VRK3 Recombinant Protein

Name: VRK3 Recombinant Protein; Research Grade; MyBioSource
Brand: MyBioSource
SKU: MBS515272
Price: 350 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS515272

Recombinant full-length human VRK3 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.Scientific Background: VRK3 is a member of the vaccinia-related kinase (VRK) family of serine/threonine protein kinases which has substitutions at… More details

$350 / 0.02 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

VRK3, Recombinant Protein

Full Product Name

VRK3 recombinant protein

Product Gene Name

VRK3 recombinant protein

Host

Sf9 insect cells

Form/Format

50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.

Preparation and Storage

Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Molecular Weight

~80 kDa

Chromosome Location

Chromosome: 19; NC_000019.9 (50479724..50529274, complement). Location: 19q13

3D Structure

ModBase 3D Structure for Q8IV63

Type

Recombinant Fusion Protein

Species

Human

Tag Information

GST tag

Expression System

Sf9 insect cells using baculovirus

Source Note

Recombinant full-length human VRK3 was expressed by baculovirus in Sf9 insect cells

Dry Ice Shipment

Extra charge fee may add to your shipping cost as dry ice is required to ship this product.

Product Categories/Family for VRK3 recombinant protein

Cellular Proteins; Signaling Proteins - Cellular Proteins

Applications Tested/Suitable for VRK3 recombinant protein

Western Blot (WB)

Product References and Citations for VRK3 recombinant protein

1. Scheeff, E D. et al: Structure of the pseudokinase VRK3 reveals a degraded catalytic site, a highly conserved kinase fold, and a putative regulatory binding site. Structure. 2009 Jan 14;17(1):128-38.
2. Sanz-Garci-a M, et.al: Proteomics identification of nuclear Ran GTPase as an inhibitor of human VRK1 and VRK2 (vaccinia-related kinase) activities. Mol Cell Proteomics. 2008 Nov;7(11):2199-214.

Other Notes

Small volumes of VRK3 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for VRK3 purchase

MBS515272 is a recombinant protein targeting VRK3. Host: Sf9 insect cells.

Additional Details

NCBI/Uniprot data below describe general gene information for VRK3. It may not necessarily be applicable to this product.

NCBI GI #

71164885

NCBI GeneID

51231

NCBI Accession #

NP_057524.3

UniProt Primary Accession #

Q8IV63

UniProt Secondary Accession #

Q502Y2; Q9P2V8; A6NEG5; A8KA53

UniProt Related Accession #

Q8IV63

GenBank Nucleotide #

NM_016440

NCBI Official Full Name

Homo sapiens vaccinia related kinase 3 (VRK3), transcript variant 1, mRNA

NCBI Official Synonym Full Names

vaccinia related kinase 3

NCBI Official Symbol

VRK3

UniProt Protein Name

Inactive serine/threonine-protein kinase VRK3

UniProt Synonym Protein Names

Serine/threonine-protein pseudokinase VRK3; Vaccinia-related kinase 3

Protein Family

Inactive serine/threonine-protein kinase

UniProt Gene Name

VRK3

UniProt Entry Name

VRK3_HUMAN

NCBI Protein Information

inactive serine/threonine-protein kinase VRK3; vaccinia-related kinase 3; serine/threonine-protein pseudokinase VRK3

NCBI Summary for VRK3

This gene encodes a member of the vaccinia-related kinase (VRK) family of serine/threonine protein kinases. In both human and mouse, this gene has substitutions at several residues within the ATP binding motifs that in other kinases have been shown to be required for catalysis. In vitro assays indicate the protein lacks phosphorylation activity. The protein, however, likely retains its substrate binding capability. This gene is widely expressed in human tissues and its protein localizes to the nucleus. Alternative splicing results in multiple transcripts encoding different isoforms. [provided by RefSeq, Jul 2008]

UniProt Comments for VRK3

Function: Inactive kinase that suppresses ERK activity by promoting phosphatase activity of DUSP3 which specifically dephosphorylates and inactivates ERK in the nucleus

By similarity. Ref.6 Ref.12

Subunit structure: Interacts with DUSP3

By similarity. Interacts with RAN. Ref.7

Subcellular location: Nucleus Ref.6.

Sequence similarities: Belongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family. VRK subfamily.Contains 1 protein kinase domain.

Caution: Inactive as a kinase due to its inability to bind ATP (Ref.12).

Research Articles on VRK3

1. Data from molecular dynamic simulations suggest that bivalent cations play key structural roles in stabilization of VRK1/VRK2; bivalent cations have no obvious effects on VRK3. These kinases are targets in search for antineoplastic agents. [REVIEW]

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with VRK3 recombinant protein

Reference Product	PubMed Publications
VRK1 recombinant protein	>4 publications with VRK3 and VRK1
DUSP3 recombinant protein	>2 publications with VRK3 and DUSP3

Organs/Tissues associated with VRK3 recombinant protein

Organ/Tissue Name	Pubmed Publications
Nerve Antibodies	>2 publications with VRK3 and Nerve
Embryonic Tissue Antibodies	>2 publications with VRK3 and Embryonic Tissue
Spleen Antibodies	>1 publications with VRK3 and Spleen
Liver Antibodies	>1 publications with VRK3 and Liver
Thymus Antibodies	>1 publications with VRK3 and Thymus
Blood Antibodies	>1 publications with VRK3 and Blood
Testis Antibodies	>1 publications with VRK3 and Testis