SIRT2 Recombinant Protein

This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class I of the sirtuin family. Several transcript variants are resulted from alternative splicing of this gene. [provided by RefSeq, Jul 2010]

Function: NAD-dependent protein deacetylase, which deacetylates internal lysines on histone and non-histone proteins such as ACLY, PCK1 and RELA. Deacetylates 'Lys-40' of alpha-tubulin. Involved in the control of mitotic exit in the cell cycle, probably via its role in the regulation of cytoskeleton. Deacetylates PCK1, opposing proteasomal degradation. Deacetylates 'Lys-310' of RELA. Ref.12 Ref.13 Ref.20 Ref.21 Ref.22

Catalytic activity: NAD+ + an acetylprotein = nicotinamide + O-acetyl-ADP-ribose + a protein. Ref.11

Cofactor: Binds 1 zinc ion per subunit.

Enzyme regulation: Inhibited by Sirtinol, A3 and M15 small molecules. Inhibited by nicotinamide.

Subunit structure: Interacts with HDAC6, suggesting that these proteins belong to a large complex that deacetylate the cytoskeleton. Ref.12

Subcellular location: Cytoplasm › cytoskeleton. Note: Colocalizes with microtubules. Ref.2 Ref.12 Ref.15

Tissue specificity: Widely expressed. Highly expressed in heart, brain and skeletal muscle, while it is weakly expressed in placenta and lung. Down-regulated in many gliomas suggesting that it may act as a tumor suppressor gene in human gliomas possibly through the regulation of microtubule network. Ref.1 Ref.2 Ref.14

Developmental stage: Peaks during mitosis. After mitosis, it is probably degraded by the 26S proteasome. Ref.13

Post-translational modification: Phosphorylated at the G2/M transition of the cell cycle. Ref.13

Miscellaneous: Has some ability to deacetylate histones in vitro, but seeing its subcellular location, this is unlikely in vivo.

Sequence similarities: Belongs to the sirtuin family. Class I subfamily.Contains 1 deacetylase sirtuin-type domain.

Sequence caution: The sequence AAD45971.1 differs from that shown. Reason: Erroneous initiation. The sequence AAF67015.1 differs from that shown. Reason: Frameshift at several positions.