PTK9 Recombinant Protein

This gene encodes twinfilin, an actin monomer-binding protein conserved from yeast to mammals. Studies of the mouse counterpart suggest that this protein may be an actin monomer-binding protein, and its localization to cortical G-actin-rich structures may be regulated by the small GTPase RAC1. [provided by RefSeq, Jul 2008]

Function: Actin-binding protein involved in motile and morphological processes. Inhibits actin polymerization, likely by sequestering G-actin. By capping the barbed ends of filaments, it also regulates motility. Seems to play an important role in clathrin-mediated endocytosis and distribution of endocytic organelles

By similarity.

Subunit structure: Interacts with G-actin; ADP-actin form and capping protein (CP). May also be able to interact with TWF2 and phosphoinositides, PI(4,5)P2. When bound to PI(4,5)P2, it is down-regulated

By similarity.

Subcellular location: Cytoplasm. Cytoplasm › cytoskeleton

By similarity. Note: Diffuse cytoplasmic localization with perinuclear and G-actin-rich cortical actin structures sublocalization. Also found at membrane ruffles and cell-cell contacts

By similarity.

Tissue specificity: Expressed at high levels in the colon, testis, ovary, prostate and lung. Expressed at lower levels in the brain, bladder and heart. Not detected in liver. Ref.1

Post-translational modification: Phosphorylated on serine and threonine residues. Ref.1

Sequence similarities: Belongs to the actin-binding proteins ADF family. Twinfilin subfamily.Contains 2 ADF-H domains.

Caution: Was originally (Ref.1) thought to have protein tyrosine kinase activity.

Sequence caution: The sequence AAH43148.2 differs from that shown. Reason: Erroneous initiation. The sequence AAH68548.1 differs from that shown. Reason: Erroneous initiation. The sequence BAD92539.1 differs from that shown. Reason: Erroneous initiation.