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UBLE1A (SAE1) Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS515376

Recombinant full-length human UBLE1A (SAE1) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.Scientific Background: UBLE1A (also known as SAE1) or SUMO1 activating enzyme subunit 1 is involved in regulating protein structure and… More details

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Product Overview

Online Datasheet in PDF
Product Name
UBLE1A (SAE1), Recombinant Protein
Full Product Name
UBLE1A (SAE1) recombinant protein
Product Synonym Gene Name
SAE1; SUA1; HSPC140; AOS1; FLJ3091; HSPC140
Product Synonym Names
SAE1; SUA1; HSPC140; AOS1; FLJ3091; HSPC140; UBLE1A(SAE1) protein
Host
Sf9 insect cells
Form/Format
50mM Tris-HCl, pH 7.5, 50mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
Preparation and Storage
Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Molecular Weight
~65 kDa
Chromosome Location
Chromosome: 19; NC_000019.9 (47634080..47713893). Location: 19q13.32
OMIM
613294
3D Structure
ModBase 3D Structure for Q9UBE0
Type
Recombinant Fusion Protein
Species
Human
Tag Information
GST tag
Expression System
Sf9 insect cells using baculovirus
Source Note
Recombinant full-length human UBLE1A (SAE1) was expressed by baculovirus in Sf9 insect cells
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Applications Tested/Suitable for UBLE1A (SAE1) recombinant protein
Western Blot (WB)

SDS-PAGE of UBLE1A (SAE1) recombinant protein

The purity of UBLE1A (SAE1) was determined to be >95% by densitometry. Approx. MW 65kDa
UBLE1A (SAE1) Recombinant Protein – Cat# MBS515376

Product References and Citations for UBLE1A (SAE1) recombinant protein

1. Okuma, T. et.al: In vitro SUMO-1 modification requires two enzymatic steps, E1 and E2. Biochem. Biophys. Res. Commun. 254: 693-698, 1999.
2. Kessler, J. D. et.al: A SUMOylation-dependent transcriptional subprogram is required for Myc-driven tumorigenesis. Science 335: 348-353, 2012.

Other Notes

Small volumes of UBLE1A (SAE1) recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for UBLE1A (SAE1) purchase

MBS515376 is a recombinant protein targeting UBLE1A (SAE1). Host: Sf9 insect cells.

Additional Details

NCBI/Uniprot data below describe general gene information for UBLE1A (SAE1). It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
Homo sapiens SUMO1 activating enzyme subunit 1 (SAE1), transcript variant 1, mRNA
NCBI Official Synonym Full Names
SUMO1 activating enzyme subunit 1
NCBI Official Symbol
SAE1
NCBI Official Synonym Symbols
AOS1; SUA1; UBLE1A; HSPC140
UniProt Protein Name
SUMO-activating enzyme subunit 1
UniProt Synonym Protein Names
Ubiquitin-like 1-activating enzyme E1A
UniProt Gene Name
SAE1
UniProt Synonym Gene Names
AOS1; SUA1; UBLE1A
UniProt Entry Name
SAE1_HUMAN
NCBI Protein Information
SUMO-activating enzyme subunit 1; activator of SUMO1; SUMO-1 activating enzyme subunit 1; sentrin/SUMO-activating protein AOS1; SUMO-1 activating enzyme E1 N subunit; ubiquitin-like 1-activating enzyme E1A; ubiquitin-like protein SUMO-1 activating enzyme

NCBI Summary for UBLE1A (SAE1)

Posttranslational modification of proteins by the addition of the small protein SUMO (see SUMO1; MIM 601912), or sumoylation, regulates protein structure and intracellular localization. SAE1 and UBA2 (MIM 613295) form a heterodimer that functions as a SUMO-activating enzyme for the sumoylation of proteins (Okuma et al., 1999 [PubMed 9920803]).[supplied by OMIM, Mar 2010]
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UniProt Comments for UBLE1A (SAE1)

Function: The heterodimer acts as a E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2. Ref.1 Ref.2 Ref.3 Ref.11 Ref.12 Ref.16 Ref.17

Pathway: Protein modification; protein sumoylation.

Subunit structure: Heterodimer of SAE1 and UBA2/SAE2. The heterodimer corresponds to the two domains that are encoded on a single polypeptide chain in ubiquitin-activating enzyme E1. Interacts with UBE2I. Ref.1 Ref.2 Ref.3 Ref.11

Subcellular location: Nucleus Ref.11.

Tissue specificity: Expression level increases during S phase and drops in G2 phase (at protein level). Ref.11

Sequence similarities: Belongs to the ubiquitin-activating E1 family.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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