UCHL5 Recombinant Protein

Name: UCHL5 Recombinant Protein; Research Grade; MyBioSource
Brand: MyBioSource
SKU: MBS515258
Price: 250 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS515258

Recombinant full length human UCHL5 was expressed in E. coli cells using a N-terminal His tag.Scientific Background: UCHL5 or ubiquitin carboxyl-terminal hydrolase L5 is a member of the UCH enzyme family which contains a conserved catalytic domain and a… More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Product Name

UCHL5, Recombinant Protein

Full Product Name

UCHL5 recombinant protein

Product Gene Name

UCHL5 recombinant protein

Product Synonym Gene Name

CGI-70; INO80R; UCH-L5; UCH37

Product Synonym Names

CGI-70; INO80R; UCH-L5; UCH37

Host

E Coli

Form/Format

50mM sodium phosphate, pH 7.0, 300mM NaCl, 150mM imidazole, 0.1mM PMSF, 0.25mM DTT, 25% glycerol.

Preparation and Storage

Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Molecular Weight

~38 kDa

Chromosome Location

Chromosome: 1; NC_000001.10 (192981496..193029237, complement). Location: 1q32

OMIM

610667

3D Structure

ModBase 3D Structure for Q9Y5K5

Type

Recombinant Fusion Protein

Species

Human

Tag Information

His tag

Expression System

E.coli

Source Note

Recombinant full length human UCHL5 was expressed in E. coli cells

Product Categories/Family for UCHL5 recombinant protein

Ubiquitin Proteins; Signaling Proteins - Ubiquitin Proteins

Applications Tested/Suitable for UCHL5 recombinant protein

Western Blot (WB)

Product References and Citations for UCHL5 recombinant protein

1. Lam, Y. A. et.al: Editing of ubiquitin conjugates by an isopeptidase in the 26S proteasome. Nature 385: 737-740, 1997.
2. Wicks, S. J. et.al: The deubiquitinating enzyme UCH37 interacts with Smads and regulates TGF-beta signalling. Oncogene 24: 8080-8084, 2005.

Other Notes

Small volumes of UCHL5 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for UCHL5 purchase

MBS515258 is a recombinant protein targeting UCHL5. Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for UCHL5. It may not necessarily be applicable to this product.

NCBI GI #

312922358

NCBI GeneID

51377

NCBI Accession #

NP_001186190.1

UniProt Primary Accession #

Q9Y5K5

UniProt Secondary Accession #

Q5LJA6; Q5LJA7; Q8TBS4; Q96BJ9; Q9H1W5; Q9P0I3; Q9UQN2

UniProt Related Accession #

Q9Y5K5

GenBank Nucleotide #

NM_001199261

NCBI Official Full Name

Homo sapiens ubiquitin carboxyl-terminal hydrolase L5 (UCHL5), transcript variant 2, mRNA

NCBI Official Synonym Full Names

ubiquitin carboxyl-terminal hydrolase L5

NCBI Official Symbol

UCHL5

NCBI Official Synonym Symbols

UCH37; CGI-70; INO80R; UCH-L5

UniProt Protein Name

Ubiquitin carboxyl-terminal hydrolase isozyme L5

UniProt Synonym Protein Names

Ubiquitin C-terminal hydrolase UCH37; Ubiquitin thioesterase L5

Protein Family

Ubiquitin carboxyl-terminal hydrolase

UniProt Gene Name

UCHL5

UniProt Synonym Gene Names

UCH37; UCH-L5

UniProt Entry Name

UCHL5_HUMAN

NCBI Protein Information

ubiquitin carboxyl-terminal hydrolase isozyme L5; INO80 complex subunit R; ubiquitin thioesterase L5; ubiquitin C-terminal hydrolase UCH37; ubiquitin carboxyl-terminal esterase L5

UniProt Comments for UCHL5

Function: Protease that specifically cleaves 'Lys-48'-linked polyubiquitin chains. Deubiquitinating enzyme associated with the 19S regulatory subunit of the 26S proteasome. Putative regulatory component of the INO80 complex; however is inactive in the INO80 complex and is activated by a transient interaction of the INO80 complex with the proteasome via ADRM1. Ref.1 Ref.10

Catalytic activity: Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Enzyme regulation: Activated by ADRM1. Inhibited by interaction with NFRKB. Ref.1 Ref.8 Ref.10

Subunit structure: Component of the 19S (PA700) regulatory complex of the 26S proteasome. Interacts with ADRM1 and NFRKB; in vitro ADRM1 and NFRKB compete for interaction with UCHL5. Component of the INO80 complex; specifically part of a complex module associated with N-terminus of INO80. Ref.1 Ref.7 Ref.8 Ref.10 Ref.13

Subcellular location: Cytoplasm. Nucleus. Note: Associates with the proteasome 19S subunit in the cytoplasm. Associates with the INO80 complex in the nucleus. Ref.10

Sequence similarities: Belongs to the peptidase C12 family.

Research Articles on UCHL5

1. UCH37 over-expression is associated with hepatocellular carcinoma recurrence.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with UCHL5 recombinant protein

Reference Product	PubMed Publications
ADRM1 recombinant protein	>8 publications with UCHL5 and ADRM1

Pathways associated with UCHL5 recombinant protein

Products by Pathway	Pathway Diagram
Downregulation Of TGF-beta Receptor Signaling Pathway antibodies	Downregulation Of TGF-beta Receptor Signaling Pathway Diagram
Signal Transduction Pathway antibodies	Signal Transduction Pathway Diagram
Signaling By TGF-beta Receptor Complex Pathway antibodies	Signaling By TGF-beta Receptor Complex Pathway Diagram
TGF-beta Receptor Signaling Activates SMADs Pathway antibodies	TGF-beta Receptor Signaling Activates SMADs Pathway Diagram

Diseases associated with UCHL5 recombinant protein

Disease Name	Pubmed Publications
Neoplasms Antibodies	>11 publications with UCHL5 and Neoplasms
Adenocarcinoma Antibodies	>3 publications with UCHL5 and Adenocarcinoma
Carcinoma, Hepatocellular Antibodies	>2 publications with UCHL5 and Carcinoma, Hepatocellular
Inflammation Antibodies	>2 publications with UCHL5 and Inflammation
Carcinoma, Squamous Cell Antibodies	>2 publications with UCHL5 and Carcinoma, Squamous Cell
Liver Neoplasms Antibodies	>2 publications with UCHL5 and Liver Neoplasms
Neoplasm Metastasis Antibodies	>2 publications with UCHL5 and Neoplasm Metastasis
Breast Neoplasms Antibodies	>1 publications with UCHL5 and Breast Neoplasms
Gastrointestinal Diseases Antibodies	>1 publications with UCHL5 and Gastrointestinal Diseases
Esophageal Neoplasms Antibodies	>1 publications with UCHL5 and Esophageal Neoplasms

Organs/Tissues associated with UCHL5 recombinant protein

Organ/Tissue Name	Pubmed Publications
Liver Antibodies	>3 publications with UCHL5 and Liver
Kidney Antibodies	>2 publications with UCHL5 and Kidney
Cervix Antibodies	>1 publications with UCHL5 and Cervix
Blood Antibodies	>1 publications with UCHL5 and Blood
Lymph Node Antibodies	>1 publications with UCHL5 and Lymph Node
Uterus Antibodies	>1 publications with UCHL5 and Uterus
Lung Antibodies	>1 publications with UCHL5 and Lung
Skin Antibodies	>1 publications with UCHL5 and Skin
Spleen Antibodies	>1 publications with UCHL5 and Spleen
Lymph Antibodies	>1 publications with UCHL5 and Lymph