MGRN1 Recombinant Protein

Function: E3 ubiquitin-protein ligase. Mediates monoubiquitination at multiple sites of TSG101 in the presence of UBE2D1, but not of UBE2G1, nor UBE2H. Plays a role in the regulation of endosome-to-lysosome trafficking. Impairs MC1R- and MC4R-signaling by competing with GNAS-binding to MCRs and inhibiting agonist-induced cAMP production. Does not inhibit ADRB2-signaling. Does not promote MC1R ubiquitination. Ref.1 Ref.5 Ref.7

Pathway: Protein modification; protein ubiquitination.

Subunit structure: Interacts with MC1R and MC4R, but not with TBXA2R. Interacts with TSG101. Interacts with mislocalized cytosolically exposed PRNP; this interaction alters MGRN1 subcellular location and causes lysosomal enlargement

By similarity. Ref.1 Ref.5 Ref.7

Subcellular location: Early endosome. Note: The endosomal localization is dependent on the interaction with TSG101. Ref.1 Ref.5Isoform 1: Cytoplasm › cytosol. Nucleus. Note: Translocation from the cytosol to the nucleus is seen only in the presence of MC1R and MC4R, but not TBXA2R. Excluded from nucleoli. Ref.1 Ref.5Isoform 2: Cytoplasm › cytosol. Nucleus. Note: Translocation from the cytosol to the nucleus is seen only in the presence of MC1R and MC4R, but not TBXA2R. Excluded from nucleoli. Ref.1 Ref.5Isoform 3: Cytoplasm › cytosol. Cell membrane Ref.1 Ref.5. Isoform 4: Cytoplasm › cytosol. Cell membrane Ref.1 Ref.5.

Domain: The RING finger is required for ubiquitin ligase activity

By similarity.

Post-translational modification: Autoubiquitinated in vitro

By similarity.

Sequence similarities: Contains 1 RING-type zinc finger.

Sequence caution: The sequence BAA25470.1 differs from that shown. Reason: Erroneous initiation.