PFKFB1 Recombinant Protein

Name: PFKFB1 Recombinant Protein; Research Grade; MyBioSource
Brand: MyBioSource
SKU: MBS515359
Price: 345 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS515359

Recombinant full-length human PFKFB1 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.Scientific Background: PFKFB1 is a member of the family of bifunctional 6-phosphofructo-2-kinase: fructose-2, 6-biphosphatase enzymes which forms… More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Product Name

PFKFB1, Recombinant Protein

Full Product Name

PFKFB1 recombinant protein

Product Gene Name

PFKFB1 recombinant protein

Product Synonym Gene Name

F6PK; HL2K; PFRX

Product Synonym Names

F6PK; HL2K; PFRX; PFKFB1 protein

Host

Sf9 insect cells

Form/Format

50mM Tris-HCl, pH 7.5, 50mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.

Preparation and Storage

Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Molecular Weight

~80 kDa

Chromosome Location

Chromosome: X; NC_000023.10 (54959307..55025244, complement). Location: Xp11.21

OMIM

311790

3D Structure

ModBase 3D Structure for P16118

Type

Recombinant Fusion Protein

Species

Human

Tag Information

GST tag

Expression System

Sf9 insect cells using baculovirus

Source Note

Recombinant full-length human PFKFB1 was expressed by baculovirus in Sf9 insect cells

Product Categories/Family for PFKFB1 recombinant protein

Fructose Kinases Proteins; Signaling Proteins - Fructose Kinase Proteins

Applications Tested/Suitable for PFKFB1 recombinant protein

Western Blot (WB)

Product References and Citations for PFKFB1 recombinant protein

1. Algaier, J.et.al: Molecular cloning, sequence analysis, and expression of a human liver cDNA coding for fructose-6,2-kinase:fructose-2,6-bisphosphatase. Biochem. Biophys. Res. Commun. 153: 328-333, 1988.
2. Darville, M. I. et.al: Complete nucleotide sequence coding for rat liver 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase derived from a cDNA clone. FEBS Lett. 224: 317-321, 1987.

Other Notes

Small volumes of PFKFB1 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PFKFB1 purchase

MBS515359 is a recombinant protein targeting PFKFB1. Host: Sf9 insect cells.

Additional Details

NCBI/Uniprot data below describe general gene information for PFKFB1. It may not necessarily be applicable to this product.

NCBI GI #

424036665

NCBI GeneID

5207

NCBI Accession #

NP_002616.2

UniProt Primary Accession #

P16118

UniProt Secondary Accession #

Q5JXS5; Q99951; B2RA88

UniProt Related Accession #

P16118

GenBank Nucleotide #

NM_002625

NCBI Official Full Name

Homo sapiens 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1 (PFKFB1), transcript variant 1, mRNA

NCBI Official Synonym Full Names

6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1

NCBI Official Symbol

PFKFB1

NCBI Official Synonym Symbols

F6PK; HL2K; PFRX

UniProt Protein Name

6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 1

UniProt Synonym Protein Names

6PF-2-K/Fru-2,6-P2ase liver isozyme

Protein Family

6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase

UniProt Gene Name

PFKFB1

UniProt Synonym Gene Names

F6PK; PFRX; 6PF-2-K/Fru-2,6-P2ase 1

UniProt Entry Name

F261_HUMAN

NCBI Protein Information

6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 1; PFK/FBPase 1; 6PF-2-K/Fru-2,6-P2ase 1; 6PF-2-K/Fru-2,6-P2ASE liver isozyme; fructose-6-phosphate,2-kinase:fructose-2,6-bisphosphatase

NCBI Summary for PFKFB1

This gene encodes a member of the family of bifunctional 6-phosphofructo-2-kinase:fructose-2,6-biphosphatase enzymes. The enzyme forms a homodimer that catalyzes both the synthesis and degradation of fructose-2,6-biphosphate using independent catalytic domains. Fructose-2,6-biphosphate is an activator of the glycolysis pathway and an inhibitor of the gluconeogenesis pathway. Consequently, regulating fructose-2,6-biphosphate levels through the activity of this enzyme is thought to regulate glucose homeostasis. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Nov 2012]

UniProt Comments for PFKFB1

Function: Synthesis and degradation of fructose 2,6-bisphosphate.

Catalytic activity: Beta-D-fructose 2,6-bisphosphate + H2O = D-fructose 6-phosphate + phosphate.ATP + D-fructose 6-phosphate = ADP + beta-D-fructose 2,6-bisphosphate.

Enzyme regulation: Phosphorylation results in inhibition of the kinase activity.

Subunit structure: Homodimer. Ref.6

Tissue specificity: Liver.

Sequence similarities: In the C-terminal section; belongs to the phosphoglycerate mutase family.

Research Articles on PFKFB1

1. Observational study of genetic testing. (HuGE Navigator)

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with PFKFB1 recombinant protein

Reference Product	PubMed Publications
PFKM recombinant protein	>1 publications with PFKFB1 and PFKM

Pathways associated with PFKFB1 recombinant protein

Products by Pathway	Pathway Diagram
Fructose And Mannose Metabolism Pathway antibodies	Fructose And Mannose Metabolism Pathway Diagram
Fructose And Mannose Metabolism Pathway antibodies	Fructose And Mannose Metabolism Pathway Diagram
Gluconeogenesis Pathway antibodies	Gluconeogenesis Pathway Diagram
Glucose Metabolism Pathway antibodies	Glucose Metabolism Pathway Diagram
Glycolysis Pathway antibodies	Glycolysis Pathway Diagram
HIF-1 Signaling Pathway antibodies	HIF-1 Signaling Pathway Diagram
Integration Of Energy Metabolism Pathway antibodies	Integration Of Energy Metabolism Pathway Diagram
Metabolism Pathway antibodies	Metabolism Pathway Diagram
Metabolism Of Carbohydrates Pathway antibodies	Metabolism Of Carbohydrates Pathway Diagram
PKA-mediated Phosphorylation Of Key Metabolic Factors Pathway antibodies	PKA-mediated Phosphorylation Of Key Metabolic Factors Pathway Diagram

Diseases associated with PFKFB1 recombinant protein

Disease Name	Pubmed Publications
Cell Transformation, Neoplastic Antibodies	>2 publications with PFKFB1 and Cell Transformation, Neoplastic
Nervous System Diseases Antibodies	>1 publications with PFKFB1 and Nervous System Diseases
Carcinoma Antibodies	>1 publications with PFKFB1 and Carcinoma
Adenocarcinoma Antibodies	>1 publications with PFKFB1 and Adenocarcinoma
Weight Gain Antibodies	>1 publications with PFKFB1 and Weight Gain
Neoplasms, Experimental Antibodies	>1 publications with PFKFB1 and Neoplasms, Experimental
Brain Diseases Antibodies	>1 publications with PFKFB1 and Brain Diseases

Organs/Tissues associated with PFKFB1 recombinant protein

Organ/Tissue Name	Pubmed Publications
Liver Antibodies	>8 publications with PFKFB1 and Liver
Muscle Antibodies	>4 publications with PFKFB1 and Muscle
Heart Antibodies	>2 publications with PFKFB1 and Heart