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UBE2F Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS515278

Recombinant full length human UBE2F was expressed in E. coli cells using an N-terminal His tag.Scientific Background: UBE2F or ubiquitin-conjugating enzyme E2F is part of the ubiquitin network for protein degradation and is involved in protein neddylation.… More details

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Testing Data View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
UBE2F, Recombinant Protein
Full Product Name
UBE2F recombinant protein
Product Gene Name
UBE2F recombinant protein
Product Synonym Gene Name
NCE2
Product Synonym Names
NCE2
Host
E Coli
Form/Format
50mM sodium phosphate, pH 7.0, 300mM NaCl, 150mM imidazole, 0.1mM PMSF, 0.25mM DTT, 25% glycerol.
Preparation and Storage
Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Molecular Weight
~24 kDa
Chromosome Location
Chromosome: 2; NC_000002.11 (238875587..238951423). Location: 2q37.3
3D Structure
ModBase 3D Structure for Q969M7
Type
Recombinant Fusion Protein
Species
Human
Tag Information
His tag
Expression System
E.coli
Source Note
Recombinant full length human UBE2F was expressed in E. coli cells
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.

Product Categories/Family for UBE2F recombinant protein

Ubiquitin Proteins; Signaling Proteins - Ubiquitin Proteins
Applications Tested/Suitable for UBE2F recombinant protein
Western Blot (WB)

SDS-PAGE of UBE2F recombinant protein

UBE2F Recombinant Protein – Cat# MBS515278

Other Notes

Small volumes of UBE2F recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for UBE2F purchase

MBS515278 is a recombinant protein targeting UBE2F. Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for UBE2F. It may not necessarily be applicable to this product.

NCBI GI #
323668293
NCBI GeneID
140739
NCBI Accession #
NP_542409.1
UniProt Primary Accession #
Q969M7
UniProt Secondary Accession #
A8K1Z8; B4DDT9; B4DFI1; B4DMK3; B4DZU2; B8ZZG2
UniProt Related Accession #
Q969M7
GenBank Nucleotide #
NM_080678
NCBI Official Full Name
Homo sapiens ubiquitin-conjugating enzyme E2F (putative) (UBE2F), transcript variant 1, mRNA
NCBI Official Synonym Full Names
ubiquitin-conjugating enzyme E2F (putative)
NCBI Official Symbol
UBE2F
NCBI Official Synonym Symbols
NCE2
UniProt Protein Name
NEDD8-conjugating enzyme UBE2F
UniProt Synonym Protein Names
NEDD8 carrier protein UBE2F; NEDD8 protein ligase UBE2F; NEDD8-conjugating enzyme 2; Ubiquitin-conjugating enzyme E2 F
Protein Family
NEDD8-conjugating enzyme
UniProt Gene Name
UBE2F
UniProt Synonym Gene Names
NCE2
UniProt Entry Name
UBE2F_HUMAN
NCBI Protein Information
NEDD8-conjugating enzyme UBE2F; NEDD8 protein ligase UBE2F; NEDD8-conjugating enzyme 2; NEDD8 carrier protein UBE2F

UniProt Comments for UBE2F

Function: Accepts the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins. The specific interaction with the E3 ubiquitin ligase RBX2, but not RBX1, suggests that the RBX2-UBE2F complex neddylates specific target proteins, such as CUL5. Ref.6

Catalytic activity: ATP + NEDD8 + protein lysine = AMP + diphosphate + protein N-NEDD8yllysine.

Pathway: Protein modification; protein neddylation.

Subunit structure: Interacts with UBA3 and RBX2. Ref.6

Tissue specificity: Widely expressed (at protein level). Ref.6

Post-translational modification: The acetylation of Met-1 increases affinity for DCUN1D3 by about 2 orders of magnitude and is crucial for NEDD8 transfer to cullins.

Sequence similarities: Belongs to the ubiquitin-conjugating enzyme family. UBE2F subfamily.
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Research Articles on UBE2F

1. Distinct preferences of UBC12 and UBE2F peptides for inhibiting different DCNLs, including the oncogenic DCNL1.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with UBE2F recombinant protein

 Products by Pathway  Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 Antigen Processing: Ubiquitination & Proteasome Degradation Pathway antibodies  Antigen Processing: Ubiquitination & Proteasome Degradation Pathway Diagram
 Class I MHC Mediated Antigen Processing & Presentation Pathway antibodies  Class I MHC Mediated Antigen Processing & Presentation Pathway Diagram
 Immune System Pathway antibodies  Immune System Pathway Diagram
 Integrated Breast Cancer Pathway antibodies  Integrated Breast Cancer Pathway Diagram
 Ubiquitin Mediated Proteolysis Pathway antibodies  Ubiquitin Mediated Proteolysis Pathway Diagram
 Ubiquitin Mediated Proteolysis Pathway antibodies  Ubiquitin Mediated Proteolysis Pathway Diagram

Organs/Tissues associated with UBE2F recombinant protein

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >1 publications with UBE2F and Blood
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