Fusion Protein
Species
Human
Tag Information
His tag
Expression System
Sf9 insect cells using baculovirus
Source Note
Recombinant full-length human PRKAR1B was expressed in Sf9 insect cells
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.

Product Categories/Family for PRKAR1B recombinant protein

Cellular Proteins; Signaling Proteins - Cellular Proteins
Applications Tested/Suitable for PRKAR1B recombinant protein
Western Blot (WB)

SDS-PAGE of PRKAR1B recombinant protein

PRKAR1B Recombinant Protein – Cat# MBS515213

Product References and Citations for PRKAR1B recombinant protein

1. Gullingsrud J, et al. Dynamic binding of PKA regulatory subunit RI alpha.Structure, 2006 Jan. PMID 16407073.
2. Carlson, C R. et al: A kinase anchoring protein (AKAP) interaction and dimerization of the RIalpha and RIbeta regulatory subunits of protein kinase a in vivo by the yeast two hybrid system. J Mol Biol. 2003 Mar 28;327(3):609-18.

Other Notes

Small volumes of PRKAR1B recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PRKAR1B purchase

MBS515213 is a recombinant protein targeting PRKAR1B. Host: Sf9 insect cells.

Additional Details

NCBI/Uniprot data below describe general gene information for PRKAR1B. It may not necessarily be applicable to this product.

NCBI GI #
258613967
NCBI GeneID
5575
NCBI Accession #
NP_002726.1
UniProt Primary Accession #
P31321
UniProt Secondary Accession #
Q8N422
UniProt Related Accession #
P31321
GenBank Nucleotide #
NM_002735
NCBI Official Full Name
Homo sapiens protein kinase, cAMP-dependent, regulatory, type I, beta (PRKAR1B), transcript variant 2, mRNA
NCBI Official Synonym Full Names
protein kinase, cAMP-dependent, regulatory, type I, beta
NCBI Official Symbol
PRKAR1B
NCBI Official Synonym Symbols
PRKAR1
UniProt Protein Name
cAMP-dependent protein kinase type I-beta regulatory subunit
Protein Family
cAMP-dependent protein kinase
UniProt Gene Name
PRKAR1B
UniProt Entry Name
KAP1_HUMAN
NCBI Protein Information
cAMP-dependent protein kinase type I-beta regulatory subunit

NCBI Summary for PRKAR1B

Cyclic AMP-dependent protein kinase A (PKA) is an essential enzyme in the signaling pathway of the second messenger cAMP. Through phosphorylation of target proteins, PKA controls many biochemical events in the cell including regulation of metabolism, ion transport, and gene transcription. The PKA holoenzyme is composed of 2 regulatory and 2 catalytic subunits and dissociates from the regulatory subunits upon binding of cAMP.[supplied by OMIM, Jun 2009]
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UniProt Comments for PRKAR1B

Function: Regulatory subunit of the cAMP-dependent protein kinases involved in cAMP signaling in cells. Ref.9

Subunit structure: The inactive holoenzyme is composed of two regulatory chains and two catalytic chains. Activation by cAMP releases the two active catalytic monomers and the regulatory dimer. Interacts with PRKX; regulates this cAMP-dependent protein kinase. Interacts with C2orf88/smAKAP; this interaction may target PRKAR1B to the plasma membrane. Ref.9 Ref.10

Subcellular location: Cell membrane Ref.10.

Tissue specificity: Four types of regulatory chains are found: I-alpha, I-beta, II-alpha, and II-beta. Their expression varies among tissues and is in some cases constitutive and in others inducible.

Post-translational modification: The pseudophosphorylation site binds to the substrate-binding region of the catalytic chain, resulting in the inhibition of its activity.

Sequence similarities: Belongs to the cAMP-dependent kinase regulatory chain family.Contains 2 cyclic nucleotide-binding domains.
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Research Articles on PRKAR1B

1. Observational study of gene-disease association. (HuGE Navigator)

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with PRKAR1B recombinant protein

 Reference Product  PubMed Publications
 PRKAR1A recombinant protein  >15 publications with PRKAR1B and PRKAR1A
 PRKAR2A recombinant protein  >8 publications with PRKAR1B and PRKAR2A
 PRKAR2B recombinant protein  >6 publications with PRKAR1B and PRKAR2B
 AKAP1 recombinant protein  >1 publications with PRKAR1B and AKAP1

Pathways associated with PRKAR1B recombinant protein

 Products by Pathway  Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 Aquaporin-mediated Transport Pathway antibodies  Aquaporin-mediated Transport Pathway Diagram
 Ca-dependent Events Pathway antibodies  Ca-dependent Events Pathway Diagram
 CaM Pathway antibodies  CaM Pathway Diagram
 Calcium Regulation In The Cardiac Cell Pathway antibodies  Calcium Regulation In The Cardiac Cell Pathway Diagram
 Calmodulin Induced Events Pathway antibodies  Calmodulin Induced Events Pathway Diagram
 DAG And IP3 Signaling Pathway antibodies  DAG And IP3 Signaling Pathway Diagram
 DAP12 Interactions Pathway antibodies  DAP12 Interactions Pathway Diagram
 DAP12 Signaling Pathway antibodies  DAP12 Signaling Pathway Diagram

Diseases associated with PRKAR1B recombinant protein

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >5 publications with PRKAR1B and Neoplasms
 Nervous System Diseases Antibodies  >2 publications with PRKAR1B and Nervous System Diseases
 Hyperplasia Antibodies  >1 publications with PRKAR1B and Hyperplasia
 Inflammation Antibodies  >1 publications with PRKAR1B and Inflammation

Organs/Tissues associated with PRKAR1B recombinant protein

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >8 publications with PRKAR1B and Brain
 Blood Antibodies  >7 publications with PRKAR1B and Blood
 Nerve Antibodies  >3 publications with PRKAR1B and Nerve
 Muscle Antibodies  >2 publications with PRKAR1B and Muscle
 Adrenal Gland Antibodies  >1 publications with PRKAR1B and Adrenal Gland
 Eye Antibodies  >1 publications with PRKAR1B and Eye
 Intestine Antibodies  >1 publications with PRKAR1B and Intestine
 Placenta Antibodies  >1 publications with PRKAR1B and Placenta
 Embryonic Tissue Antibodies  >1 publications with PRKAR1B and Embryonic Tissue
 Uterus Antibodies  >1 publications with PRKAR1B and Uterus
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