SMYD3 Recombinant Protein

Name: SMYD3 Recombinant Protein; Research Grade; MyBioSource
Brand: MyBioSource
SKU: MBS515342
Price: 345 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS515342

Recombinant full-length human SMYD3 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.Scientific Background: SMYD3 (SET and MYND domain containing 3) is a histone methyltransferase that plays a role in transcriptional regulation as… More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Product Name

SMYD3, Recombinant Protein

Full Product Name

SMYD3 recombinant protein

Product Gene Name

SMYD3 recombinant protein

Product Synonym Gene Name

ZMYND1; ZNFN3A1; FLJ21080

Product Synonym Names

ZMYND1; ZNFN3A1; FLJ21080

Host

Sf9 insect cells

Form/Format

50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.

Preparation and Storage

Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Molecular Weight

~71 kDa

Chromosome Location

Chromosome: 1; NC_000001.10 (245912642..246670644, complement). Location: 1q44

OMIM

608783

3D Structure

ModBase 3D Structure for Q9H7B4

Type

Recombinant Fusion Protein

Species

Human

Tag Information

GST tag

Expression System

Sf9 insect cells using baculovirus

Source Note

Recombinant full-length human SMYD3 was expressed by baculovirus in Sf9 insect cells

Product Categories/Family for SMYD3 recombinant protein

Acetyl/Methyltransferases Proteins; Signaling Proteins - Acetyl/Methyltransferase Proteins

Applications Tested/Suitable for SMYD3 recombinant protein

Western Blot (WB)

Product References and Citations for SMYD3 recombinant protein

1. Hamamoto, R. et.al: SMYD3 encodes a histone methyltransferase involved in the proliferation of cancer cells. Nature Cell Biol. 6: 731-740, 2004.
2. Tsuge, M. et.al: A variable number of tandem repeats polymorphism in an E2F-1 binding element in the 5-prime flanking region of SMYD3 is a risk factor for human cancers. Nature Genet. 37: 1104-1107, 2005.

Other Notes

Small volumes of SMYD3 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for SMYD3 purchase

MBS515342 is a recombinant protein targeting SMYD3. Host: Sf9 insect cells.

Additional Details

NCBI/Uniprot data below describe general gene information for SMYD3. It may not necessarily be applicable to this product.

NCBI GI #

21410973

NCBI GeneID

64754

NCBI Accession #

BC031010

UniProt Primary Accession #

Q9H7B4

UniProt Secondary Accession #

Q86TL8; Q8N5Z6; Q96AI5; A8K0P0; B1AN38

UniProt Related Accession #

Q9H7B4

NCBI Official Full Name

Homo sapiens SET and MYND domain containing 3, mRNA

NCBI Official Synonym Full Names

SET and MYND domain containing 3

NCBI Official Symbol

SMYD3

NCBI Official Synonym Symbols

KMT3E; ZMYND1; ZNFN3A1; bA74P14.1

UniProt Protein Name

Histone-lysine N-methyltransferase SMYD3

UniProt Synonym Protein Names

SET and MYND domain-containing protein 3; Zinc finger MYND domain-containing protein 1

Protein Family

Histone-lysine N-methyltransferase

UniProt Gene Name

SMYD3

UniProt Synonym Gene Names

ZMYND1; ZNFN3A1

UniProt Entry Name

SMYD3_HUMAN

NCBI Protein Information

histone-lysine N-methyltransferase SMYD3; bA74P14.1 (novel protein); zinc finger, MYND domain containing 1; SET and MYND domain-containing protein 3; zinc finger MYND domain-containing protein 1; zinc finger protein, subfamily 3A (MYND domain containing), 1

NCBI Summary for SMYD3

This gene encodes a histone methyltransferase which functions in RNA polymerase II complexes by an interaction with a specific RNA helicase. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011]

UniProt Comments for SMYD3

Function: Histone methyltransferase. Specifically methylates 'Lys-4' and 'Lys-5' of histone H3, inducing di- and tri-methylation, but not monomethylation. Plays an important role in transcriptional activation as a member of an RNA polymerase complex. Binds DNA containing 5'-CCCTCC-3' or 5'-GAGGGG-3' sequences. Ref.1 Ref.8

Catalytic activity: S-adenosyl-L-methionine + L-lysine-[histone] = S-adenosyl-L-homocysteine + N(6)-methyl-L-lysine-[histone]. Ref.1 Ref.8

Enzyme regulation: Histone methyltransferase activity strongly stimulated by HSPCA.

Subunit structure: Interacts with HSPCA. Interacts with HELZ. Interacts with POLR2A; the interaction may be indirect and may be mediated by HELZ. Ref.1

Subcellular location: Cytoplasm. Nucleus. Note: Mainly cytoplasmic when cells are arrested at G0/G1. Accumulates in the nucleus at S phase and G2/M. Ref.1

Tissue specificity: Expressed in skeletal muscles and testis. Overexpressed in a majority of colorectal and hepatocellular carcinomas. Ref.1

Sequence similarities: Belongs to the class V-like SAM-binding methyltransferase superfamily. Histone-lysine methyltransferase family.Contains 1 MYND-type zinc finger.Contains 1 SET domain.

Sequence caution: The sequence CAI14744.1 differs from that shown. Reason: Erroneous initiation. The sequence CAI16628.1 differs from that shown. Reason: Erroneous initiation.

Research Articles on SMYD3

1. low miR-124 levels mediated by HCV core protein via DNMT1 promote intrahepatic cholangiocarcinoma cell migration and invasion by targeting SMYD3

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with SMYD3 recombinant protein

Reference Product	PubMed Publications
MET recombinant protein	>2 publications with SMYD3 and MET
HELZ recombinant protein	>2 publications with SMYD3 and HELZ
RASSF1 recombinant protein	>1 publications with SMYD3 and RASSF1

Diseases associated with SMYD3 recombinant protein

Disease Name	Pubmed Publications
Neoplasms Antibodies	>33 publications with SMYD3 and Neoplasms
Adenocarcinoma Antibodies	>12 publications with SMYD3 and Adenocarcinoma
Carcinoma, Hepatocellular Antibodies	>12 publications with SMYD3 and Carcinoma, Hepatocellular
Liver Neoplasms Antibodies	>10 publications with SMYD3 and Liver Neoplasms
Breast Neoplasms Antibodies	>8 publications with SMYD3 and Breast Neoplasms
Inflammation Antibodies	>2 publications with SMYD3 and Inflammation
Cell Transformation, Neoplastic Antibodies	>2 publications with SMYD3 and Cell Transformation, Neoplastic
Liver Neoplasms, Experimental Antibodies	>1 publications with SMYD3 and Liver Neoplasms, Experimental
Disease Models, Animal Antibodies	>1 publications with SMYD3 and Disease Models, Animal
Carcinoma, Squamous Cell Antibodies	>1 publications with SMYD3 and Carcinoma, Squamous Cell

Organs/Tissues associated with SMYD3 recombinant protein

Organ/Tissue Name	Pubmed Publications
Liver Antibodies	>14 publications with SMYD3 and Liver
Muscle Antibodies	>7 publications with SMYD3 and Muscle
Heart Antibodies	>6 publications with SMYD3 and Heart
Embryonic Tissue Antibodies	>2 publications with SMYD3 and Embryonic Tissue
Lung Antibodies	>2 publications with SMYD3 and Lung
Prostate Antibodies	>1 publications with SMYD3 and Prostate
Bone Marrow Antibodies	>1 publications with SMYD3 and Bone Marrow
Vascular Antibodies	>1 publications with SMYD3 and Vascular
Blood Antibodies	>1 publications with SMYD3 and Blood
Bone Antibodies	>1 publications with SMYD3 and Bone